1. Staphylococcus aureus 8. Viridans Strep.

a. Gram + cocci in clusters a. Gram + cocci

b. Beta-hemolysis b. Alpha hemolysis
c. Catalase: + c. Catalase -
d. Coagulase: + d. Optochin resistant
i. Free (slide) and bound (tube) e. BE -
f. Salt tolerance: -
e. NO novobiocin or bacitracin
9. Enterococcus faecalis
f. Mannitol fermenter (yellow on MSA)
a. Gram cocci
2. Staphylococcus saprophyticus b. Hemolysis: gamma (sometimes alpha)
a. Gram + cocci in clusters c. Catalase -
b. Gamma hemolysis d. Salt tolerance: +
c. Catalase: + e. BE: + (black precipitate)
d. Coagulase: - f. PYR +
e. Novobiocin resistant (<16 mm) g. Optochin resistant
f. Bacitracin resistant (no zone) h. Lancefield group D
3. Staphylococcus epidermidis 10. Streptococcus bovis
a. Gram + cocci in cluster a. Gram + cocci
b. Gamma hemolysis b. Hemolysis: gamma, sometimes alpha
c. Lancefield Group D
c. Catalase: +
d. Salt tolerance -
d. Coagulase: -
e. BE +
e. Novoboicin sensitive (>16 mm)
11. Corynebacterium spp.
f. Bacitracin Resistant a. 370C in a 5% carbon dioxide-enriched
g. Mannitol non-fermenter but has growth atmosphere
4. Micrococcus spp. b. Gram + rod (pleomorphic)
a. Gram + cocci in tetrads c. Catalase +
b. Gamma hemolysis d. Nitrate +
c. Catalase: + e. Glucose fermenter
d. Bacitracin sensitive (10.5-25.0 mm) f. Urease
5. Streptococcus pneumonia g. Non-motile
a. Gram + lancet shaped diplococci h. Non-spore forming
b. alpha hemolysis 12. Bacillus Spp. (Ours is motile)
c. Catalase - a. Gram + bacilli
d. Optochin (P disk) sensitive b. Large, spreading, gray colonies with
6. Streptococcus pyogenes irregular edges
a. Gram + cocci in chain c. Endospores
b. Beta hemolysis d. B. anthracis
c. Catalase - i. Gamma hemolysis
d. BE - ii. Non-motile
e. PYR positive but dont test iii. Catalase +
f. Lancefield group A e. B. cereus
g. CAMP - i. Beta hemolysis
h. Bacitracin sensitive (presumptively IDs) ii. Motile
7. Streptococcus agalactiae iii. Catalase +
a. Gram + cocci 13. Listeria monocytogenes
b. Beta hemolysis a. best at neutral to slightly alkaline pH
c. Catalase - b. Gram + bacillus
d. PYR - c. Beta hemolysis
e. Lancefield group B d. Tumbling motility
f. CAMP + e. Catalase +
f. Non-spore forming
g. BE positive (small black colonies)
14. Erysipelothrix rhusiopathiae c. Non-hemolytic
a. Gram + small short rod d. Requires X factor
b. Alpha hemolysis (after prolonged e. H. ID Quad: growth on I & III
incubation)
21. Escherichia coli
c. Catalase
d. Non-motile a. Gram - rod
e. TSI: K/K (red/red) with H2S production b. Oxidase -
15. Neisseria gonorrheae, c. TSI: A/A Gas (yellow/yellow, cracks)
a. Grows best in CO2 d. MAC: LF (pink with darker pink
b. Gram diplococci surrounding)
c. Oxidase + e. HE: Yellow (lactose fermenter)
d. MTM: growth f. API
e. CTA: only glucose + 22. Citrobacter freundii
f. No growth on BAP a. Gram - rod
16. Neisseria meningitides b. Oxidase -
a. Grows best in CO2 c. TSI: A/A gas H2S, (yellow/yellow, cracks)
b. Gram diplococci d. MAC: Late LF (pink)
c. Oxidase + e. HE: Red, yellow, or colorless
d. MTM: growth f. API
e. CTA: Glucose and Maltose + 23. Klebsiella pneumoniae/oxytoca
17. Moraxella catarrhalis a. Gram - rod
a. Growth stimulated by CO2 b. Oxidase -
b. Gram diplococci c. TSI: K/A (red/yellow)
c. Grows on BAP and chocolate d. MAC: LF, mucoid
d. MTM: growth e. HE: yellow
e. CTA: all negative f. API
f. Colony can be pushed around like 24. Proteus mirabilis/vulgaris
hockey puck a. Gram - rod
18. Haemophilus influenza b. Oxidase -
a. Growth stimulated by CO2, grows only c. TSI: A/K, (yellow/red)
on Choc agar d. MAC: NLF, swarming
b. Gram rod e. HE: Colorless
c. Oxidase + f. API
d. Non-hemolytic on BAP 25. Salmonella spp.
e. Requires X and V factor a. Gram - rod
f. H. ID Quad: growth on III b. Oxidase -
19. Haemophilus parainfluenzae c. TSI: K/A, Gas, H2S + (red/yellow, cracks,
a. Growth stimulated by CO2, grows only black)
on Choc agar d. MAC: NLF (clear)
b. Gram rod e. HE: green, black
c. Oxidase + f. API
d. Non-hemolytic on BAP 26. Serratia marcescens
e. Requires V factor a. Gram rod, red pigment
f. H. ID Quad: growth on II and III b. Oxidase -
20. Haemophilus ducreyi c. TSI: K/A (red/yellow)
a. Gram rod, school of fish d. MAC: Colorless, mucoid
b. Oxidase + e. API
27. Acinetobacter baumanii
a. Gram rod, resist decolorization
b. Oxidase -
c. MAC: NLF, can appear purple
d. API
28. Stenotrophomonas maltophilia
a. Gram rod (short to medium)
b. Oxidase -
c. MAC: NLF (clear)
d. Catalase +
e. API
29. Pseudomonas aeruginosa
a. Gram rod, straight and slender
b. Oxidase +
c. MAC: NLF
d. TSI: K/K (red/red)
e. API
30. Aeromonas hydrophila
a. Gram - rod
b. Oxidase +
c. MAC: Both LF and NLF
d. API
31. Alcaligenes xylosoxidans
a. Gram - rod
b. Oxidase +
c. MAC: NLF
d. API