4.

Marmes reagent:

[KI 20 gm. in 20 ml dist. water

CdCl2 10 gm. in 50 ml dist. water]

5. Dilute H2SO4:

[Dist. water 30 ml]

Chemical Tests for the Detection of Alkaloids:

Following Tests are performed with the Extract:

Experiment:

1. Mayers test:

To about 3 ml. of extract, a few drops of Mayers reagent are added.

2. Dragendorffs test:

To about 3 ml of extract, a few drops of Dragendorffs reagent are added.

3. Wagners test:

To about 3 ml of sample solution, a few drops of Wagners reagent are added.

4. Marmes test:

To about 3 ml of extract, a few drops of Marmes reagent are added. Then dil.
H2SO4 is added to the mixture.

Observations:

Precipitate is formed.

Brownish fluorescent precipitate is formed.

Brownish precipitate is formed.

White or yellow precipitate is formed. Precipitate may be dissolved on excess

addition of Marmes reagent or ethanol.

Inference:

Presence of alkaloid.

Presence of alkaloid.

Presence of alkaloid.

Presence of alkaloid.