Sample: dow1 Rf 200 Friday 28 July 2017 01:36PM

RediSep Column: Silica 80g Peak Tube Volume: Max.

SN: E04102B2278E0 Lot: 2518205010W Non-Peak Tube Volume: Max.
Flow Rate: 30 ml/min Loading Type: Solid
Equilibration Volume: 287.5 ml Wavelength 1 (red): 254nm
Initial Waste: 0.0 ml Peak Width: 2 min
Air Purge: 1.0 min Threshold: 0.20 AU
Solvent A: hexane Wavelength 2 (purple): 280nm
Solvent B: ethyl acetate

Run Notes:

12 14 16 18 20 22 24 26 28 30 33
13 15 17 19 21 23 25 27 29 31

2.00 90
80
1.75
70
1.50
60
1.25
50
1.00
40
0.75
30
0.50 20
0.25 10
0.00 0

20.0
Absorbance Run Length 60.0 Min Percent B

Rack A Peak # Start Tube End Tube

70 69 68 67 66 1 A:15 A:18
2 A:19 A:31
61 62 63 64 65

60 59 58 57 56

51 52 53 54 55

50 49 48 47 46

41 42 43 44 45

40 39 38 37 36
Duration %B Solvent A Solvent B
31 32 33 34 35
0.0 0.0 hexane ethyl acetate
30 29 28 27 26 5.0 20.2 hexane ethyl acetate
54.4 20.2 hexane ethyl acetate
21 22 23 24 25 0.0 20.2 hexane ethyl acetate
0.0 20.2 hexane ethyl acetate
20 19 18 17 16
0.6 20.2 hexane ethyl acetate
11 12 13 14 15

10 9 8 7 6

1 2 3 4 5
18 mm x 150 mm Tubes
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