Scribd Logo
Upload

Welcome to Scribd!

  • Gce H3 2011

    Uploaded by

    swordskylark
    10 views6 pages
    GCE

    Original Title

    GCE H3 2011

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    GCE

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    10 views6 pages

    Gce H3 2011

    Uploaded by

    swordskylark
    GCE

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    of 6
    sivintii MINISTRY OF EDUCATION, SINGAPORE in collaboration with UNIVERSITY OF CAMBRIDGE LOCAL EXAMINATIONS SYNDICATE Goneral Certfcate of Education Advanced Lovel fore PROTEOMICS 9818/01 pavers Cctberiovenber 2011 hows mime ‘Addional Matera: Anawer Paper (Graph Paper READ THESE INSTRUCTIONS FIRST ‘wrt your Cente number, index umber ane nar onal the work you hand in Wis ark tue or lack pon on bath sees of he paper You may ute a so pene or any diagrams or graphs Donatus stapes paper cps, righigters, gue oF correction fd. Section & “Answer al questions. ‘Section 8 ‘Anew thre ooh four questions. ‘Section € “Answer the queston, -Athe endo he exsnation, asten al your work secur together “The numberof marks gen in rackets [athe end of each question or part quostin. You may use a ecu ‘You re reminded ofthe ned fr clear presentation in your answers, “This document consists 6 printed pages and 2 bank pages. oe UNIVERSITY of CAMBRIDGE re hasnt om Invern Bento 0c (stay) 951859 [Turn over 1 2 Section A ‘Answer all questions in this section. Protoin-ipid interactions can be studied jn vito using liposomes, which minic cell membranes, Ina foosome-binding assay, protoins and liposomes are mixed, then ultacentrfuged at high ‘speed Proteins that do not bind liposomes remain in seluion, and proteins that bind liposomes are found in the preciptate Poured protein A was subjected to a fposome-binding assay, and analysed using SDS-PAGE, [A socond experiment with the same procedures was performed on protein A without the addition of liposomes. The resulting ge is shown in Fig 1.1 + Lanes 1 and 2 show the supernatant and precsitate, respectively from the liposome: binding experiment on protein A with liposomes added + Lanes 8 and 4 show the supernatant and precipitate from the experiment with no liposomes added + Lane 6 shows a molecular weight standard, with molecular weights written in klodalton ‘nits atthe side. 100 ®0 oo 0 Fig.1.1 (a) With roforence to Fig. 1.1, explain whether oF not the results show that protin A binds ligosomes, and the purpose of lanes 3, 4 and 5 a (©) Estimate the molecular weight of protein A. 1 (©) Aatforent protein, protein B, is a monomer in solution. When it binds tthe cell membrane, ikbecomes a cimer, Suggest @ possible function tor such a dimer, daring monomer an dimer in your anewor eet [age ppete ee fa] "ingleyston nut Sant igh (@) Namo and brie describe one mathod to tet whether or nat protein Ais a dimer in sokation, ©" Cetdehge M3 106 did wad LOGebw, Gem 2 Avworawide project involves collecting samples from diferent parts ofthe ocean and sequencing ‘any genomes present. This i called metagenomics. {8} Microorganism M (with genome M) is present in great quantity in the southern Pace Ocean, ‘Suggest how to identity this organism trom its genome, LOE FS) aot 660) RE ay (©) Ten open acing frames (ORFs) trom genome Mare transcribed and translate. owes Deseibe how homologues to these proteins could be foun, datning a homeogue in your as answer Losde a (©) Suggest how a project such as this metagenomics project can be used to help the ‘environment. 2 0°" (@)_ Explain wat 's meant by the term protoome and suggest how an understanding of the proteome can be beneficial to human health. Use a spectic example your answer. [3 2 Abnormal glycosylation of the dystrogiycan protein is a cause of one type of human muscular ‘ystrophy.A protein homologous fo human dyetroglycan protein has been fourd in mice (2) Name and describe a technique that could detect glycosylation of mouse dystagiycan protan, mss spee LoGeon Gown) a (©) In humans, he dystoglycan protein binds tothe dystrophin protein Doser the oe of binding sts in forming protein complexe, naming poten complex in your answer. LO +> (©) Name and describe two metheds upd to datermine the binding partners of mouse dystrogycan proton REE to out ta) {@) Suggest an experiment in mice 10 test whether introduction of the normal allele for

    You might also like