this experiment, you will vary the the substrate — pera“ and eee Sethe cndag, nee meee ‘The catalase inthis tissue. Instead of of measuring the ‘you will count th ‘given off from 1 = experiment comes from the potato ‘sing floating filter paper asa way ‘ale at which oxygen is given off, 1¢ rate at which bubbles of oxygen are the mixture in the test tube. Set up the apparatus shown in the diagram. ‘lass tube ee water tube2 | a Ea 5 Cutten thn (1-2 mm thick) compet (diss) froma potato cylinder. Place in tube 1. Replace the bung tightly. shake the tube to separate the slices, 6 Wai seconds and then count the bubbles produced in 1 minute. 7 Prepare a results table as shown below, the st number Concent ‘nme dnt : os To [as Tein aie [tenure eatin ntbiles [indeount ermine weet ein 8 Discard the contents of tube 1, wash tepeat steps 5 and 6 twice. Record in numbers of bubbles produced. 9 Repeat the experiment using 1.0, 1.5 20mol/dm’H,0,, Record the table and calculate means for each c 10 Draw a graph to show the mean na er es 1Suggest ahypothess that was being tested invesigvon 2. From you results what were the effect ofthe conceniration ofthe substrate onthe at of reaction? 3 How do you erin you rests? 4. Why was tube shaken to separate theses? 5 Why mer the sizes changed after taking ot «count. istead of taking tre counts with etch lees? 6 Suggest he main tations ofthe method ‘experiment. and how these may have affected rely of your rests