OLYMPUS INSTRUCTIONS CX31-P POLARIZING MICROSCOPE Tis instruction manual is for the Olympus Polarizing Microscopes Model CX31-P To ensure the Steg, obtain opmum perfomance and te famarze yoursel uy stn ine use of hs mioscons, vee recommend thatyou study tie manual thoroughly before operating tne microscope. Rolain His Instruction manual in an easily accessible place near the work desk for MureCX31-P CONTE. Conect assembly and adjustments are citical for the microscope to ranifest its full performance, If you are going to assemble the microscope by yourself, please read Chapter 9, “ASSEMBLY” (Pages 23 to 26) For the assemblies of the ‘modules for which instruction manuals are available, refer to their instruction manuals, IMPORTANT - Be sure to read this first for safe use of the equipment. — 13 1 MODULE NOMENCLATURE 4 2 CONTROLS 5 3 SUMMARY OF POLARIZED LIGHT OBSERVATION PROCEDURE 6-7 4 OPERATION B44 4-1 Microscope Frame i Teming the Lamp On © Fae tis Diaphragm © ising the Fiters 4-2. Focusing MOdUIC neem - 9 © Adusting the Coarse Adjustment Knob Rotation Tension © Using the Simplitied Pre-tocusing Knob 4-3. Observation Tube. . enn 9-10 © Adjusting the interbupitary Distance MJ Adjusting the Diopter © Using the Eye Shades Mounting the Eyepiece Micrometer Disk 4-4 Intermediate Tube for Polarizing Observation (U-PA) . " GB Using the Benrands Lens © Using the Anatyzer 4-5 Rotary Stage eo a o an 12 Gi Piacing Specimen on the Stage © Fotating the stage * 4-6 Condenser. ann 13 GH Contering the Field is Diaphragm MJ Using the Aperture rs Diaphragm 47 Immersion Objective...Th ee 5 POLARIZED LIGHT OBSERVATION 15418 5-1 Preparation ... a nents AT GH Cross-Nicol Adjustment © Contering the Rotary Stage © Acivsting the Centering Adapter {or Objective 5-2 Orthoscopic Observation —. 18 5-3 Conoscopic Observation. 18 6 SPECIFICATIONS 19 7 OPTICAL CHARACTERISTICS 20 8 TROUBLESHOOTING GUIDE 21-22 9 ASSEMBLY i 23-26 II PROPER SELECTION OF THE POWER SUPPLY CORD --—---—-2on---2-o~ 27-28CX31-P IMPORTANT 1. After the equipment has been used in an observation ofa specimen that is accompanied with a potential of infection, clean the parls coming in ‘contact with the specimen to prevent infection. * When moving the microscope, be sure to rernove the specimen to prevent it rom dropping and scattering, and hold the microscope by the positions shown in Fig. 2 on the next page. * In case the specimen is damaged by mistake, promptly take the action {or preventing infection, * The microscope becomes unstable when is height is increased by allached modules. in this case, be sure fo take countermeasures for preventing it om toppling down and dropoing the specimen. 2. Toad potential shockhazarts and bums when replacing the lamp bulb, set the main swich © to" (OFF then disconnect the power cord fr tho wall couletin advance and, whenever you replace the bub duing use or ight ater se, alow the lamp replacement cover @ and! bulb to cool below touching. 8 Install te microscope on a sturdy, evel table or bench so as not to block the air vents on the underside ofthe base. Do not place the microscope (on a soft surface into which the microscope may sink as this could result in blocking the air vents and cause overheating ora fie. 4. Always use the power cord provided by Olympus. no power cord is provided, please select the proper power cord by releting to the section "PROPER SELECTION OF THE POWER SUPPLY CORD" at the end of this instruction manual If the proper power cord is not used, product safety performance cannot be warranted. 5. When instaling the microscope, route the power cord away from the ‘microscope ftame. Should the power cord come in contact with @ hot part the power cord could mett and cause electric shack 66 Always ensure that the grounding terrinal ofthe microscope and that ofthe ‘wal out are propery connected ithe equipments not grounded, Olympus ‘can no ionger waren the electrical safety perlornance of the equipment 7 Never set the main switch © to" I" (ON) while a metalic object is present in the airvents of the miceoscope, as this could result in electrical shock, personal injury and equipment damage. {ter operation orn case of abnormally be sure to disconnect the power ‘cord from the connector on the microscope or ftom the wall power outlet Safety Symbols < The folowing symbols are found on the microscope. Study the meaning of the symbols and always use the equipment in the safest possible manner a lndicates tha the suface becomes hot, and should not be touched with bare hands, Before use, carefully ead the instruction manual Improper use could resultin personal injury to A the user andor damage tothe equipment. I Indicates that the main switch is ON, ° Incas thatthe main st is OFFWarning Label ‘A waming indication label Is attached fo every part where special precaution is required when handling and using the microscope. Aivays heed the wamings. ain ; Warning againsthigh tempera Warring label postion | Bottom of microscope tame [Warning agsinen gn sre: | AN, Is the waming label is stained or peeled off, contact Olympus. GE Getting Ready 1. microscope Is a precision instrument, Handle it with care and avoid subjecting it to sudden or severe impact. [Link] not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations. (For the operating cond tions, see chapter 6, “SPECIRCATIONS" on page 19) ‘3, Always use the tension adjustment rng to-adjust the rotation tension of the coarse adustment knob, [Link] microscope is ventilated by natural convection. Be sure 10 leave ‘enough spaces (10 cm or more) around it when instaling it '5. When canying the microsoope, hold itby the bottom of the base @ and finger hook on the tear @ as shown on the lft and cary carefully. To prevent damage, do not hold the microscope by the stage or observation tube, Before carrying, remove the specimen and fiters to prevent them from dropping. ‘x Ifthe microscope is displaced by sliding on the desktop, the rubber feet may be damaged or separated from the bottom. Fig? Maintenance and Storage «To clean the lenses ander ass Component spy blow dy away ung a commercial avaliable blower and wine gently using a piece of cleaning paper (or clean gauze. alone sine wth ngerprns or ol smudges, alco ‘aSince the absolute alcohol is highly fammable, i must be handled caret. See recs amay tom epen fames or potential sources of elecical sparks — for example, electical equipment thats being switched on or of Ako omomber to always use it onlin a wel-ventated room. oan erento us ais cavers 0 clean he microscope components che than the glass components To clean trem uge.a nt#ee sot ah sighly mostened wih a dled noutaldeegent a oot scssobe any part of he miroscope as ths cous est in matuncion or eeuced perormance a fine toting ne meroscope enero thal te frame s cooled down and str it in a ty locker or covert wih a dust cove. aaa veg of he mlroscope check the equations andres of you lal goverment and be sue © obsone them it gauze slightly moistened with commercially available absoluteCX31-P Caution Ifthe microscope is used in a manner not specified by this manual, the safety ofthe user may be [Link] addition, the equipment may also be damaged, Always use the equipment as outlined inthis instruction manual The folowing symbols are used to set of tex in ths instruction manual A: Indicates that falure to follow the instructions in the waming could resutt in bodily harm to the User and/or damage to equipment (including objecs inthe vicinity ofthe equipment. 4 : Indicates that falure to follaw the instructions could resutt in damage to equipment. ©: Indicates commentary for ease of operation and maintenancel Intended use This instrument has been designed to be used to observe magnified rages of specimens in various routine work and research applications, Do not use thi instrument for ary purpose other than its intended use, This instrument is considered a medical device in the following county: US. CE Tis take comptes wih ne stern of iectie S7TEC conceing in vio agro ‘medical devices. CE marking means the conformity tothe directive is equipment has been tested and found to comply with the limits for 2 Ciass A digital device, pursuant fo Part 15 of the FCC Rules. These limits are designed to provide reasonable protection ‘against haimful interference when the equipment is operated in a commercial environment, This equipment generates, uses, and can radiate radio frequency energy and, not installed and used in accordance with the instruction manual, may cause haimful interference to radio communications Operation ofthis equipment in a residental area is key to cause hanful interference in which the user willbe fequited to comect the interference at his own expense. FCC WARNING: Changes or modifications not expressly approved by the patty responsible for compliance could void the users authority to operate the equipment‘©The modules shown below are merely the typical examples For other applicable modules that are not shown, please consult the latest catalogues or Olympus. Other applicable modules ‘+ Mechanical Stage U--MP Tar «+ Rotary Analyzer for Polarized Light U-ANSOOP-2 ‘ AghNTOX/20%/40X/100X0-P + Senstve Tint Plate U-TPS30 + Quarter Wave Plate U-TP137 «Test Piate Adapter U-TAD + Tansmitted Light Analyzer U-ANT + Gout Analyzer U-GAN ‘Compensators (6 models)CX31-P it you have not yet assembled the microscope, read Chapter 9, “ASSEMBLY” (pages 23 to 26) frst. ‘The ilustration shows the microscope together with the U-BIGOP binocular observation tube for polarizing observation UPA intermediate tube for polarizing observation and U-CTAD centering adapter for objective lens. Interpupilay distance scale (Page 9) Diopteradustment ring Analyzer rotation assist knob Page 3) Provided on 2 positions. O “Test plate/compensator insertion slot 7 ‘Anaiyzer clamping knob Page 11) Bertrand’ ens engaging! “Anaiyzor engaging/ttaion knob Page tt) focusing knob Page Tt) eee a0:0ur. ‘Smpifled pre-focusng knob (Page 9) Objective centering scrows (Page 17) Mehewich eee @ ssi ‘Stage rotation clamping knob : - aia ae ‘Aperture iis diaphragm lever (Page 13) Polarizer rotation knob (Page 15), ‘ Fine adjustment knob (Page 9) Felis siaphragm ring (Page 8), Costse adjustment nab (Page 9) \Condenserhelght adlustmentknab Page 13) Filter holder 7a Fe rn there Oscillation direction indications ‘R: Anat Polar‘©This chapter describes the procedure for polarized light observation using the U-PA intermediate tube for polarizing ‘observation. For the procedure using the U-OPA or U:-KPA, refer tothe instruction manual for the intermediate tube in use. '=The operations marked * are not required when the U-CTAD centering adapter for objective lens is not used, ‘Sot the main switch to “I" (ON). Disengage the Bertrand's lens. | - Disengage the analyzor. Place the specimen on the stage. Engage the reference objective inthe ight path. | = Bring the specimen into focus, ‘Adjust the brightness. Adjust the diopter. Adjust the interpupillary distance. Adjust the analyzer and polarizer oscilaton directions. Center the reference objective. Center the stage. Center other objectives. Center the field iris diaphragm. Engage the objective to be used in the light path, engage the analyzer and/or Bertrand's lens according to the purpose of observation, ‘and bring the specimen into focus. (Contro's Used) Page) © Main switch ea ® Light intensity adjustment knob 28) @ Bertrand’ lens engaging/focusing knob (P11) @ Analyzer engaging/rotation knob (P11) .- © Specimen holder “+ © Revowing nosepiece ++ ® Coarsetine foous adjustment knobs 9) ++ ® Light intensity adjustment knob (8) © Binocular tube eo ™ ® Diopter adjustment ing 9 _ @ Analyzer engaging/otation knob 11) ® Polarizer rotation knob en + ® Objective centering screws en @ Stage contering knob P16) ® Objective centering screws em (® Field iis diaphragm ting i) ® Condenser height adjustment knob (P13) ‘© Condenser centering knob PD © Revohing nosepiece __ Analyzer engaging/totaion knob P11) @ Bertrand lens engaging/ocusing knob (P. 11) © Coarserfine focus adjustment knobs (9) senna, B Apeftue is aphagm leer 13) Aetest tive aperture one Rak he Soptrogine, & Feld is diaphragm ring ea Engage the required fters. + ® Fier holder ee ‘Adjust the brightness. + ® Ughtintensiy adjustment knob 8)Observation method | Objective | Bertrand's lens Orthoscopy 410 100K ‘our ‘Conoscopy 20% 10 100%. IN CX31-P ln general biological microscopy, the analyzer, Bertrand fons and test plate are not necessary and should be disengaged ftom the ight path When higher brightness is required. remove the polar izer rotation knob seat ® by puling it downward and take out the polarizer from jnside it @Make a photocopy of the observation procedure pages and post it near your microscope.25 OPERATION Gi Tuning the Lamp on 3.3) 1. Set the main switch © to“ "(OND 2. Rotating the light intensity adjustment knob @ in the direction of the arrow increases brightness and rotating it in the opposite direction decreases brightness, The figures around the knob indicate the refer- cence voltage values. GB Field tis Diaphragm Fo) Rotate the fleld is diaphragm ring @ according to the objective power so that he image ofthe diaphragm circumscrbes the fleld of view. This resticts the diameter of the beam of light entering the objective and thus ‘excludes extraneous light, improving image contrast +s When the 100X objective is used, he iis diaphragm is not visible in the field, In this case, minimize the diaphragm diameter. Using the Filters (Fig. 5) “+ Drop one or stacked #45 mm fits) @ into the window lens on the fame. ‘@ Fo the fiter models, consult the latest catalogues or Olyrnpus.CX31-P. ‘Adjusting the Coarse Adjustment Knob Rotation Tension (Fi. 6) |The tension ofthe couse focus adjustment krob is adustabe. Inset the tip ofa large fat lade screwdver into the groove @ on the teson adjustment ring @ and rotate the ring. Rotating it clockwise (in the direction of he arow)inreses the tension and countactodavse decreases the tension 2. fre stage descends on its own ori the specimen gets out of focus quick even when itis brought into focus using the fre adjustment \eob @, t means thatthe tension ofthe coarse adjustment knob is too low. Turn the ring @ in the direction of the arrow to increase the tension, Using the Simplified Pre-focusing Knob ig.7) ‘* This mechanism does not work when the U-CTAD centering adapter {or objective lens is used. ‘©The pre-ocusing knob controls the mechanism for preventing collision between the specimen and objective. 1. Ate binging the specimen into focus, tum the pre-ocusing knob @ of the {focusing module so that the prefocusing mechanism hits the stage holder 2.7o provide a certain margin for focusing, tum the knob by about a hal {um backward trom the stopped postion. + Ifthe function of this mechanism is not requited, set the pre-focusing knob © at the UP-end position, Adjusting the Interpupillary Distance 8) \While looking through the eyepieces, move both eyepieces until the left and right felds of view coincide completely. The postion of index dot « indicates the interpupilayy distance value. @Note your interpupillay distance so that it can be quickly duplicated. ‘Adjusting the Diopter i 0.9 4. While looking through the right eyepiece with your right eye, tum the ‘coarse and fine focus adjustment knobs to bring the specimen into focus. 2. While looking through the let eyepiece with your ef eye, olate only the iopter adjustment ring @ to focus on the specimen.Using the Eye Shades (Fig. 10) [Wen Wearing Byeaiasses | Use with the eye shades in the normal, folded-down postion. This will prevent the eyeglasses from being scratched. [When Not Wearing Eyeglasses Extend the folded eye shades in the direction ofthe arrow to prevent ‘extraneous light from entering between the eyepieces and eyes. Fig 10 Mounting the Eyepiece Micrometer Disk (Ros. 11 & 12) When the WHN10%H eyepieces are used, en eyepiece micrometer disk can be mounted. Use 24 mm dia. x 15 mm thick micrometer diss. Following Fg. 1, tun the buitin micrometer mounting frame @ to the arrow direction to remove It om the eyeoiece and place a micrometer disk @ into the mounting fame so that the surface with the model ind cation faces downward WHNIOXH The micrometer mounting frame may be tightfor certain micrometer disks, In this case, tum the fame by holding the circumference with a ight, Lniforn force or by applying the flame against a rubber sheet, Do not rasp the frame with a siong force, as this may deform the fame and make itharder to remove it Reatach the micrometer mounting fame in the original position. (@Be careful not to touch the lens surface with your fnget @Wnhen the WHBTOXH for WHBIOX! eyepiaces are usec, an eyepi rricrometer disk with a diameter of $204 mm and thickness of 1 mm Caan be inserted in one of them using the 204RH reticle holders @ (2 piece set. When the reticle holders are used the feld number becomes 196 1, Pace an eyepiece micrometer cisk ® in one of the reticle holders @ so that the engraving on the eyepiece micrometer disk faves downward [Link] the reticle holder @ containing the eyepiece micrometer disk ® into the bottom of the eyepiece, A the end of screwing, turn the reticle holder by hooking your nal on its notch @ to screw it all the way in 8.o provide the other eyepiece withthe sare fled nurnber, sore in the other reticle holder without eyepiece micrometer cs into the bottom of the other eyepiece Fo. WHBIOXHCX31-P. (For the description on the U-OPA or U-KPA intermediate tube for polarizing obsewvation, refer to the Instruction manuel provided with it GB Using the Bertrand’s Lens o.13) 1. To engage the Bertrand’ lens into the light path, slide the Beetrandis lens engaging/focusing knob © horizontally so that the @ [N)indication comes on the front. ‘The Bertand’s lens can be disengaged from the light path by bringing the © (OUT indication on the font. 2.70 adjust the focus of the conoscopic image, turn the Bertrand’s lens engaging/focusing knob ©, GB Using the Analyzer (Fig. 19) 1. To engage the analyzer inthe light path, push in the analyzer engaging/ ‘ialion knob ® (@ positon) ‘The analyzer can be disengaged from the light path by pulling out the knob (0 postion. 2 Loosening the analyzer clamping knob ® makes it possible to rotate the analyzer by up to 180°, . ‘The analyzer can be rotated using the analyzer engaging/totation knob ® or analyzer rotation assist knob ‘The rotation angle can be reac on the rotation scale @. Readout down to @'is possible by using the verier ®, : 1112 @ Piacing Specimen on the Stage When the specimen holders used Fo. 1) lace the specimen on the center and hold with the specimen holder [When the mechanical stage (FWP) Is used Fe [How to attach the mechanical stage Fitthe guide pins on the batiom of the U-FMP into the holes on the stage top, and tighten the clamping screw @ of the U-FMP using an Allen wrench, + While expanding the clamping lever @ of the specimen holder, set the specimen in place by sliding it on the stage. ‘Use a slide glass for mineral specimen (28 x 48 mm) or that for biological specimen (26 x 76 mm). The cover glass thickness should be 0.17 mm. S Mineral specimen Blological specimen. S Rotating the Stage Fo. 16) Loosen the stage rotation clamping knob © to allow the sage to be rotated horizontally by up to 360°. The rion angle can be read on the scale onthe cleumiorence @ (360° partial scale, minimum graduation 1°). Readout down to 6' is pos- + sbie by using the vemiar ©.Fad of view of eyoploce Fig. 18 Fig. 19 CX31-P Gl Centering the Field Iris Diaphragm igs 17 & 16) © When using the U-CTAD centering adapter for objective ens, perform the ‘operation in. @j Adjusting the Centering Adapter for Objective Lens in section 5:1 (page 17) belore proceeding to the folowing steps. 1. Engage the 10x objectve inthe ight path, bring the specimen into focus ‘and rotate the fied iis diaphragm ting @ counterclockwise to stop down the field iris diaphragm sighty. 2.7Tumm the condenser height adjustment knob @ so thatthe image of the field ris diaphragm is focused on the specimen surface ‘[Link] the two condenser centering knobs @ altemately to make the im- age of the diaphragm concentc withthe field 4. Open the fleld iis diaphragm so that is image inscrbes the fel of view. Ifthe image is eccentic, adjust the centering knobs agai 5 Enlarge the iis diaphragm image until it just cicumscribes the lid of view. Using the Aperture Iris Diaphragm (Fig. 19) ©The aperture irs diaphragm adjusts the numerical aperture ofthe ilu ration system. Aligning the numerical aperture © ofthe ilumination sys tem with that ofthe objective in use improves the contrast and resolution 1s well asthe focal depth of he observed image. "How to adjisstthe aperture iris dlaphragm ‘Set the aperture iis dlaphragm lever @ of the condenser so that its numerical aperture @ matches the NA data indicated on the objective. ‘As the aperture irs diaphragm lever has a certain wicth set so that the numerical aperture matches atthe center ofthis width When the 100X oilmmersion objective is used, set the aperture iris diaphragm lever @ to a numerical aperture of 09, ‘As microscopic specimens in genera have low contast it may be opt- ‘mum io sel the aperture iis caphragm to between 70% and BO% ofthe apeture number ofthe objective. Stopping down the aperture iis Giaphragm excessively may result in production of ghost 13i ee 47 Immersion Obectve 14 Gi sing the immersion Objective 0.20) +& Always use immersion oil supplied by Olympus. Using oll other than designated may degrade the condenser lens surface. 4, Focus on the specimen using ali objectives, stating from the lowest: power objective 1 higher-power objective. 2. Before engaging the immersion objective in the fight path place @ crop ‘ofimmersion ol, provided with the 100x objective set, onto the specimen, {Rotate the revolving nosepiece to engage the Immersion objective and tum the fine adjustment knob to bring the specimen into focus. “Since air bubbles in the oil will affect the Image quality, make sure that the oil is free of bubbles, “To remove bubbles, rotate the revolving nosepiece slightly to move the objective in the oll by one reciprocation or wo. ‘oth the 125 condenser, the rated numerical aperture (NAY is the value ‘when oll is placed between the slice glass and the top lens of condenser. Foils not attached there, the NA is about 02. 4. Aer use, femave oll rom the cbjectve front lens by wiping with gauze ‘lightly moistened with absolute alcohol. [Link] of immersion oil iFimmersion oil enters your eyes or dntacts your skin, immediately take the folowing treatment Eyes: Rinse with fresh water (lor 15 minutes or more) king Rise with water and soap. ‘tthe appearance ofthe eyes or skins altered or pain persists, immediately ‘see your doctorCX31-P POLARIZED LIGHT OBSERVATION ‘As the microscope cannot manifest full performance in polarized light observation if the optical adjustments are not perfect, be sure to perform the following adjustments before absenation, Disengage the specimen, quarter wave plato, Sensitive tnt plate, tc. rom the light path betore proceeding © Cross-Nicol Adjustment | Gigs 21 82) 1. Set the Bertrand’s lens engaging/focusing knob @ to © (out to disen- ‘gage the Bertrand lens from the light path, 2. Loosen the analyzer clamping knob ®, push in the analyzer engaging! rotation knob ® and then tum itto align "0" ofthe rotary scale ® with °" [Link] the clamping knob 4, Slide the polarizer rotation knob © slightly in the horizontal ection to make the field of view darkest. Fig. 22 Polarization light oscillation direction adjustment + When the U-CBIS0-2 and U-CTR30-2 observation tubes is used, the cross lines rotate during the Interpuplllary distance adjustment so the following adjustment becomes meaningless. ‘@When its required to align the cross fines in an eyepiece with the polarized ight oscillation direction, use the oriented late (U-R) forthe adjustment ‘Orientation Plate (U-Pd) 1, Place the orientation plate on the stage, disengage the analyzer from the light path and adjust the focus using 4 low power objective . 15[Link] the center of the relerence plane of the orientation plate with the cross lines of the eyepiece, and engage the analyzer in the light path to achieve the cross-Nicol condition. 8, While obsening the ortented plate, rotate the stage til the orentaion plate looks darkest, anc clamp the stage rotation there Frotate the stage 50 thatthe bottom edge af the dark orientation plate is adjacent to the cross line, 4, Disengage the analyzer ftom the light path for the bright, and loosen the observation tube clamping screw slighty. 5. Rotate the observation tube so that the cross line in the eyepiece Is in paralle! withthe bottom edge of the orientation plate. and tighten the observation tube clamping screw, contention ross nes in eyeniene plate * eS ° a * = Reterenc ine Centering the Rotary Stage (gs 23 & 24) ; , C © Fig. 23 ¥ ‘© vvhen using the U-CTAD centering aciapter forobjectie lens, perform the operation in“ @| Adjusting the Centering Adapter for Objective Lens" on the next page before proceeding tothe folowing steos. When the U-CTAD centering adapter for objective lens is not used, ‘witching the objective may deviates the rotation center ofthe rota stage fom the center ofthe field of view I stct alignment is requied, adjust centeting everytime after switching the objective. 4. Place he specimen on the stage [Link] a mark @ on the specimen and ring that tothe center of cross lines in the cross eyepiece. 3. Loosen the stage rotation clamping knob @ tate the stage and tum the two stage centering knobs @ to bring the center ofthe vital cic} @, drawn by the otaéon ofthe stage, to the center ofthe cross lines 4 Move the specimen alone and bring @ second mark tothe center ofthe] 06s ines Repeat steps 9 and 4 a few times unt the enter of stage rotation ‘oincides with the center ofthe eyepiece cross lines. 16 &CX31-P ‘Adjusting the Centering Adapter cnn # pe cewen © This adjustment bings the center of conoscopic image accurately on the center ofthe feld of view It also ensures the rotation center ofthe ‘tary stage to be maiched perecty after objective switching, The accuracy ofthe centering adapter for objective lens is guaran- toed using the fixed combination of mount © and centering seat ® by giving the same identification numbers. Do not change the combination. (Example: 1 and U-CTAD1) 1 Remove the specimen and engage the objectne tobe usedin conoscopic cbsenation (one of 10x to 40x objectives) in the lght path. 2.70 sel the reference light ais, engage the analyzer in the ight path and seto the cross-Nicolconaiton. 4 Set the Bettands lens engaging/ocusing knob to the @ (IN) postion and observe the conoscopic image To facitate viewing of conoscopic image by brightening i rotate the analyzer sighty tom the coss-Nicol position 4 Inset the two centering knobs @ provided with the centering adapter for jective ens @ into the two centering holes and tum the knobs 80 that ‘he center ofthe conoscopic image comes on the center ofthe cross Ines. 5. Set he Bertrand lens engaging/tocusing knob to tne © (OUT poston and cisengage the analyzer rom the ight path [Link] the specimen on the stage and perm the opertion in" Cen- tetng the Fay Stage 7. Engage an objective other than the reference objective: and, wthou centering the otary stage, tun only the centering knobs of the cen ‘ering adapter for objocins fons 60 that the rotation center coincides with the center of feld even when the rotary stage is rotated. 17‘Use an objective between 4x to 100K. 1. When the UPA intermediate tube for polarizing observation is used, set the Bertand's lens engaging/focusing knob to the (© (OUT postion to disengage the Bertranc's lens from the Iight path. 2. Engage the analyzer in the light path and start observation. (Cross-Nicol position) if open-Nicol condition is require, disengage the analyzer from the light path. if pare-Nicol condition is required, set the analyzer to the 90° postion 3 Rotate the stage to set the observation target postion ofthe specimen dark (off postion) and then rotate the stage by 45* from there fo se to the dlagonal postion. The retardation (F) value should be measured in this postion. 4, The test plate (UTP 137 quatterwvave plate, U-TPS20 sensitive tint plate) Is used to produce sensitive colors and inserted in the test plate slot. Push the plat all he way into the slot to engage the plate in the light path, and put itil the clickstop positon to disengage it rom the light path For the usage of other compensators, refer to their instruction manuals. When the U-CWE quartz wedge is used, use the U-CWE2 that has the U-PA indication on the retardation reference Indication ®, {Use an objective between 200 100 1. Engage the analyzer in the light path and set to the cross-Nicol position. © 2:When the U-PA intermedia tube fr potatizing obsevaton is used, sot the Betrands lens engeging/ocusing knob to the @ CN) poston to engage the Bertrand’ lenin he laht path. 3. Engage an objective between 20x to 100x in the light path. [Link] the aperurischaphragm. 5. Tum the Bertrand’s engaging/focusing knob to focus on the conoscopic image as accurately as possible. When the U-KPA or U-OPA iniermedate tube is used. remove an eye- £008 from the sleeve end ook through ito view he conoscopi image. Fig 28 (©The image contrast may be improved by placing the interference fiter {45IF546) on the fitter holder on the base of the microscope. Ifthe peripheral pat ofthe conoscopic image is dark, move the con- ‘denser up and down to find the height at which the peripheral part is bites.‘tom ‘Specifications Optical system UIS2/UIS (Universal Infinity 8ysterr) optical system (infinity conection) Objectives Polarized light objectives ofthe PlanN&X:P and AchNYOXP to 100x0-° Eyepieces WHNTOX, WHNIO-, CROSS WHNOX | WHBI0X, WHBIOX Field number 20 Field number 22. Micrometer disk can be inserted. Micrometer disk can be inserted. Obsewaton tube | Binocular ube (U-BISOP. FN 22 Interpupilary distance |” U-CBI502: FN20 Interpupiay distance adjustment range 50 to 76 men, adjustment range 48 10 75 mm. Tinocular tube (UTTRSO-2 FIN 22 Light spitrato(Bin- | U-CTR30-2: FN 20. Light spilt ratio ocular: Straight) = 100:0, 20:80, | (Binocular: Straight) fixed at 50:50, :1001nierpupilary distance adjust-| Interpupillary distance adjustment ment range 50 to 76 mm, range 48 to 75 mm, Intermediate tube for | Berand’s lens Focusing possible polarizing obsewation er Orthoscopy/ ‘According to Bertrand lens engagement Knob on the right postion: @ 4 conoscopy switching | Knob on the let position: © OUT. ‘Analyzer Built in. Engagement/élsengagement possible. 160" rotatable. Clamping at desired postion possible. Scale with 90 divisions {2° minimum graduations, readout down to 6 possible by using the veriet) ‘Test plate, compen- | Sensitive tint plate (U-TP530), quarter-wave plate (UTP137) and various sator slat compensators are mountable Microscope tame | Light source (6,30 Whalogen bulb Preo=ntering/prefocusing system with felis dlaptragrn Builtin power supply: 100-120/220-240 V~., OB5/O45 A 50/60 Hz Condenser ‘Santee condense for polarized ight Top lens fixed. Polarizer 360° rotatable and detachable. NA 03 (1.25 when Immersed in oill. Aperture Iris dia- phragm #15 to $29, lever system tage Rotary stage for polarized light, with 2-point centering mechanism, 60" hhatizontal rotation can be clamped at a desired postion. 360° scale provided (1° minimum graduations, readout down to 6 possible by using the vemies, © Auxiliary specimen holder (provided) can be mounted, © Mechanical stage with cross movements (U-FMP) (optional can be mounted. Revowving ‘Quadruple postions, arm fixed, inner oriented nosepieos © Centering adapter for objective lens (U-CTAD) (optional) can be mounted, Focusing Siage height adjustment using roller guides (ack & pinion] Coarse adjustment mechanism stoke: 368 mm per tun. ‘otal stroke: 25 mm. Prevocusing knob provided, coarse adjustment knob rotation tension adjustable. Fine adjustment knob with 25 um graduated scale Operating environment indoor use ‘+ Alitude: Max 2000 meters ‘Ambient temperature: 6 to 40-0 (to 104° F) + Maximum relative humidity: 80% for temperatures up to S1°C (8B*P). de creasing linearly through 70% at 34°C (83°F, 60% at 87°C (89°F, to 60% relaive humidity at 40-0 (1047 + Supoly wotage Muctuations: +109. + Poluiion degree: 2 (in accordance with IECE0664) + nstallabor/Ovenvottage category In accordance ith 19OPTICAL CHARACTERISTICS = UIS series objectives not listed here can also be combined with this microscope. ~ ‘The following table shows the optical characteristics of ——1_ tect ype Seana ent au cess Te Hse = Fra ee a canoeannon Marion | na ae [motown mocneches tm Mechanical tube length +=" Field number (FN) Car vant / SS Cover ass thickness ‘Optical Characteristics Eyepieces cover Gis maantt-| ayy | WO |‘Rinas|Bsstion| _—__WHNIOX(EN 22) _| omar cation (mm) (um) (nm) ‘Total [FcalDecth] Field of Objectives: mag. | (um) | View PleniN-P Pian Achromat for 7 Paated Wt BN 20 ax | om | 15 | - | 396 | 40x | 1900 | 55 FaiNPhavomalforpoaized | ox | oz | 60 | - | 134 | fox | 280 | 22 light N 22), zx | ao | 30 | or | 084 | 20x | 609) ox | 065 | 45 | o17 | 052 | 400K | 304 | 055 sooxo | 125 | 013 | - | 027 | 1000x | _a69 | 022 formes Legend Working distance AND): Distance between the top surface of cover glass and the objective font lens. Numerical aperture (NAP The gute coresponding othe Frumber ofthe camera This is associated wth the esol tion and larger NA means higher resolution. Resolution: Abily of an objective for identlying adjacent te tines i the image, which is expressed in tems ofthe minimum distance between two points on the specimen surface. Focal depthvObjectskdéh The depth range of a specimen, in which focusing is obtained a atime. Stopping down the aperture ie diaphragm increases the focal depth and increasing the objective NA deoraases it Fiekd number FN) ‘The olameter ofthe image observed through an eyepiece, represented in milimeters, Actual field of view: Diameter ofthe fied of view, expressed as the size on the specimen surface. “eal power otal magritcaton: Product of Objective magnification x Eyepiece magnification. 20TROUBLESHOOTING GUIDE CX31-P Under certain conditions, performance of the mictoscope may be adversely alfected by factors other than defects. problems occur, please review the following list and take remiecal action as needed. Ifyou cannot sohve the problem after checking the entre lis, please contact Oympus for assistance. 44 Optical system 2) Thefleds darkeven whenthe lamp | The Berand’s lens is inthe ight path. | Disengage I fom the ight path 1 bulb ison The anavzer sett he coss-Ncol | Disengage the analyzer fom the ight | poston path 15) The field of view is cut off or not | The revolving nosepiece isnot setioa | Seti ito a cick postion, regulary iluminated click postion - The test plate is inserted midway. "The field is claphragm is eccentic._| Center i propery 8 The fel iis diaphragm is stopped | Open iio an optimum size a3 down tao much The objecive, eyenieces, condenser | Clean them thoroughly 2 and/or window lens ar city ©) Dust or stains are visible In obser | The window lens onthe base is diy. | Clean them thoroughly wind “The top lens ofthe condenser is diy. > “The specimen Is diy The eyepieces are diy «Observation image glares ‘The condensers too low. Raseit : 8 The apertures diaphragm s stopped | Open the aperture 3 «down too much, ©) Obsenvation image & poor “The objectne is nol engaged conecty | Sethe evoking nasepieoe conecly | —_ +The image i no! shar inthe light path into click postion +The contrast i low. The objective ont lens Is city Giean thorough * Deals ae solid and unclear, |e CDRANE TOTNES Cy. ay 2 Immersion ol is not used with an in | Use immersion ol ul mersion objective ‘Bubbles fe miedinihe immersion ol | Remove the bubbios ni The specfed immersion ais nolused | Useite immersion ol supped by Ofmons | 14 [The specimen is ity Giean them thoroughiy 2 The eyepaces and/or condenser are iy 7] Part of image is detocused or im |The objecive is not propery engaged | Sat the revohing noseplece conectly age looks like its towing, inthe ight path into a cick positon The specimen IS rot Set propery on | Sete specimen consclvonthestage | yp the stage. and secure using the specimen holder {@) The cross/icot condition cannot |The analyzer 1s disengaged tom the | Engage it inthe ight path, i be obtained light pat 1) The conoscopic Image cannot be | The Bertand’ lens sdsengaged tom | Engage tin the ight path 1 observed the lightpath 21‘2. Foous adjustment mechanism i a) The tension of coarse adjustment ] The coarse adjustment knob rolation | Loosen the ring to adjust to proper |g rab it too high tension adjustment ng is setto0 taht. | tension, Dy The sage lowers by Is own weIGHT | The coarse adjustment Knob rotation | Tighten the rng to adjust to proper crfocusing is lost due to siopage | tension adjustment ring is set too | tension, ofthe knob. loose. ©) Coarse focus adjustment cannot | The pre-focusing knob limits the stage | Raise the limiting position of thepre- |g raise the stage high enough, _| ata low poston focusing knob. @ Coarse focus adjustment cannot | The condenser holder's oo low. —_| Raise the condenser holder ° Tower the stage low enough @ Objecve his the specimen below | The specimen is upside down Sel the specimen conecty ~ accurate focusing is obtained 4. Binocular observation tube 42) Fields of view of two eyes do not | The inierpupilary dstance is not ad- | Adjust propery. 9 match. justed property, ~ Diopter compensation forthe two eyes | Adjust conecty 3 s not set “The i and right evepieces are di | Do nol stare athe image Immediately ferent ator placing your eyes on the eye- pieces. Fist take a look at the enti fold of iew. Removing youreyes once | ~ from the eyepieoes and placing them again may also work, 4. Stage a) Folating the stage renders the | The lay stage or centering adapter ] Center them. ‘wir ‘specimen invisible. for objective lens is not centered. Hl 5, Objective switching mechanism ‘ ¥ bs '2) Objective hits the specimen when | The specimen is upside down, Sot the specimen conrecty. - an objective is switched toa higher @ cover glass is oo thick ‘cover glass with thickness of salaried “Te cover glass i oo thi Us ass with tiekness of | a7 mm 6, Electrical system 7 a) Lamp bub does not light ‘Lamp bulb is not mounted ‘Attach a bulb 24 ‘amp bulb is blown Replace the bulb. 2 The power cord is unplugged Plug it securely 28 D) Lamp bulb blows easly “The speclied bulb isnot used Peplace wih a designated bulb 2a 22 4CX31-P YLT DG ‘The diagram below shows the sequence of assembly of the various modules. The numbers indicate the order of assemby, ‘The module model numbers shown in the following diagram are merely the typical examples For the modules with which the model numbers are not given, please consul the latest catalogues or Olympus, ++When assembling the microscope, make sure that all parts are free of dust and di, and avold scratching any parts or touching glass surfaces. ‘Assembly steps enclosed in. @Jj will be detailed on the subsequent pages. Teolto be uses Snore De Gpseryaton tube ‘Alen wrench provided WHNIOXHt AI UTRI0-2 with the microscope Cchoss wiavioxs uesge2 wrsox verRs0-2 tox For UBIGOP/UTRI02. Te + Sensitive tint plate U-TPEGO * Quarter-wave plate U-TP137 *Compensator U-CBE, U-CTB, U-CARI, U-CBR2, LOWE? (U-CWE}, U-CSE (Note) The U-CWE cannot be used for the UPA, {8 V, 30 W halogen bull 23Installing/Replacing the Lamp Bulb (Fig. 29) 1. Place the microscope frame on the back and pul the lock knob ® on the bottom to open the lamp bulb replacement cover 2, Hold the halogen lamp bulb @ without taking it out of the polyethylene. bag so as not to stain the bulb with fingerprints or stains, and push the ‘bulb all the way into the pin holes on the socket ® Alter ettaching, re- move the polyethylene bag from i Applicable lamp bulb> '6V, 30 W halogen bulb: 6VSOWHAL (Philips Type 5761) Always use the designated bulb. Using a bulb other than a specified ‘one may lead to a fre hazard. ‘A Fingerprints or stains on the lamp bull reduce its service life, When itis contaminated, wipe with a cloth slighty moistened with alcohol. ‘3. With the lock kno® lett in the pullec-out postion, close the lamp bulb replacement cover Then push in the lock knob to lock the cover The cover cannot be closed if the lock knob is in the pushed-in position. Make sure that it s in the pulled-out position before clos: Ing the cover. Fig. 28 Caution for Bulo Replacement During Use or Right After Use ‘AvThe bulb, lamp socket and areas near these will be extremely hot during and right after use. Set the main switch to *O" (OFF). dis rect the power cord from the wall outlet, then allow the old bull andl lamp socket to cool before replacing the bulb with a new bulb ofthe) designated type. ‘+ When replacing the bulb that is blown in the middie of observation remove the parts that may drop such as the eyepieces,fiter an« ‘specimen from the microscope frame, and tit down the microscops frame by 90°. ‘Attaching the Condenser (Fe.30) 1. Rotate the coarse adjustment knob to rise the stage tothe upper imi “Then, rotate the condenser height adjustment knob @ to lower the con} denser holder @ slight and then loosen the condenser clamping kno ©. 2. Insert the condenser @ into the condenser holder, Set the condenser ‘aperture scale to the front position and tighten its clamping screw. Rotate the condenser height adjustment knob to move the condense) holder to the upper limit 24CX31-P 3 | Mounting the U-PA Intermediate Fa.31) ‘Tube for Polarizing Observation the mounting method of other intermediate tube models, eferto their instruction manuals, ly basen the observation tube clamping screw © of the microscope frame using the Alen wrench provided with it [Link] the circular dovetail at the bottom of the intermediate tube for polarizing observation @ into the observation tube mount by aligning the positioning index * @ with the clamping screw position ® an tighten the clamping screw ©, Molinting the Observation Tube (o.31) 1. Fully loosen the observation tune clamping screw @ ofthe intermediate tube for polarizing observation ® using the Allen wench, [Link] the croular dovetal at the bottom of the observation tube into the observation tube mount of the attachment, bring the binocular tube at the operator's position and tighten the clamping screw ®, Mounting the U-CTAD Centeri KB Adapter for Objective Lens NO o2.524.99) +The accuracy of the centering adapter for objective lens Is guaran- teed using the fixed combination of mount © and centering seat @ by giving the same identification numbers. Do not change the combination. ‘Also do not use the unit in combination with another centering adapter for objective lens. Using the centering knobs @ provided with the centering adapter for objective lens, loosen the two screws ® clamping the mount © and centering seat @ to separate them. 2. Screw the mount @ fimily inte the revolving nosepiece, * 2 Insert the centering seat having the same idenifcation number as the ‘mount attached tothe revolving nosepiece, and tighten the two clamping ‘screws @.At this time, set the combination so that both ofthe twa center ing holes @ are uniformly visible from the front. Othenwise, it would be impossible to attach another centering seat. °26 Fig. 36 Fa. 37 ‘Attaching the Cord Hanger (CH3-CH) (Figs 34 & 35), ‘When the CH2-CH cord hanger is attached on the back of the micro- scope frame, the power cord can be wound around the cord hanger for storage. Engage the hook @ of the cord hanger © into the air vent groove on the: back of the microscope frame by aligning the mount postion, apply the ‘cord hanger to bring It in close contact withthe microscope frame and then slide the cord hanger down to fx ‘When carrying the microscope, do not hold it by the cord hanger. ‘Otherwise, the cord hanger would detach and cause the microscope to drop, which may also cause injury Removal “To prevent elactic shock, disconnect the power cord in advance and do not use a thin Allen wrench but always use the provided Allen wrench. dsPlace the microscope frame at the edge of the desktop @, insert the ‘Allen wrench © into the bottom of the cord hanger ® , push the Allen wrench in directions [f] and [2] to move the cord hanger upward and remove it. ‘Connecting the Power Cord (Figs. 36 & 37) As The power cord is vulnerable when bent or twisted. Never subject it to excessive force. AMake sure that the main switch is set to "©" (OFF) before connect- ing the power cord. » duAlways use the power cord provided by Olympus. ifn power cord Is provided, please select the proper power cord by referring to the section “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruction manual. 4 Connect the power cord’s connector @ to connector @ fly. ‘[Link] sure to supply power from a grounded, 3-conductor power outlet using the proper power cord. f the power outlet Is not grounded property, Olympus can no longer warrant the electrical safety perfor- mance of the equipment. 2. Connect the power coms plug @ to 2 wall power outlet @,CX31-P li PROPER SELECTION OF THE POWER SUPPLY CORD no power suoply cox is provided, pleas * Catled Cord * below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment. elect the proper power supply cord forthe equipment by refering to" Speciications" and Specifications Voltage Rating 125V AC (lor 100-420V AC atea) of 250V AC (for 220-240V AC area) (Current Rating 64 minimum Temperature Rating 60°C minimum Length 805 m maximum Frings Configuration Grounding type attachment plug cap. Opposite terminates in mokled-on IEC con- figuration appliance coupling Table 1_Centified Cord ‘A power supply cord should be certified by one of he agencies listed in Table 1 , or comprised of cordage marked with an ‘agency marking per Table 1 or marked per Table 2 The ftings are to be marked with atleast one of agencies listed in Table 1, In case you are unable to buy locally in your country the power supply cord which is approved by one of the agencies mentioned in Table 1, please use replacements approved by any other equivalent and authorized agencies in your county. County | Agency | Somneation Country Agency Se pcerina | BAM ® |» Ma ® Assraia | SAA Vv sepa ies | OT usta ove GD | Netretanes | ema if Berium | cesec [remy — | nemo ® Goan | oA @ [= fx @ Denmak | DEMKO ® — | suen | seo © Friant | @® — | sweets | sev ® France ute “fngdom | Ba e9 Gemay | We Bua u © reine | SA Ss= Table 2_HAR Flexible Cord [APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS: Prado Enoseed Harmonia [ARernative Marking Utlizing| tion Marking (May be located on |Black-Red-Yelow Thread (Length ‘Approval Organization eketer inculetion oftotemat wir [F601 section in) ing) Black | Red | Yellow Comite Elecrotechnique Beige 5 “ars a paula ceBec Har 10 x 10 ‘Verband Deutscher Elektrotechniker ue (VDE) eV Pritsicte 0B a8 Ed 0 0 Union Technique de TElecicie a uae 1} % un use HAR 20 0 institut talano del Marchio di ‘eve ae ENMEQU GHAR 10 x Batish Approvals Senice for Electic wae Cables BASEC) nary 7 > # [Link], KEMAKEUR HARD 10 20 20 SEMKO AB Svenska Elektiska ‘ cua Matesiclontolanstalter SeMKO one ° 0 9) Ostereichischer Verband fir = uae 0 5 Elearotochnik CVE) oe a * Danmaris Ellarske Materakontl ; ; pane OEMKO Ae 30 0 30 National Standards Authory of eland ‘NSA ans a (SAD ‘NSAP Norges Elltske Matetkontoll wr 0 7 Noes E NEMKO HA 19 ‘Asociacion Elecrotzenica Y- = an F 70 Electonica Espanola (AEE). ENED ” 0 Hellenic Organization for ‘wh 3 0 0 Standardization (ELOT) BLOT Instiuto Forages da Guaidade op wa 7 0 ~ Pa) Schweizerischer Elektro uae 0 0 oo ‘Tachnischer Verein SEV) sev Elektiske Inspektoratet sen eur 10 30 %0 Undenwiters Laboratories inc. (UL) Canadian Standards Association (OSA) SV,SVT,SJ or SIT, 3 X TBAWG SV,SVT, SJ or ST, 3 X 18AWG 28MEMOOLYMPUS Manufactured by OLYMPUS CORPORATION Shiv Monolith, 3-4, Ns Shika 2-home, Shinar, Tobe, Japan Distributed by.” =]=) OLYMPUS EUROPA HOLDING GMBH Wendenstrasse 14-18, 20097 Hamburg, Germany AMERICA INC. ‘3500 Corporate Parkivay, Center Valley, Pennsylvania 18034-0610, U.S.A. IPUS PT OLYMI E LTD. 491B Fiver Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373 LYMPUS RALIA PTY. LTD. 31 Gilby Road, Mount Waverley, VIC., 3149, Melbourne, Australia ob LATIN AMERICA, INC. ‘5301 Blue Lagoon Drive, Suite 290 Miami, FL'33126, U.S.A. AXT349 04 Printed in Japan 20141107 40200