V-500 Series Operation Manual

4. Standard Measurement Program

4.1 [Quantitative Analysis]

Quantitative Analysis
Calibration curve
.

4.1.1 [File]
Quantitative analysis , , .

1) [New]
.

[New] Calibration Curve [Open

Parameters] .

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: Calibration curve .

: Calibration curve . [Method]-[New]

: Calibration curve .

: Calibration curve

: .

: Quantitative Analysis Method File .

2) [Open...]
.

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: .

3) [Save]
[Data Sheet] File Name . Measurement
Parameters Calibration Curve Data .
Data .

4) [Save As]
[Data Sheet] . Measurement Parameters Calibration
Curve Data .
[File]-[Save As] .

: .

: . JQA(Jasco

quantitative Analysis) .

File name list : .

: .

JQA : Jasco Quantitative Analysis

: Sample Name, Operator, Comment, Copyright

<Comment Edit> .

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: .

5) [Page Setup]
[Data Sheet], [Calibration Curve], [Parameters]
.

: . 62 32

: Method - Calibration curve .

Result - Quantitative Analysis .

: .

Data : .
Graph : Calibration curve .
Pattern result
.
Parameters : .
Comment etc, : Comment .

: Title .

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6) [Print Setup...]
Printer , , ,
.

: .

Printer Setup [ ]
Windows95

7) [Print...]
[Page Setup] .

(Tool bar .)

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4.1.2 [Method]
Quantitative Analysis Method , , .
[Method] .

1) [New...]
Calibration Curve .

[Quantitative Measurement - Parameters]

[New...] .

[Quantitative Measurement - Parameters] Calibration Curve

Standard() Parameter .

: Detector .

Response .

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: Slit Band width .

Model Band Width

[Method] : Calibration Curve Standard()

.

(1) 1-wavelength
A bs
Baseline correction
. 1-wavelength Peak
calibration
0
curve .
P eak

(2) 2-wavelength
Peak Base 1 A bs

Calibration curve
.
0
B ase 1 P eak

(3) 3-wavelength
Abs
Base 1 Base 2
baseline , baseline
standard Calibration Baseline
0
curve . 3-wavelength
Base 1 Peak Base 1
.

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: .

: Sample .

: Sample . 2 [Cycle

time] .

[Cycle Time] : . Cycle Time

.
Cycle Time : 0999sec

: Measurement Parameter [Calibrate Curve

Parameters] .

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[Calibrate Curve Parameters]

(Standard data ) : standard

calibrate data setting standard .
Standard data standard (line)
,
. standard calibrate data setting
calibrate data setting .

: Calibration curve .

Proportional Linear Quadratic Third-order

expression expression expression expression

Segmented Logistic Spline

line function function

:Standard measurement standard blank

. Standard measurement Blank

Blank 0.000 . Standard blank

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standard blank .

: Standard Blank

: Standard() Standard blank .

: Standard blank .

: Standard data standard()

.

: Standard data standard

: Standard measurement Standard

. 0.000 .

Standard .

: Standard data .

: standard data calibration curve .

(standard data [Use?] use .)

: standard data calibration curve

.
(standard data [use?] --- . )

: Calibrate Data Setting ,

, ,

standard data . Calibrate Data Setting

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: [Quantitative Measurement Parameters] .

[Quantitative Measurement Parameters]

response bandwidth .

: Calibration curve .

: standard [Quantitative Measurement]

[Quantitative Measurement]

: Standard() .

: Blank .

: .

: Standard data standard (

standard) ,
[Standard Data ] [Abs]

. [Use?] [---] [Use]

.

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Standard_00x standard_00(x+1)
.

: .

Blank Blank .

: [Quantitative Measurement] .

: [Goto Wavelength]

: .

: .

: 0 .

Autozero standard cell holder sample cell

holder blank cell .

2) [Open...]
Calibration curve .

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: Calibration curve .

: Calibration curve . [Method]-[New]

: Calibration curve .

: Calibration curve

: .

: quantitative analysis method file .

3) [Save As...]
Calibration curve .

[Save parameters]

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[Parameter Name] : calibration curve .

: Calibration curve .

: Sample name, operator, comment, copyright

<Comment> .

: .

4) [Modify...]
[Calibrate Curve Parameters] calibration curve .

5) [Information]
Calibration curve [Method's Information] .

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4.1.3 [Measurements]
[Measurements] [Data Sheet] .

1) [Measurement]
Calibration Curve .

: Sample () .

: Blank .

: .

: [Data sheet] .

: [Data sheet] Blank

Blank Sample .

: [Quantitative Measurement] .

: [ Goto Wavelength ]

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: .

: .

: 0 .

Autozero Standard cell holder Sample cell

holder Blank cell .

2) [Parameters...]
[Quantitative Measurement Parameters] . [Parameters...]
[Quantitative Measurement Parameters] Response Bandwidth
.

3) [Blank Correction]
Sample Blank Sample Blank .

: Blank . Sample Blank

.

: Sample Blank .
4.1.4 [Edit]

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1) [Copy Picture]
Calibration curve clipboard picture .
Copy picture calibration curve Microsoft draw power
point draw-type software text .

2) [Copy Bitmap]
Calibration curve clipboard .

Copy bitmap calibration curve paintbrush print type

software .
Bitmap ,

.
[Copy Picture] [Copy Bitmap] calibration curve
[] .

3) [Copy Text]
Quantitative analysis data sheet clipboard .
[Copy Text] [Data sheet] .

4) [Invalid]
Data sheet data . Data sheet data
[Invalid] Data . Data sheet
data [Copy text] .

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5) [Data Input]
Data . .

Data <Ok> Data .

6) [Title...]
.

<OK> .

4.1.5 [View]
[View] [Data Sheet] [Calibrate Curve] ,

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1) [Font...]
[Data Sheet] [Calibrate Curve] .
[Calibrate Curve] [] [Font]
.

[Item] : [Calibrate Curve] Axis Label, Scale Label

[Vertical Label Orientation] : Vertical Label

.
:
:

: .
( Calibration curve Font
.)

: [] [Item]

Data sheet [Font]

[] .

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2) [Format...]
Data Sheet Data .

International number list : .

3) [Cell Width...]
Data Sheet .

: .

: 16 .

4) [Scale...]
Calibrate Curve .

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5) [Pattern]
Calibration Curve Frame, Grid Line , , .

: Calibration curve, Frame, Grid Lines ,

, .
Calibration curve : Calibration curve .
Grid Line : Calibration curve .
Frame : Calibration curve .

[Color] : [Element] List .

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[Line Style] : [Element] List .

[Line Width] : [Element] List .

( [Line Style]
.)

: .

: .
( Calibration curve Pattern
.)

6) [Grid...]
Calibration curve Grid Line .
Grid Line Calibration curve

Calibration curve Main Grid () Auxial Grid ()

. Main Grid Line Grid Line Auxial Grid Line Main
Grid Line .
Grid Line , , [View]-[Pattern] Grid Line
[View]- [Style...] .

[Main : ] : Main grid( ) .

[Main : ] : Main grid( ) .

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[Auxial : ] : Auxial grid( ) .

[Auxial : ] : Auxial grid( ) .

: .
( Grid Grid .)

7) [Style...]
Grid Scale Label .

: Grid Axis Label

. Abs(), Con().

[Interval] : (Grid) .

: .
: . [Manual]
Main Grid () Auxial Grid ()
.

: Scale Label .

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8) [Marker...]
Calibration curve Standard , ,
.

: Marker .

Color : Marker .

: Marker .

4.1.6 [Window]

1) Cascade 2) Tile

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4.1.7 [Help]
Quantitative Analysis Program .

4.2 [Spectrum Measurement]

[Spectrum Measurement] .

4.2.1 [Measurement]

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1) [Start]
[Start] [Spectrum Measurement]
.
spectra analysis program .
[Start] [Spectrum Measurement] <Start>
, <Start> <Stop> .
<Stop> , <Stop>
spectra analysis program .

2) [Parameter...]
Parameter [Spectrum Measurement-Parameter]
.
[Spectrum Measurement-Parameter] [Parameter]
[Data File] .

[Parameter]

: .

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: Detector .

Response .

: Slit Band width .

Model Band Width

1) V-570 NIR Visible NIR

Bandwidth Visible Bandwidth
. NIR Noise .

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: .

.
V-530 : 40, 100, 200, 400, 1000, 2000, 4000nm/min
V-550/560/570 : 10, 20, 40, 100, 200, 1000, 2000,
4000nm/min

: .

: .

( Start .)

: Data Data .

Data pitch .
V-530 : 0.1, 0.2, 0.5, 1, 2 nm
V-550/560/570 : 0.025, 0.05, 0.1, 0.2, 0.5, 1, 2nm

2) Data 1600 Data

pitch . Data pitch .

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3) Spectrophotometer Detector Response Quick 1 33

Signal . Detector signal
Scanning Detector Signal

. [Scanning Speed] [Data Pitch] .

[Scanning Speed] Data pitch .

: Sample . Parameter

Sample No. Sample No.

.

: . No. of Cycle 2

[Cycle Time] .

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: , . Auto

Abs 01, %T 0100 .

: Parameter .

[Data File]

[Spectrum Measurement-Parameters] Tag [Data File]

.

: .

: Auto Save .

: Auto Save .

: Auto Save Files

. File Name 5 , File name

.
.

) test001.jws, test002.jsw, test003.jws

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: Auto Save

[Parameter Save]

Parameter Parameter
.

[Spectrum Measurement-Parameters] [Parameter Save]

.

: Parameter . 32

Parameter .

: Parameter File [Parameters Save] .

: Parameter List Parameter .

: Parameter .

[Parameter Open]
Parameter .

[ Measurement-Parameter] [Parameters
Open] .

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[Parameter List] : Parameter .

: Parameter .

: Parameter .

: Parameter .

3) [Baseline]
Baseline Correction .

: Memory Baseline .

: Baseline Memory .

: Memory Baseline .

: Baseline .
: Memory Baseline
.

: Baseline .

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[Baseline measurement] .

: . Baseline Memory .

4) [Move Wavelength...]
.

: .

: .

5) [Auto Zero]
0 Abs 100%T .

6) [Exit]
Spectrum Measurement .

4.2.2 [Help]

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1) [About...]
Spectrum measurement .

4.3 [Time Course Measurement]

.

4.3.1 [Measurement]

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1) [Start]
<Start> Time Course Measurement
.
Spectra Analysis Program .
<Stop> , <Stop>
Spectra Analysis Program .

2) [Parameter...]
Parameter [Time Course Measurement-Parameter]
.
[Time Course Measurement-Parameter] [Parameter]
[Data File] .

[Parameter]

: .

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: Detector .

Response .

: Slit Band width .

Model Band Width

: .

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: .

: Data Data .

1) Data 1600 Data

pitch .

1600 ( End time Data pitch)

: Sample . Parameter

Sample No. Sample No.

.

: , . Auto

Abs 01, %T 0100 .

: Parameter Parameter .

[Data File]

[ Measurement-Parameters] Tag [Data File]

.

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: .

: Auto Save .

: Auto Save .

: Auto Save Files

. File Name 5 , File name

.
.
) test001.jws, test002.jsw, test003.jws

: Auto Save

.
[Parameter Save]

Parameter Parameter
.

[Spectrum Measurement-Parameters] [Parameter Save]

.

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: Parameter . 32

Parameter .

: Parameter File [Parameters Save] .

: Parameter List Parameter .

: Parameter .

[Parameter Open]
Parameter .

[ Measurement-Parameter] [Parameters
Open] .

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[Parameter List] :

Parameter .

: Parameter .

: Parameter .

: Parameter .

3) [Move Wavelength...]
.

: .

: .

4) [Auto Zero]
0 Abs 100%T .

5) [Exit]

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Time course Measurement .

4.3.2 [Help]

1) [About...]
Time course measurement .

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4.4 [Fixed Wavelength Measurement]

.

: Parameter .

: .

4.4.1 [Measurement ]

1) [Start]
Parameter .

2) [Parameter...]
Parameter [Fixed Wavelength Measurement]
.

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: .

: Detector .

Response .

: Slit Band width .

Model Band Width

: .

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: .

: .

: ( )

: ( ) .

: Sample . Parameter

Sample No. Sample No.

.

: . No. of Cycle 2

[Cycle Time] .

: Parameter . Parameter

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: Parameter .

: Parameter File [Parameters Save]

.

: Parameter List Parameter

.

: Parameter .

: Parameter .

[Parameter List] : Parameter .

: Parameter .

: Parameter .

: Parameter .

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3) [Move Wavelength...]
.

: .

: .

4) [Auto Zero]
0 Abs 100%T .

5) [Exit]
Fixed Wavelength Measurement .

4.4.2 [Data]

1) [New]
Fixed Wavelength Measurement .

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2) [Save As]
Fixed Wavelength Measurement .

Fixed Wavelength Measurement .

3) [Print]
Fixed Wavelength Measurement .

4) [Print setup]
[ ] .

4.4.3 [Help]

1) [About...]
Fixed Wavelength Measurement .

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4.5 [Abs/%T meter]

[Abs/%T Meter] Analog Spectrophotometer .

: .

: .

: .

: .

Data ,

: .

: .

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: .

: 0 Abs 100%T .

: Parameter .

: Detector .

Response .

: Slit Band width .

Model Band Width

: [Abs/%T meter] Version .

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4.6 [Environment]
Hardware , , Optional Accessory , Wavelength Correction
.
[Spectra Manager] [Environment] .

1) [Hardware Setting]
On/Off .

: Deuterium On/Off .

: ON
: Off

: Halogen On/Off .

: ON
: Off

: Deuterium Halogen .

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: 330nm 350nm

: UV/Vis Grating NIR Grating

.
: 750nm 900nm

: Deuterium .

: Halogen .

2) [Diagnosis]
.

3) [Accessories Setting]
Spectra manager .
.

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Optional Intelligent Accessories .

4) [Wavelength Correction]
Deuterium Lamp Wavelength .

[Wavelength Range] : Wavelength . V-570

NIR .

: Wavelength .

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