Live Cell Imaging Microscope Evaluation LEICA Name:

Please answer the questions below. Please provide comments about features you like or dont like, features that are
needed but absent; features that dont work or are not needed.

1. Quality of image at low mag through plastic cultureware (2x-20x)

2. Quality of image at high mag through glass coverslip (40x-100x)

3. Size of image field (does the system capture images at a size appropriate for your work at the magnification needed)

4. Image montaging/stitching (automated capture and assembly of adjacent fields to form a larger image of the whole)

5. Repeatability of sampling in X-Y plane

6. Repeatability of focus in time lapse, focus stability and autofocus.

7. Extended focus function.

8. Stage mounted incubation system.

9. Contrast Imaging (phase contrast, modulation contrast)

10. Software (Ease of use; Stability; Are the functions needed present in the software package/what additional functions
are needed?).

11. Stage-mounted culture incubation system (Tokai Hit system)