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APPARATUS
1. Acetic Acid - chloroform solution (480ml Acetic Acid and 320ml Chloroform).
2. Saturated Potassium Iodide solution. Store in the dark.
3. Sodium thiosulfate solution, 0.1N. Commercially available.
4. 1% Starch solution. Commercially available.
5. Distilled or deionized water.
PROCEDURE
1. Weigh 5.00 (0.05)g of sample into a 250 ml glass stoppered Erlenmeyer flask. Record
weight to the nearest 0.01g.
3. Swirl the flask until the sample is completely dissolved (careful warming on a hot plate
may be necessary).
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2
PROCEDURE cont.
5. Stopper the flask and swirl the contents of the flask for exactly one minute.
8. If the starting color of the solution is deep red orange, titrate slowly with mixing until the
color lightens. If the solution is initially a light amber color, go to step 9.
10. Titrate until the blue gray color disappears in the aqueous (upper layer).
Note: for Doe-120, titrate until the lower layer has a "milky" appearance.
11. Accurately record the mls of titrant used to two decimal places.
CALCULATIONS
S = titration of sample
B= titration of blank
or
(S - B) X N thiosulfate X 200
PRECISION*