Pellet the cells and discard the supernatant.

Lyse the cells using lysis buffer (8M Urea, 2M Thiourea, 4% CHAPS). Gently
add the lysis buffer to the pellet with intermittent agitation. Check for lysis of
cells, if required perform short pulse sonication.
Chill good quality acetone in a -20 freezer. Prepare a solution of
acetone/water (4:1) and store at -20.
Add 4 volumes -20 acetone to a sample extract. Vortex well. Place sample at
-20 for 2 hours.
Spin at 13, 000 - 16,000 x g for 10 minutes at 0-4. Carefully pour off the
supernatant. Wash pellet twice with -20 acetone/water (4:1). During each
washing, make sure the pellet is well broken up. Spin each washing at 13,000
- 16,000 x g, 0-4, for 10 minutes.
Pour off final washing supernatant. SpeedVac samples for 10-15 minutes.
Dissolve in desired rehydration buffer.