Professional Documents
Culture Documents
Prepared for
Office of Nutrition
Bureau foi- Research and Development
U.S. Agency for International Development
Washington, D.C.
Contract No. DAN-5116-C-00-O114-00
Project No. 936-5116
ii
PREFACE
For a number of years, those involved with the food approach to combatting vitamin
A deficiency in developing countries have been pressing for more infcrmation on the
carotenoid content of foods This publication ,rings together data on as many
different foods as possible as long as the data met mnmniiim demands of quality It
does not include data recently compiled by the Nutrient Composition Laboratory of
the United States Department of Agriculture on foods consumed in the United States.
In the light of comnients and new data received, the manuscript was revised in the
period October-December 1993 Data received before 30 November was considered
for inclusion in the database
We wish to thank IUr Frances Davidson, Deputy Director of the Off!ce of Nutrition of
the Research and Development Bureau of the U.S. Agency for International
Development (USAID R&D/N), and Mr Robert Pratt and Ms Mary Linehan who
are responsible for managing the \/itamin A Field Report Project (VITAL) of
USAID R&D/N at tht International Science and Technology Institute Inc for their
help, advice and support throughout the project We wish to express our gratitude to
all of those people who supplied data and/or suggested amendments to the
manuscript In partIcular we would like to thank I)r Gary Beecher and Ms Joanne
Holden of the U S Department of Agriculture Nutrient Conposition Laboratory
(Beltsville, MD) for providing further information on the CAREX expert system for
evaluating carotenoid data, Professor David Southgate formerly of the Agricultural
and Food Research Council Instittte of Food Reseaich in Norwich (U.K ) for his
advice in applying the system to the types of data encountered, Dr John Klens~n of
the INFOODS Secretariate in Boston (U S A ) for assistance in designing the
database, and Ms Esther Reichman and Mis Lous ''uym in Wageningen for
assistance in entering the data and for secretarial support
iii
TABLE OF CONTENTS
Preface iii
1. Introduction I
2. Methods 3
Data evaluation 4
Introductory notes 7
Cereals 9
Dairy products 10
Eggs
i
Fats 12
Fish 13
Fruil. 15
Meat 61
Oils 62
Vegetables 64
7. References 193
Appendices
1. List of people providing information 201
V
1. INTRODUCTION
Up until now, programs to combat vitamin A deficiency have been directed largely
towards the use of vitamin A supplements and much less effort has been directed
towards using locally available foods Most people agree that the use of iich foods is
the method of choice for combatting vitamin A dcficiency in the long term The
reasons for this are that people can use resources available locally, and that
increasing intake of vitainin A-rich and provitamin A-rich foods will also increase the
intake of othei nutrients Neither of these conditions can be met by tile use of
synthetic preparations of vitamin A provided either as a vitamin A preparation per se
or in fortified foods
One of the limitations to developing strategies and designing programs for controlling
vitamin A deficiency using local foods, and for evaluating such endeavours has been
the lack of reliable inforiation on the provitamin A carotenoid content of foods In
addition to haM1ng such inforal,tion it is essential that, intile future, more attention
should be directed towards tie problem lcarotenoid bioavailability as it is known
that bioavailabrliitV can be reduced markedly by tile matri\ in which carotenoids are
found, tile fat intake O n1divildtlials, and the nature aid degree ot intestinal
parasitism Another liinitation to developiig ,trategies and designing prograns is the
lack of understanding ot the tactors intl ucncing food habits with re,,pect to the intake
of foods rich in v.tamin A and provitainin A carotenoids When riore inrformation is
available on present and realistic future intake of vitamin A and carotenes corrected
for their bioavailahiiity, it will be possible to determine to what exten, other measures
will need to be taken to incre,tse vitamin A intake of particular groups of various
populations
In recent years, much progress has been made in the development of methodology for
quantifying the content of inldividual carotenoids in foods based on high performance
liquid chromatogi aphy (IIPLC) Initially toods were first analyzed by rleasuring the
extinction of extracts of foods This method gave an omerestinate of the piovitamin A
content of foods as simple extracts 'ilso contain carotenoids which are incapable of
being converted to vitamin A It is possible to separate various classes of carotenoids
from the provitamiln A carotenoids, particularly those containing oxygen by open
column chromatography, and then to measure the extinction of the provitamin A
carotenoid ,raction This method has proved very useful inproviding information on
the provitamin A content of foods lowever, when the necessary infrastructure and
equipment are available, IIPLC is the method of choice since it provides data on
individual carotenoids This allows specific conversion factors for each carotenoid to
be applied for tie calculItioriof vitamin A activity it is also now realized that
carotenoids have activity independent of th'tt derivel by their conversion to vitamin
A. This activity is attributed largely, but not excluswely, to their ability to serve as
antioxidants
presented which enables data on the carotenoid content of foods derived by using
HPLC to be evaluated The present work does not include data from this earlier
paper but has examined data from other sources with special emphasis being given to
data for foods from developing countries It has also used CAREX as the basis for
the evaluation of data Since the system is based on data available on US foods, the
criteria can afford to be somewhat strict but there are very few data for foods from
developing countries which meet even the minimal standards of the CAREX system.
Therefore in the present work, criteria for inclusion were relaxed but the quality of
the data is indicated.
2. METHODS
A letter request iig thatiinformation oi the carote noid coelent of food, be returned
to the Project Oflice ,1,.seit ti )Thout 110 people throughout the v%
orld After follow
up arid the meetings at %lijch the draft as reviewed, 78 responses were received
In addition, a search of the literature was undertaken with particular attention being
given to the follf\ ing sotrccs
enific lWeiitt re
Scrci \ith spcil reference to the hIl( , ng key jou rnals !-ood
Cheriiistr\, Internatimoial Journal of Food Science aiid Iechncliogv, Journal of
Agricultii l ail I (iod ( heiiitry, Journall of ('hromaito graph, iurnal of F)od
Science, Iournal of I iqid Clhro matography, Journal of Food ( ornposition and
Analysis, hoirnal of Micrirlutrie nt AnilysiS, Iourn;a of 'ood Q.uality, and
Jou rnal (if tile Scie'ICe of lood anild Agriculture
National and regia lil looid compositi(n tables including tile regional databases
of INFOOI)S
Newsletters, niilliUte <, Of meeting', leatlet, and other grey literature of
organization , and institUltCs iterested inthe carotenoid content of foods
including Vlt,iniiin A Sieve, Xerophth;alrn a Club Bulletin, Carotenoid News,
VitAl News and Vitan~ln A News Notes
3
Data reported in existing food composition tables were carefully examined as they are
often not original analytical values but imputed, calculated or borrowed from other
sources This is particularly so for data in tables from developing Countries Only
original analytical values and values calculated from these were considered acceptable
for inclusion in the database Where possible, borrowed valIis were traced hack to
their source and, it appropriate, included lowever, many tables do not provide
adequate reference to the source of data A visit was made to the Nutrition Section of
the Ministry of Agriculture, Fisherie,. and Food in London in order to determine
those values in the United Kingdom food composition tables which were derived from
analyses by the UK Laboratory of the Government Chemist For other data, where
informaton iP the publishCd or unpublished material was limited, authors were asked
t, provide additional Ietails, uIsual ly on the analytical method used
Most attention has been given to the carotenoid content ot vegetables and fruits In
addition, there is some data hor meat, fish, fats, eggs, cereals and dairy products.
Since some of the variation in tile reported composition of foods may be attributable
to such factors as varietv, groing conditions and time of harvest, data have not been
aggregated to any great extent It is thought that thils l id make the dat'base more
useful to investigators throughout the ,\)rld Where data have been aggregated (for
example, for different varieties or time, of harvest), this has been denoted in the
database and in the table presented in Chapter 3 )ata for cooked foods have been
included when they meet the e alKnation criteria
Data evaluation
In order to evaluate the quality of the data reported in publications, it is necessary to
establish criteria Data quality depends to a large extent on sampling procedure and
analytical methodology Over the last two decades wth the introduction of high
performance liqu1id chromatography (IPLC), it has become easier to identify and
quantify many analytes including carotenoid,, However, equipment, reagents and
maintenance are otten too costly and too di fticult to arrange for many laboratories in
deveioping Countries Thus other methods such as ,pectrophotornetry often combined
with open colun chromatography (OCC) and are still commonly used in many
countries for analyzing the carotenoid content of foods
The data collected have been evaluated with respect to sampling procedures, number
of samples analyzed, analytical methou and method validation For this purpose, the
following classification system has been establ;shed:
H. HPLC data
R. OCC dat,
0. Other data
a/b/c data classified according to the CAREX system (a, b, and c ratings, see below)
k data for which adequate procedures appear to have been followed but detailed
information was not available for each step of the method
n data for which documentation i insufficient to allow pioper evaluation
z data of pooi quahiv
For example, HPLIC datm for which no documentation is available is given a 'Hn'
rating.
The CAREX expert system (97) was developed to evaluate data for the carotenoid
content of food in five general categorie,, number of ,amples, analytical method,
sample handling, sampling plan and analytical (lilality control. Data c, ,rideied
acceptable by this system represent the highest quality information on catotenoids.
CARX quality ratings assume that HPLC is used and depend on sampling
procedures, analytical method, the extent to which external scandards are used, the
eytent to which procedures ire documented, and emphasizes how representative the
carotenoid ,alues are for US foods Data acceptable to the CAREX sy',,tem are
classified as 'a', 'b' or 'c' but much of the data evaluated which gained a CAREX
rating were classified in 'c' which is the lowest class
Much of the data included in the data base were found to be not acceptable by the
criteria of the CAREX system because )f the lack of sufficient documentation.
However, such data can still be useful, especially when no other data are available.
Therefore, data which lack full details on sampling, analytical and validation
procedures were classified as 'k'
Data for which there was little or no description of sampling procedures or analytical
methods were classified in the 'n' category Data deived using methods regarded as
unsatisfactory aic classified as 'z' Such data include those derived using
spectrophotometry without prior separation by open-column d6romatography because
the values so obtain d are inflated by the incluion of carotenoids which are not
precursors of vitamin A
Intile data presented in Chapter 3, the principle method used is given together with
the data quality rating. Reference to the extraction and chiomatographic procedures
are also included. A summary of the methods used is provided in Chopter 4 while the
references to the data and to the niethods used are given in Chapter 7
5
Development of database files
It is essential that all information, which is received and found relevant, is entered
into a database structure This allows for easy search and retrieval operations and
The database management system dBASE IV version 15 was used to capture the
data The advantages ot this software package are its ,ide-spread use and availability,
established import and export features for data exchange with other software, and
ability to maniu)Lilate related data files A ti tLie relational databa-e management
system does have some advantages over dBASE IV in terms of the number of
variables and the ability of reltring variables to one anothei However, for the present
purpose, the features of dI3ASL, IV were regarded as sufficient
The data have been entered into three files differing in structure and content, and
referred to as tood.dbl, method.dbf and data.dbf (see Appendix 2 for structure of the
files)
* The file food.dbf contains inrornation on the names, origin and description of
food, The miil t-taceted description en,,ures that each food is Uniq,oly
identified
The file niethod.dbf describes the methods used tor the identification and
quantification of the carotenoids for each food It contains information on
procedures for extraction, saponification, chiomatography and analytical quality
control Modifications of established analytical procedures are flagged and
specified
Fhe file data.dbf contains the data for each carotenoid for all foods. For
practical reasons, it includes some of the information already present in the
food dbf file
The food dbf and data dbt files are linked by a number (foodnr) that uniquely
identifies each food in these files A second link is created by the reference number
(refnr) in the data dbf and method dbf files. Using thee two link fields, the
information from the three data tiles can be co)nbined to produce custom databases
The selection and presentation of the data in Chapter 3 is described in Appendix 3.
Initially, references to sources ot the daui were also entered in dBASE However,
since the program has only linited ability to produce various characters on both
screen and paper, the references (Chapter 7) were entered in WordPerfect format.
Introductory notes
Foods included in this table are listed according to the following Data are expressed as )g per 100g edib'e portion, and values in
food groups cereals, dai-y products, eggs, fats, fish, fruit, meat, other units were re-calculated to this method of presentation This
oils and vegetables. This gfouping is based on the Eurocode food resulted in a lower number of significant digits for these foods In
classification system The te-:,-rereal has been used for grain and most cases, the standard deviations or ranges were used as
grain products and the terni dairy products for milk and milk measure of variability.
products
Where the number of analyses (n) was aailable, standard errors
Within each food group, foods are listed alphabetically in English were transformed into standard deviations (SD = SE x Vn)
with their scientific names In most cases, the classification Values expressed as dry weight. for which no data on moisture
extends to genus and species and, where possible, foods are content were available, were excluded Some of the minimum and
classified to subspecies or variety maximum values reported in the table do not necessarily represent
the actual values originally reported but are minima and maxima
For some foods, the English name was not known and was of aggregated means.
replaced by either a transliteration or the local name.
- Retinol equivalents were calculated using the conversion factors
from the paper by Underwood (100) Data for total carotenoid
ii. Missing information values were not calculated from the individual carotenoids but
were listed as such in the publication
Missing entries in a particular column indicate that no nformation
was available in the original article. Thus such values should not - The data derived from reference 70 usually are usually comprised
be considered as zero. of mean values of data for foods from several Chinese provinces
or cities.
7
iv. Abbreviations and symbols
EP Edib!e portion of the food (if given) [a] B-c-yptoxanthin based on cryptoxanthin values
Mst Moisture content of the food (if given) [b] B-cryptoxanthin = free cryptoxanthin
a-carot a-Carotene [c] Estimated from figure
B-carot B-Carotene [d] Average of values for wild and cultivated varieties
3-crvpto B-Crypto\anthin [e] Average -f two cooking methods
Zeaxanth Zea\anthin [f] Boiled ir ,alted water
RE Retinol equivalents [g] Boiled in urisalted water
Princ method Principal anaxltical method [p] Impossible to calculate from data provided
HPLC High-performance liquid chromLtography
OCC Open column chromatography
Ext.ref. Reference to e\traction method
Chr ref. Reference to chromatographic method
Ref Reference number to the original
publication in Chapter 7
QM Quality measure
NA Not analyzed
ND Not determined
tr Trace values
7 (Malaee549
Ghee54
92 HPLC 11.1 06 ik
t)
'5 Icecream
(Croatia) 50 [p] OCC 85 85 Rn
()
7 Malted milk
powder
(Malaysia) HPLC 71 1 06 k
()
7 (Malai)
margarine89 897
150 HPLC 71 1 j6 Hk
()
62 Milk, huiian 100 3 5 12 4
human, first morning (USA) 1 KPIC 62 Hk
()
Sweet condensed milk
filled CMalaysia) 6 HPLC 71.1 06 Hk
C)
7 Sweetend condensed milk
(Malaysia) 53 HPLC 71 1 06 Hk
()
7 ULtra-heat-treated milk
(Malaysia) 20 HPLC 71.1 06 Hk
()
91 Whipping cream
(Netherlands) 200 33 HPLC 91 91 He
()
10
7 Egg 10 2 HPLC 71 1 06 Hk
salted (Malaysia)
)
7 Fgg 4 1 HPLC 71 1 06 Hk
century (Malaysia)
)
11
FATS
CFutenoid content of food (mqi1OOg ediote portic, f'esh wegh t)
85 Margarine
(Croatia) 230 [p] OCC 85 85 Rn
()
85 Margarine
'DeLcates'
(Croatia)
500 [p] OCC 85 85 Rn
()
91 Mayonnaise
(Netherlands) 42 7 HPLC 91 91 Hc
()
12
(Sepia officinalis)
13
14
15
16
17
Carotenoid content of food (ug/lOOg edible Portion. fresh weight)
18
19
20
21
Carotenoid content of food (ag/lOOg edible Portion. fresh weight)
84 Banana, raw
(CIS) 600 [p] unknown 84 84
(Musa paradisica)
91 Banana
20 3 HPLC 91 91 Hc
(Netherlands)
(Musa paradisica)
45 Banana (apple), raw fruit 17 27 6 7 KPLC
(Mexico) 45 45 Hk
(Musa acumilnata)
45 Banana (apple), raw fruit 14 34 30 10 HPLC
(Mexico) 45 45 Hk
(Musa acuminata)
70 Banana, dwarf, raw 57 79.8
'fragrant banana' (China) 117 0 0CC 70 70 Rn
17r-0 0 R
(Musa acuminata) W-250)
88 BiLberries, raw
865 344 [p] OCC
(Slovak republic) 88 88 Rz
(Vacciniun myrtillis)
88 Black currants, raw
36 446 [p] OLC
(Slovak republic) 88 88 Rz
(29-46) (425-474)
(Ribes nigrum)
22
23
Cdrotenoid content of food (g/100g edible portion. fresh weight)
24
25
26
27
28
84 Gooseoerry, raw
20 [p] unkncwn 84 84 -
(CIS)
()
15 Grapefruit 100 89.0 9 12 0
(U K ) 3 HPLC 77 77 Hk
(Citrus paradisi)
36 Grapfruit, raw fruit 360 30 [b] 63 HPLC 36 36 Hk
Texas (USA) (ripe)
(Citrus p3radisi)
36 Crioefrtjit, raw fruit 260 30 [b] 46 HPLC 36 36 Hk
Coachella (USA) (ripe)
(Citrus paradisi)
82 Grapefruit, fresh juice 0 [p] 0CC 71 71 Rn
(Egypt)
(Citrus paradisi)
82 Grapefruit, fresh fruit 200 [p] OCC 71 71 Rn
(Egypt)
(Citrus paradisi)
84 Grapefruit, raw
20 [p] unknown 84 84 -
(CIS)
(Citrus paradisi)
87 Grapefruit, canned 0 0 0 HPLC 87 87 Hn
(New Zealand)
(Citrus paradisi)
87 Grapefruit, raw 0 0 0 HPLC 87 87 Hn
(New Zealand)
(Citrus paradisi)
87 Grapefruit, raw flesh 41 88.4 0 0 0 HPLC 87 87 Hn
, Morrsion's (New Zealand)
(Citrus hybrid)
91 Grapefruit 0 0 HPLC 91 91 Hc
(Netherlands)
(Citrus paradisi)
29
Carotenoid content of food (g/lOOg edible portion, fresh weight)
30
31
79 Jumibo, raw
40 7 HPLC 79 Hn
(India)
(Syzyglum)
28 Kiwifruit, raw fruit 36 290 6 HPLC 28 78.1 Nc
Hayward (Spai) (ripe)
(Actinidia chinensis)
28 Kiwifrult, raw fruit 65 259 11 HPLC 28 78 1 Hc
Abbot (Spain) (ripe)
(Actinidia chinensis)
28 Kiwifruit, raw fruit 5 316 1 HPLC 28 78 1Hc
Monty (Spain) (ripe)
(Actinidia chinensis)
28 Kiwlfruit, raw fruit 41 281 7 HPLC 28 78.1 Hc
Bruno (Span) (ripe)
(Actinidia chinensis)
56 Kiwifruit, fresh pulp, seeds 80 82.3 20 70 0 13 0CC 72.1 72 1 Rk
Australian grown (Australia)
(Actinidia chinensis)
56 Kiwifruit, fresh pulp,0
New Zealand grown (Australia) 10 CC 72.1 72 1 Rk
pu 2R5
(Actinidia chinensis) OCsed2.5832
32
33
34
35
83 Mango
5000 Cc] unknown 83 83 0
(Mexico)
(Mangifera indica)
18 Mango Amelie, dried 17.0 < 60 2640 445 HPLC 74 - Hn
AmeLie (Mali)
(Mangifera indica)
18 Mango Batiste, dried 11.0 < 50 6080 1018 HPLC 74 Hn
Batiste (Haiti)
(Mangifera indica)
18 Mango Nimrode, dried 18.0 < 60 2830 477 HPLC 74 Hn
Nimrode (Mali)
(Mangifera indica)
61 Mango juice juice 530 88 HPLC 51 61 Hn
(Brazil)
(Mangifera indica)
6 Mango, black-gold fruit 0 615 0 0 0 103 HPLC 71.1 06 Hk
(Malaysia) (mature)
(Mangifera indica)
70 Mango, small, raw 45 80.4 850 0 OCC 70 70 Rn
(China)
(Mangifera silvatica)
15 4eLon flesh 100 92.1 16 990 0 166 HPLC 77 77 Hk
Cantaloupe (U.K)
(Cucuriis melo cantLoupens.)
45 Melon, raw fruit ND ND ND 0 HPLC 45 45 Hk
Casaba (USA)
(Cucumis meto)
45 Melon, raw fruit ND 47 ND 8 HPLC 45 45 Hk
Canary (USA)
(Cucumis meto)
36
37
84 Mulberry fruit
20 [p] unknown 84 84
(CIS)
(Morus alba)
96 Mulberry, raw 0 10 0 2 HPLC 96 96 Hn
(Australia)
(Morus alba)
70 Mulberry, purple, raw 95 82.0 10 0 OCC 70 70 Rn
(China)
(Morus alb)
70 Mulberry, white, raw 94 85.0 10 0 OCC 70 70 Rn
(China)
(Morus alba)
6 Musk lime fruit 0 12 446 65 0 43 HPLC 71.1 06 HK
(Malaysia) (mature)
(Citrus microcarpa)
60 Nectarine, fresh 93 87.1 3 25 97 [p] OCC 72.1 72.1 Rk
Golcknine (Australia)
(Prunus persica)
38
56 Olive, green, fresh puLp, skin 73 78.1 100 200 20 44 OCC 72.1 72 1 Rk
green skin and flesh (Australia)
(Olea europaea,
39
47 Orange
(Vietnam)
3500 [p] HPLC/OCC 71 71 Hn
(Citrus nobilis)
70 Orange, rav
63 88.3
(China) 50 OCt 70 70 Rn
(Citrus spp.)
82 Orange, fresh
juice
(Egypt) 2600 [p] OCt 71 71 Rn
(Citrus aurantijum)
79 Orange,
raw
(India) 190 32 HPLC 79 Hn
(Citrus aurantilzm)
40
41
42
43
Ref Variety
Food description Part of plant
(country) or animal EP Mst
(Scientific name) a-carot P-carot f-crypto Lutein Lycopene
(Maturity) () Zeaxanth Total RE Princ. Ext. Chr OM
(range) (range) (range) (range) (range) (range) (range) method ref ref.
45 Passion fruit, purple, raw fruit ND 336 42 60 HPLC 45 45 Hk
(USA)
(Passiflora eduLis)
45 Passion fruit, yellow, raw fruit ND 300 40 54 HPLC 45 45 Hk
(USA)
(Passiflora edulis)
45 Passion fiuit, yellow, raw fruit 70 750 53 13o HPLC 45 45 Hk
(USA)
(Passiftora edulis)
54 Passionfruit, raw pulp 470
Manao (Brazil. Sao Pau) 78 OCC -7 67.4 Rk
(Passiftora edutls)
56 Pawpaw (papaya), fresh
orange flesh (Austr.) (Australia) 70 89.3 0 240 1350
10LP
164 OCC 72.1 72 1Rk
(Carica papaya)
15 Peach, raw
flesh ana skin 100 88.4 tr 45 26
(U K ) 10 HPLC 77 77 Hk
(Prunus persica)
15 Peach, dried flesh 100 15.5 tr 345 200
(U.K ) 76 HPLC 77 77 Hk
(Prunus persica)
36 Peach, raw
fruit 60 30 [b1
Elberta (USA) (ripe) 13 HPLC 36 36 Hk
(Prunus persica)
36 Peach, raw
fruit 220 40 Cb4
Cling (USA) (ripe) 40 HPLC 36 36 Hk
(Prunjs persica)
27 Peach, raw fruit, peeled 69 86.5 4 64 74 16
(Spain) 31 18 HPLC 74 01.2 Hc
(ripe)
(Hybrid Prunus persi(a)
60 Peach, fresh
96 87.6 130
Blackburn (Australia) 1425 p] OCC 72 1 72.1Rk
(Prunus persica)
60 Peach, fresh
91 88.5 165
Elberta (Australia) 1500 IN OCC 72.1 72.1Rk
(Prunus persica)
44
45
Carotenoid content of food (g/100q edibe portion. fresh weight)
46
47
Carotenoid content of food (gg/lOOg edibte Portion, fresh weight)
48
49
70 Pineapple, raw
(China) 56 87.8
85 0 OCC 70 70 Rn
(Ananas spp )(80-90)
84 Pineapple, raw
(CIS) 40 [p] unknown 84 84 -
(Ananas spp.)
50
84 Plum, stewed
0 (p] unknown 84 84
(CIS)
(Prunub spp )
84 tijr, raw
100 ip] unknown 84 84
(C'S)
(P-unus spp )
51
52
85 Prune, dr.-d
1530 p] 0CC 85 85 Rn
(Croatia)
()
53
56 Rambutan, fresh
puLp 39 79 8 07'
red skin (Australia) OCC 72 1 72 1 Rk
(Ilephetlium Lappaceum)
56 Rarbutan, fresh
PULP 41 79.2 0
yellow skin (Australia) OC 72.1 72 1 Rk
Rk
(Nephetium tappaceum)
84 Raspberries, raw
(CIS) 200 [p] unknown 84 84
(Rubus idaeus)
84 Raspberries
jam
(CIS) 20 [p] unknown 84 84
(Rubus idaeus)
38 Raspberries, raw
73 251
(Slovak repuct c) [p] 0CC 88 88 Rz
(tr-17) (160-328)
(Rubus idaeus)-329)
87 Raspberries, raw
0
(New Zea and) 0 HPLC 87 87 Hn
(Rubus idaeus)
87 Raspberries, canned
0
(New Zealand) HPLC 87 87 Hn
(Rubus idaeus)
96 Raspberries, canned, drained
18 tr 0
(Australa) HPLC 96 96 Hn
(Rubus idaeus)
96 Raspberries, canned
7 tr 0
(Australi) HPLC 96 96 Hn
(Rubus idaeus)
88 Red currants, raw
(Slovak republic) 112 [p] OC a8 88 Rz
(Ribes rutrum) (57-160)
45 Red raspberry, raw
fruit 24 2 ND
(USA) HPLC 45 45 Hk
(Rubus spp )
54
33 Rose hip fruit (berries) 7210 1750 920 11120 1363 HPLC nf 33 Hn
Wild (Poland)
(Rosa canina)
33 Rose hip fruit (berries) 1760 1910 430 4330 469 HPLC nf 33 Hn
Cultivated (Poland)
(Rose rugosa)
55
56
56 Taarillo, fresh pulp, seeds 94 86.2 10 400 1050 164 OCC 72 1 72.1 Rk
New Zealand grown (Australia)
tCyA,omandra betacea)
57
84 Tangerine, raw
(CIS) 60 Ip unknown 84 84
(Citrus reticulata)
84 Tangerine
juice/jams
(CIS) 30 [p] unknown 84 84
(Citrus reticuLata)
36 Tangerine concentrate
180 230 [b]
(USA, 51 HPLC 36 36 Hk
(Citrus reticulata)
36 Tangor concentrate
490 310
Murcott (USA) 110
1b] HPLC 36 36 Hk
()
6 Tree tomato fruit 0 599 1236 0
(Malaysia) 214 KPLC 71 1 06 Hk
(mature)
(Cypho'nandra betacea)
13 Tree tomato, raw pulp 790 1390 170 60 260 OCC 67 67 Rk
(Brazil) (ripe) (490-1180) (980-1820) (100-250) (20-110)
(Cyphomandra betacea)
13 Tree tomato, raw peel 880 1000 150 110
(Brazil) (ripe) 239 OCC 67 67 Rk
(620-1280) (680-1230) (110-170) (60-170)
(Cyphomandra betacea)
;0 Wamp,.e, raw
45 82.6
f C.,
i na) OCC 70 70 Rn
(CLausena lansium)
58
59
60
61
85 CF.,rot, oil
1130000 [p] aCC 85 85 Rn
extract (Croatia)
()
62
23 Palm oil, fresh fruit oiL 16370 36260 tr 730 tr 7468 oCC 23 14 1 Rk
Jacq tenera (Brazil. Una, Ba)
(Elaeis guineensis)
23 Palm oil, sterilized ster fruit oil 9410 22890 tr 930 tr 4634 OCC 23 14 1 Rk
Jacq tenera (Brazil, Una, Ba)
(Elaeis guineensis)
23 Palm oil, fresh fruit oil 42480 102610 3100 tr tr 21083 OCC 23 14 1 Rk
(Brazil, Una, Ba)
(ELaeis oleifera)
23 Palm oil, fresh fruit ail 1840 20210 tr 70 600 3528 OCC 23 14 1 Rk
Psifera (Brazil, Una, Ba)
(Elaeis guineensis)
23 Palm nil, 'resh fruit oil 29560 57610 tr 1720 tr 12173 oCC 23 14 1 Rk
Dura dumrpy (Brazil, Una, Ba)
(Elaeis guineensis)
25 Red palm oil, raw fruit, oil extr 13000 30000 6131 OCC 25 36 Rz
(Brazil, Recife.)
(Elaeis guineensis)
63
64
65
Carotenoid content of food (ug/lOOg edible portion, fresh weight)
66
67
Carotenoid content of food (eg/lOOg edible Portion. fresh weight)
68
Carotenoid content of food (eQ/lOOq edible portion. fresh weight)
69
70
71
Carotenoid content of food (g/lO09 edible portion, fresh weiLht)
9 Bitter gourd
moon shine (Taiwan) (maU - 90 [p] OCC 71 71 Rk
(MomordIca charantia)
9 Bitter gourd
KY No. 6 (Taiwan) (mature) 126 [p] OCC 71 71 Rk
(Momordica harantia)
9 Bitter gourd
KI No 2 (Taiwan) (mature) 138 [p] OCC 71 71 Rk
(Momordica charantia)
31 Bitter gourd, cooked
(Pakistan) 2210 368 OCc 71.4 71 1Rz
(Momordica charantia)
31 Bitter
gourd, raw
(Pakistan) 2840 473 OCC 71.4 71.1Rz
(Momordica charantia)
80Bttrgor
80 Bitter gourd 67
11 HPLC 80 80 Hn
(Nepal)671
(Momordica charatia) PC 8 80 H
72
73
74
75
Carotenoid content of food (ag/lOq edibte portion, fresh weight)
76
77
78
79
80
81
82
83
84
85
82 Chard, fresh
(Egypt) 10000 [p] 0Cc 71 71 Rn
100 [] OC 7 1 R
(Beta vulgaris)
2 Chard, Swiss
1760 293 HPLC 78 78 2 Mn
(Guatemala)
(Beta vuLgari1 ricta)
70 Chard, Swiss, raw 100 94.2
(China) 935 0 0CC 70 70 Rn
(570-78)
(Beta vulgaris)
70 Chard, Swiss, pate green, raw 90
820 0 0CC 70 70 Rn
(China)
(Beta vutgaris)
70 Chard, Swiss, purple, raw 95 95.0 1010 0 OCC 70 70 Rn
(China)
(Beta vulgaris)
70 Chard, Swiss, white, raw 95 94.4 1880 0 OCC 70 70 Rn
(China)
(Beta vutgaris)
86
87
88
89
90
40 Chinese chives, raw Leaves 97 15 3260 150 [a] 558 OCC 72 1 72.1 Rk
(Australia) (mature)
(All um tuberosum)
40 Chinese flowering cabbag, raw leaf, stem 94 15 1360 20 230 OCC 72 1 72 IRk
(Australia) (mature)
(Brassica parachinensls)
91
92
93
(Colacasma antlquosum)
52 C---,msa, green, raw 9081 1514 HPLC 52 52 Hk
(Co a:asia ant ic usurn)
31 Co'bash, cookEd 1050 175 OCC 71 4 71 1Rz
(Pakistan)
(Legenaria vutgaris)
31 Colbosh, aw 1580 263 OCC 71.4 71 1 RZ
(Pa, 1star)
(Legenaria vulgaris)
31 Colocaia, raw 1360 227 OCC 71.4 71 1 Rz
(Paiistar)
(Co'oca; a -sculenta)
31 Colocasia, cooked 910 152 OCC 71.4 71.1 Rz
(P,, stan)
(Colocasma escutenta)
94
47 Coriande
(Vietnam) 3?90 [p] HPLCOCC 71 71 Hn
(Coriandrum sativum)
95
96
97
98
47 Dismanthus
(Vietnam) 3550 (p] HPLC/OCC 71 71 Hn
(Neptunia oleracea)
79 Drumstick, raw 19690 3282 MPLC 7 Mn
(I nd i a)
(Moringa oleif-ra)
6 Drumstick leaves 0 7536 0 7128 0 1256 HPLC 71.1 06 Hk
(MaLa -ia)
'Moringa oleifera)
8 Drumstick Leaves leaves 0 7540 7130 1257 HPLC 71.3 06 Hk
(MaLaysa) (mature)
(Morir-ja oleifera)
79 Duggal Kura, raw 710 118 HPLC 79 Mn
(Lrda)
(Amaranthus uiridus,
79 Dusudutega, raw 530 88 MPLC 79 Mn
(India)
(Cocculus hirsutus)
Egg plant, big 89 90.2 35 6 HPLC 73 73 Hc
(Thailand)
(Solaniu xanthocarpn)
3 Egg plant, small 89 82.8 103 17 HPLC 73 73 HC
(Thailand)
(Solanum to-vum sw.)
9 Eggplant
(Tzwan) (mature) 36 [p] OCC 71 71 Rk
(SoLanum melongena)
99
Carotenoid content of fooa (g/lOOg edible portion. fresh weight)
100
101
102
103
Goa bean
(Thaiar) young pod 92 91.7 1139 190 PLC 73 73 Mc
(Psopthocarpus tetragon.)
86 Goa beans, fresh
36 6 HPLC 86
(Indonesia) 86 Mn
(Psophocarpus tetragonotob)
86 Goa beans, dried leaves 238 40 HPLC 86 86 Mn
(Indonesia)
(Psophocarpus tetragonolob)
86 Goa beans, boiled Leaves 16 3 HPLC 86 86 Mn
(Indonesia)
(Psophocarpus tetragonolob)
104
77 Green beans, boiled (g] pods and beans 100 91.0 39 310 0 55 HPLC 77 67 Hk
(U C )
(Phaseolus vuLgaris)
105
THoi
plum
(Thailand) leaves 48 78.5 1231
205 HPLC 73 73 Hc
(Spodias spp.)
3 Hoo-saew
Leaves 75 94.5
(Thailand) 1143
191 HPLC 73 73 Hc
C)
3 Horse radish
leaves 22 84.-
(Thailan) 5452 909 HPLC 73 73 c
(Moringa oLeifera)
3 Horse radish
pod 51 88.0
(Thailand) 114 19 HPLC 73 73 HC
(Moringa pterygosperma)
83 Huazontte
(Mexico) 770 [p] unknown 83 83 0
(Chenopodium nuta[liae)
106
107
108
109
110
lii
Carotenoid content of food (uQIOOQ edibte portion, fresh weight)
112
113
114
115
Carotenoid content of food (ag/iOOg edible portion. fresh weight)
9 Madeira vine
(Taiwan ) (mature) 1560 [p] OCC 71 71 Rk
()
116
117
Carotenoid content of food (ag/1OO edible Portion, fresh weight)
118
119
120
121
9 ML tard
(Taiwan) (mature) 1656 [pi
(Brassica jun~ea) OCC 71 71 Rk
9 Mustard
Shoi-ban (Taiwan) (mature) 780 [p] OCC 7. 71 Rk
(Brassica juncea)
122
123
124
125
Carotenoid content of food (pg/lOOg edible portion. fresh weight)
9 P3-Tsa,
2430 [p] OCC 71 71 Rk
Ji-Tan-Gun (Taiwan) (mature)
(Brassica campestris)
9 Pai-Tsai
1080 [p] OCC 71 71 Rk
(Taiwan) (mature)
(Brassica campestris)
9 Pal-Tsai
1188 [p] OCC 71 71 Rk
golden (Taiwan) (mature)
(Brassica cairipestrs)
9 Pai-Tsal
696 [p] OCC 71 71 Rk
Native (Taiwan) (mature)
(Brassica campestris)
3 Pak vra-done flower 57 81.0 403 67 HDLC 73 73 Hc
(Thailand)
(Coreya arborea)
3 Pak Kra-done 54 73.5 971 162 HPLC 73 73 Hc
(Thailand)
(Careya arborea)
3 Pak chee taos 49 88.1 1713 286 HPLC 73 73 Hc
(Thailand)
(Anethum graveolens)
3 Pak e-hin 78 93.7 1653 276 HPLC 73 73 Hc
(Thailand)
()
126
127
128
3 Pak wan
60 92.0 592
(Tha Land) 99 HPLC 73 73 Hc
(MarsiLea crenata)
3 Pak whan (ban) 43 82.5 3097 516 HPLC 73 73 Nc
(Thailand)
(Sauropus albican)
43 Pabang sak, cooked [el
4750
leafy type (Bangladesh) 792 OCC 43 43 Rk
(Spinacea oLeracea)
43 Palang sak, riw 6400
Leafy type (Bangladesh) 1067 0CC 43 43 Rk
(5040-7690)
(Spinacea oLeracea)
3 Pang-pui
48 87.9 366
(ThaiLand) 61 HPLC 73 73 HC
()
79 Panpandlak-a, raw 1280 213 NPLC 79 Hn
(India)
()
129
130
131
132
133
134
29 Pepper, red, raw fruit 57 5 43000 14000 3000 34000 8455 HPLC 29 29 tn
Americano (Spain) (mature)
(Capsicun annuum)
29 Pepper, red, raw fruit 85.9 156000 51000 9000 53000 30692 HPLC 29 29 Hn
ArrLerBSEB1 (Spain) (mature)
(Capsicum annuum)
29 Pepper, red, raw fruit 39.3 105000 30000 35000 26000 20260 HPLC 29 29 Hn
NAN (Spain) (mature)
(Caps cun annuum)
29 Pepper, red, raw fruit 22.1 35000 16000 7000 27000 7305 HPLC 29 29 Hn
Atbar x M CA (Spain) (mature)
(Capsicum annuum)
29 Pepper, red, raw fruit 85.7 113000 41000 13000 58000 22605 HPLC 29 29 Hn
AmLerB51 (Spain) (mature)
(Capsicum annuum)
29 Pepper, red, raw fruit 82.5 122000 35000 60000 23553 hPLC 29 29 Hn
Betrubi (Spain) (mature)
(Capsicum annuum)
29 Pepper, red, raw fruit 58.1 109000 42000 17000 43000 22031 HPLC 29 29 Hn
Negra (Spa) (mature)
(Capsicum annuum)
135
136
137
Carotenoid cont-nt of food (eg/lOOg edible Dortion. fresh weight)
138
139
140
141
2 Radish
4500
(Guatemala) 750 HPLC 78 78 2 Hn
(Raphanus sativus)
9 Radish
(Raish
60 [p] OCC 71 71 Rk
(Taiwan) (mature)
(Raphanus sativus)
9 Radish
9Radis
Ming-ho (Taiwan) (mature)
12 [pN 0CC 71 71 Rk
(Raphanus sativus)
142
143
Carotenoid content of food (eg/lOOg edible Portion. fresh weight)
144
145
146
147
Carotenoid content of food (g/100g edible portion. fresh weight)
148
149
150
151
152
153
154
155
3 Sugar pea
(Thaliand) young pod 88 88.4 12 HPLC 73 73 HC
70
(Pisum sativum)
3 Swamp cabbage white stem 81 94.0 1222 204 IPLC 73 73 Hc
(Thailand)
(Ipomoea reptans)
156
157
158
159
160
161
162
163
164
165
166
167
168
169
9 Wax gourd
(Taiwan) (mature)
66 [p] OCC 71 '1 Rk
(Benincasa hispida)
40 Wax gourd, raw
fruit (Large) 68 20
(Australia) 10
(mature) 3 OCC 72.1 72.1RK
tBenincasa hispida)
70 J3ax-gourd, raw
78 97.0
(Ch'na) OCC 70 70 Rn
(Benincasa hispida)
9 Welsh onion
(Tawan) (mature) 1938 [p] OCC
(Altium fistulosum) 71 71 Rk
91 White cabbage,
cooked
(Netherlands) HPLC 91 91 Mc
(6 oLeracea capitata)
3 Jild ginger
75 93.7 0
(Thailand) HPLC 73 73 Hc
(KaeMPTeria pandurata)
9 Winged bean
(Taiwan) root
(mature) [p] OCC 71 71 Rk
(Psophocarpus tetragon.)
9 Winged bean
(Taiwan) (mature) 378 [p] OCC 71 71 Rk
(Psophocarpus tetrago )
9 Winged bean
(Taiwan) leaves
(mature) 4860 [p] OCC 71 71 Rk
(Psophocarpus tetragon.)
80 Winged bear leaves
Leaves
(Nepal) 13135 2189 HPLC 80 80 Hn
(Psehocarpus tetragon.)
6 Wotrberry Leaves
0 5867 0 7591
(Malaysia) 0 978 HPLC 71.1 06 Hk
(Lyciurm chinense)
170
171
172
absorb. Absorbence
ACN Acetorutrile
CH2C12 Dichloroinethane
co-chr. Co-chromatography
CR Chemical reactions
BHT Butylated hydroxytoluene
Fluor. Fluorometry
HPLC High-performance liquid chromatography
MeOH Methanol
MS Mass spectrometry
NMR Nuciear magnetic resonance spectroscopy
OCC Open column chromatography
ODS Octadecylsilane
PE Petroleum ether
QM Quality measure
RT Retention time
Spectr Spectrophotometry
THF Tetrahydroturan
TLC Thin layer chromatography
Note: The numbers in the ext ref and chrom.ref columns refer to the methods of extraction and chromatography respectively, as described
in the original publications listed in Chapter 7. Whcn a method used is based on an earlier published method this is indicated by giving
the reference number of the initial method followed by a decimal point and a serial digit. For example, 67.4 in the column for
chromatography indicates the fourth modification listed of the method described by Rodriguez et al. in reference 67.
173
Reference Reference Methd Colurri ELuent omposition/fto. Peak identification/ Internal standard GM
Ext Sap Chr Name/size Diam Type quantification
01 Granado et at. (1992) 74 2 n 01 HPLC Spher,-5 ACN/CH2CL2/MoOH (70 20 10), ACN/MeOH (85 15) RT/stop ftcw scan, co-chr. Echinenone, retinyl paLmitate Hb
220x4 6 5 I i.ocr/grad 1 8 3 5 nt/mi absorbance
02 Lopez-Palacios et at (1993) 78 78 2 HPLC Hn
174
Reference Reference Method Colurvi Etuent composition/fLow Peak identification/ Internal standard ON
Ext Sap Chr Name/size Dam Type quantification
18 Bushway (unpublished) 74 n - HPLC
Hn
175
Reference Reference Method Coturnn ELuent composition/flow Peak identification/ Internal standard ON
Ext Sap Chr Ndme/size Diam Type quantification
35. Mlnguez-Mosquera et aL. (1992) 39 1 1 35 HPLC Spherisorb H20/reagent/MeOH (1.1,8) i acetone/MeOH (1 1) RT, absorb.
Hk
250x4 5 gradient 2 mr/min peak area
36 Philip e, at. (103G^) 36 n 36 HPLC Waters resolve MeOH-ethylacetate absorb Sudan 1 Hk
1503 9 gradient 1 mL/min intern std
37 Chen et at. (19921 31 n 37 HPLC Phenonenex ACN/MeO,/CHCt3/hexane (75 12 5 7 5 7 5) RT, TLC, co-chr. P-apo 8 carotenal Hb
250x4 6 5 ji isocratic 1 mI/non intern std.
38. Christophersen et at. (1989) 38 n 38 HPLC SupeLco MeOH-H20/ethyt acetate (80.20) RT, TLC Hk
250x4.6 5 g isocratic peak area
39 Minguez-Mosquera et at (1990) 39 1 39 TLC GF 254 PE/acetone/diethyLamine (10:4:1) absorb., color in UV Rz
absorbance
40 Wilts et at. (1984) 72.1 n 72.1 0CC acetone/PE (1-4%) absorb Rk
absorbance
41 Genhura et at. (unpublished) 41 n 41 OCC acetone/PE (4%)
Rk
absorbance
42. Khachik et al (1992) 42 n 42 HP.C hicrosorb ACN/MeOH/CH2CL2/hexane RT, MS, absorb., CR P-apo 8 carotenat/ate, nonapre Hb
250x4 6 5 A combined varying intern. std.
43 Rahman et at. (1990) 43 n 43 OCC Brockman PE absorb Rk
44 Oaood et at. (1989) 44 y 44 HPLC ChromasiL ACN/MeOh/ethy acetate (53 40-7) TLC, absorb. Hk
250x4.6 10 g isocratic varying not described
45. Homnava et at. (1990) 45 y 45 HPLC Zorbax ACN/methylene-Ct/tri-ethytamine (350:150 1) RT Hk
250x4 6 5 IL isocratic I mI/mn peak area
46 Sian et at (1991) 71.2 n 46 Spectr.
0
ebsorbance
47. Huynh Hong et at (unpublished 71 ? 71 HPLC/OCC
Hn
176
Reference Reference Method Coturr Etuent cornpositionlftow Peak identification/ Internal standard OM
Ext Sap Chr Name/size Diam Type quantification
52 Dikshit et aL. (1988) 52 y 52 HPLC Micropak ACN/MeOH (80 20) RT Nk
300x4 10 A. :ratic 1 2 m/mn peak area
53 Gregory et at (1987) 53 y 53 HPLC Waters resolve MeOH/ethyL acetate (linear) RT, TLC, absorb CR cantaxanthin, capsanthin Ic
150x3 9 gradient 1 8 mi/mn intern. std.
54 Godoy et at. (submitted) 67 n 67 4 0CC PE/ethyLether(5%)-PE/acetone(1C%)-pE TLC, absorb., CR Rk
absorbanz:.
55 Wilts et at. (1986A) 72.1 n 72 1 0CC acetone/PE (1-4%) absorb. Rk
absorbance
)6 Wilts et at (1986B) 72 1 n 72 1 0CC acetone/PE (1-4%) absorb Rk
absorbance
57 Wilts et at. (1986C) 72 1 n 72.1 0CC acetone/PE (1-4%) absorb Rk
absorbance
58. Wilts et at (1987A) 72.1 n 72 1 0CC acetone/PE (1-4%) absorb Rk
absorbance
59. Wiltt et at (19878) 72 1 n 72.1 0CC acetone/PE (1-4%) absorb. Rk
absorbance
60. Wilts et al (1983) 72.1 n 72 1 0CC acetone/PE (1-4%) absorb Rk
absorbance
61 Wilberg et at. (paper, 1993) 61 61 HPLC Waters RT, spiking, TLC, absorb., CR Hn
peak area
62 Kim et at (1990) 62 y 62 HPLC Zorbax ACN CH2CL2.MeOH (7:2:1) absorb Hk
250x4 6 7 IL isocratic peak area
63. Mejia et at. (1988) 74 3 n 75 HPLC Novapak acetone/MeOH/THF (58:38:7) RT/literature Hk
150x3 9 4 a isocratic 1 mt/mn peak height
64 Mercadante et at. (1991) 67 n 67 8 0CC acetone in PE TLC, absorb , CR Rk
absorbance
65. Philip et at (1988B) 36 n 36 HPLC Waters resolve MeOH-ethylacetate absorb. Sudan 1 Hk
150x3 9 gradient 1 mL/mn intern. std.
66. Moorthy et at. (1990) 71 n 71 OCC acetone/hexane absorb Rk
Isocratic absorbance
67. Rodriguez et at (1976) 67 y 67 OCC acetone/e-hyL ether TLC, abso-b., co-chr. Rk
absorbance
68. Rouseff (1992) 68 n 68 HPLC Analytichem ACN/CH2CL2/MeOH (65.25.10) RT, absorb., literature Hk
250x4 6 isocratic 1 mI/mn peak area
177
Reference Reference Method CotLfrn ELuent composition/fLow Peak identification/ Internal standard GM
Ext Sap Chr Name/size Diam Type quantification
69 ldvares et at (submiitted) 67 n 67.9 0CC acetone-PE I H20-acetone I ether-PE TLC, absorb., CR Rk
absorbance
70. Ershuw et at. (1990) 70 7 70 OCC
Rn
178
SUMMARY OF METHQOS USED FrR THE ANALYSIS OF CAROTENOIDS
Reference Reference Method Coturi Etuent composition/ftow Peak identification/ Internal standard OM
Ext Sao Chr Name/size Diam Type quantification
86 VITAL (1993) 86 86 HPLC
in
179
181
English name Local name English name Local name
Malanga Osh Malaysia
Mint Ye rb a b u e n a Plum. red Buah plum
Nightshade Macuy Banana Pisang tanduk
Parsley Perej ii Carrot Lotak me ah
Purslane Verdolaga Cashew le.ves Pucuk
Radish Hoja de rabano Ceylon spinach Remayong
Spinach Espinaca Chili pepper, green Lada hijau
Sweet potato Camote Chili, red Lada merah
Turnip Hojas de nabo Chine,,e cab'Jage Pak-coy
Watercress tBerro Chinese chives , cai
Wax beans Hojas de frijol Chinee kale Kai-lan-co
Chinese mustard leaves Sawi
Indonesia Coriander leaves Daun ketumbar
Amaranth Bayam Curry leaves Karrupillay
Cassava Singkong, daun Daun kelor Cekur mais
Chinese cabbage Caisin Drumstick lea-es Daun ke'
French beans Buncis Fern shoots Pucuk paku
Goa beans Kecipir Four-angled bean Kacang botor
Indian mulberry Mengkudu, daui" French bean Kacang buncis
Maize Jagung Jackfruit Nangka
Papaya Pepaya, daun Lettuce Sang-coy
Sauropus Katuk, daun Long bean Kacang panjang
Swamp cabbage Kangkung Mandarin orange imau Cina
Swkeet potato, orange Ubi jalar merah Mango, black-gold Miangga
Sweet potato, white Ubi jalqr putih Mint leaves Daun pudina
Taro Talas, daun Musk lime Lirnau kesturi
Yardlong bean Kacang panjang Orange Limau manis
Papaya Betik
182
English name Local name English name Local name
Papaya shoots Daun beti Chilli, red Rato Khursani
Paprika, Bell pepper Lada hijau besar Cho-cho marrow Squash
Pegaga gajah Pegaga gajah Curli doek Halhale saag
Pumpkin Labu merah Fern shoots Neuro
Salted vegetables Hum-coy Garden cress Chamsur
Sesbama Daun turl Indian spinach Poi saag kavro
Snake gourd Ketola ular Ladies fingei Ramtoriya
Spinach Bayan putih Lamb's quarter Bethe saag
Spring onion Daun bawang Lettuce Jln saag
Starfruit Belimbing manis Mint Pudina
S\,amp cabbage Kangkung Mustard leaves Tori saag
Tapioca snoots Pucuk ubi kayu Pigwid Lunde
Terung pipit Terung pipit PigVlg Ban lunde
Tree tomato Tomato opkok Prostrate Bhangi raj
Watermelon, red Tembikai Pumpkin Pakeko Fursi
Wolfberry leaves Kau-kei-coy Pumpkin leaves Phars, munta
Yam stalks Ba~ang keladi Pumpkin, green Hariyo Fursi
Rape leaves Rayo saag
Nepal Rape shoots, tender Duku saag
Amaranth Latte saag Spinach Palung
Asparagus shoots Kurilo Stinging nettle Sisnoo
Bitter gourd Tite Karela Sweet potato Sakharkand
Brinjal Bhanta/Bhindi Tomato Tamatar
Buckweat leaves Fafar ko saag Turnip Salgum
Cabbage Bandakobi Water cress Sim rayo
Carrot Gazer Yarba-de-tega Piro ghanse
Cauliflower Cauli
Chilli, green Hariyo Khursam
183
184
The best way to do this would be to establish a network of regional centres for analyzing
foods with particalar attention being paid to the analysis of carotenoids. From the
following list, a start could be made with two centres in Africa and one in each of the
other regions.
Africa
* Ethiopia
* Anglophone West Africa; for example, Nigeria or Ghana
* Eastern and Southern Africa; for example, Tanzania or Zimbabwe
* Francophone West Africa; for example, Senegal
* North Africa, for example, Egypt
Asia
* Indonesia
* India
* Th,; Phiippines
Latin America
* Brazil
* Mexico
* Guatemala
Such centres could act as training and reference centres for nearby countries. They wo:ld
need to be supported by established centres in industrialized countries which could assist
with training, quality control, and the development and testing of appropriate methods.
An appropriate organizational structure will need to be established and the necessary
funds obtained.
185
Data quality and methods used
Data originating from developing countrie.s were generally not determined by HPLC and
this explains why much of these data were not up to CAREX standards Furthermore,
the CAREX confidence codes are also determined by the number of acceptable values
for a given food As most of the foods in the database are not aggregated to any extent,
it is to be expected that the data quality will be low according to these standards.
In addition, the CAREX system does require that there is sufficient documentation to
allow data to be evaluated properly. Thus estimates given in this report of data quality
for many foods from developing countries are possibly lower than that appropriate to the
data. Often, reference to the sorce of data was missing oi impossible to locate Most
of the data of high quality conics froin fev, laboratories
The proportions of data of various quality are presented in Table 6 1. Although the best
method for determining the individual carotenoid content of foods is hy using HPLC,
open-column chromatography can give accurate estimates of the provitamin A content
of foods, provided that other aspects of the method are adequate and that the procedures
are well documented Good agreement between HPLC and OCC values as, for example
in Carvalho et al. (20), attest to the reliability of this method. However, there is a need
to establish criteria for better evaluating data obtained by open-column chromatography.
The data found in national food composition tables are generally hjg':er than those
presented in Chapter 3 For example as shown !n Table 6 2, th data on the piovitanmin
A content of foods consumed in a recent study of pregnant women in Indonesia (D.
Suharno, C.E West, Muhilal D. Karyadi, J.G A J. Hautvast, in preparation) are high
compared with what could be regarded as the most appropriate estimates. Thus levision
of the data on the (pro)vitamin A content of foods has resulted in at least a 50%
reduction in the estimated intake of vitarun A.
186
Table 6.2 Comparison of provitamin A content of foods as listed in
Indonesian food composition tables and current table
Fruits
Avocado Apokat Persea 34a b 7a
americana
Vegetables
Carrot Wortel Daucus 180or ' 1000 d
carota
187
Table 6.2 (continued)
188
Table 6.2 (continued)
tables data
13
oleracea
5 0d 249
bean panjang unguiculata
Many of the problems encountered would oave been overcome if appropriate guidelines
for the production of data of adequate quality had been followed and adequately
described Achieving adequate data quality is not an unattainable goal but of obtaining
data of a quality sufficient for the purpose to vhich data will be put Fortunately a book
has recently been published which could readily act as a handbook for tho,,e generating
and reporting data on food composition The book (98), written by Dr leather
Greenfield and Professor David Southgate, should be consuilted by all people involved
in such work as it addre,,se,, the issue involved It will be u',ed as the basis for the
International Postgraduate Courses on the Production and U,,e of Food Composition
Data in Nutrition, tie second of which will be held in Wageningen, 3-21 October 1994.
People involved in generating and compiling data on the carotenoid content of foods
analysis would benefit from attending such a course
In addition, there is a need for more training on the specific aspects of carotenoid
analysis An initial training could be provided in courses. which could be held in an
industrialized country, but such training would need t-) be followed up by on-site training
and back-up over a period of years Such training would assume that manpower, facilities
and funds would be available for the analyticai work to be carried out
The specific problems encountered when examining the data included the following.
Identification ot f(od, Sometimes, the scientific name was used to identify the
appropriate English common name When no English name could be found, the
literal local name was transf)rmed into English The extent of descriptive
189
information provided in the original publications varied greatly among the food
items.
Sampling In general, procedures used for sampling of foods were described
inadequately Information on the origin and number of samples is vital for
assessing the quality of data
Time between sampling and analysis Generally, this was not reported in
publications Ideally, foods should be analyzed directly after sampling, preferably
on the same day. If this is not possible, fod samples should be stored
appropriately
Saponitication steps- Usually, the procedures followed were adequately described.
However more data or the carotenoid content of foods obtained using methods
with and without saponification are required.
Exposure to light and air- Descriptions of preliminary sample treatment often do
not indicate whether steps were taken to reduce such exposure. These are
necessary in order to minimize oxidation and isomerization of the carotenoids.
Analytical methods use( Fhe principle method used in Latin-America and some
of the Asian countries was open-column chromatography (OCC). In general,
investigators using OCC
* usually provided information on the concentration of the standard
solutions but only limited detail on the purity of the standard materials
used(, and
* usually restrict information on the identification of the carotenoids to
retention time and absorption
Thus investigators using OCC should provide sufficient detail to enable the
quality of the data produced to be evaluated.
Quality control procedure,, for HPLC. External standards, reference materials and
validation methods have often not been used by investigators carrying out analyses
by IHPLC The general reluctance to use internal standards, even though some are
now commercially available, can be seen from the data presented in Chapter 4
The use of internal standards such as B-apo-8-carotenal, ethyl 13-apo-8'
carotenoate and nonapreno-B-carotene is essential when using HPLC because of
possible losses e,,pecially (luring the extraction procedure.
190
1. Use of guidelines Those involved in generating and compiling data should follow
the guidelines set down by Greenfield and Southgate (98) and aim, whenever
possible, to produce data which we have classed a, Hla or Oa A description of
the method used in generating and selecting the data shoild always be given
Special attention should also be given to sampling procedure , the use of standard
reference materials of established purity, description of methods used to identify
individual carotenoids, and recoveries.
191
7. Standard reference materials: There is a need for the ready supply of standard
reference materials for carotenoids in foods. Both the United States National
Institute of Science and Technology (NIST) and the Community Bureau of
Reference of the Commission of the European Union (BCR) are active in the
area of standard reference materials but none are yet available for carotenod.,.
Therefore standard reference materials should be established, maintained and
made available at an affordable price to recognized institutions In addition,
training should be provided in the use of standard reference materials and of in
house materials with values linked to those of certified standard reference
materials.
192
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(fruus and vegetables) Geelong, Deakin University, 1993
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199
Philippines)
201
* Dr Vinodini Reddy, National lnttntute of Nutrition, 1lyderahad (India)
* Mrs Delia Rodriguez Amaya, Universidade de Campina,, Sao Paulo (Brazil)
* Dr iH Scherz, German Research Institute for Food Chemistry, Garching
(Germany)
* Protessor 1, Skihsted, KVI. Centre for Food Research, Frederiksherg (I)enmark)
* Dr Noel W Solomon,,, CeSSIAM, GuIteIala City, (Guatemala)
Dr FS Solon, Nutrition Center of the Philippines, Manilda (The Philippines)
Dr D A T Southgate, AFRC Food Research Institute, Norwich (U K )
Dr P Sungpuag, INMU, Salaya (Thailand)
(Hungary)
Professor C E West, Wageningen Agricultural University, Wageningen (the
Nethlerlands) and Emory University School of Public I iealth, Atlanta GA (U SA)
Dr Zewdie Wolde-Gebriel, Ethiopian Nutrition Institute, Addis beba (Ethiopia)
202
DATA.DBF
FIELD NAME FIELD I)ESCRIPTION
REFNR Reference number of the publication
FOODNR
Assigned food number
NAME ENGLI English name of the food
NAMESCIEN Scientific name of the food
COUNTRY
Country of origin of the food
BETAMN B-carotene values (mean)
BETA SD B-carotene values (standard deviation)
BETARG B-carotene values (range)
ALPltA_MN a-carotene viues (mean)
ALPHIASD a-carotene values (standaid deviation)
ALPItARG a-carotene values (range)
DELTA MN 8-carotene values (mean)
DELTASD 8-carotene values (standard deviation)
DELTARG 8-carotene valu~es (range)
GAMMA MN y- carotene values (mean)
GAMMASD y-carotene values (standard deviation)
GAMMA RG y-carotene values (range)
ZETA MN (-carotepe values (mean)
ZETA SD C-carotene values (standard deviation)
ZETA_RG -carotene ',alues (range)
ACRYPMN a-crypthoxanthin values (mean)
ACRYPSD a-crypthoxanthin values (,,tandard deviation)
A (RYP RG a-crypthoxanthin values (range)
B CRYP MN B-crypthoxanthin values (mean)
B_CRYPSD B-crypthoxanthin values (standard deviation)
B_CRYP RG _ B-crypthoxanthin values (range)
LYCO MN Lycopene values (mean)
203
APPENDIX 2. (continued)
FOOI).I)BF
FIELD NAME DESCRIPTION of FIEL)
REFNR Reference numher of the publication
FOODNR
Assgned toed nuiber
DATE ENFRY Date of entry
DATECHANG Date of entry chnges
NAMEENGLI English name of the food
NAME SCiEN Scientific name of the food
TYPE Type, stan, brand, variety
MATURIPF MNaturity/ripeness of plant, vegetable, fruit
PART PLANT Part of the plant, vegetable, fruit analysed
EDIBLEPOR Edible portion of the food (%)
EUROCODE 2 Eurocodce 2 tood number
N_SAMPLES Number of samples analysed
204
APPENDIX 2. (continued)
MOISTURE
Moisture content ot the food (%)
NOTES
Notes and other ienark,
METHOD.DBF
FIELD NAME FIELI) DESCRIPTION
REFNR Assigned reference nunmber
AUTHOR First author name of the reference
EXTR NR E ,traction method nunbcr
ANTISTAB Kind of anti-o\idants/talilizers used
LIGHT Protection from light sources
N2 Under stream of oxygen yes/no
NEUTRAL Kind of neutralizing agents used
EREAG Extraction reagent(s)
E TIME Extraction duration
ECOMPL Completion critei ia ot extraction
SAPONIFIED Saponified samples yes/no
SREAG Sapon,fication reagent(,)
S TIME Saponification duration
STF NP Saponification temperature
PR MLIHOD Principle method emploed
CNR Chromatographic method number
LENGTH Length of HPLC column (mm)
DCOLUMN Diameter of IIP C column (mm)
STATPHASE Stationary phase
NAME Common/commercial name of the column
205
APPENDIX 2. (continued)
From food.dbf:
206
APPENDIX 2. (continued)
From method.dbf:
From dala.dbf
The data selected were combined by linking the three data tiles through the foodnr field
present in the food dbf and data dbf files and lie refnr field in the data dbf ard the
method dbf files The procedure used was as describcd in the d3ASE IV manual which
can be consulted if the data ame required in a diffetent tormat
207
APPENDIX 3 CHEMICAL STRUCTURE OF CAROTENOIDS
' -carotene'N N
a-carotene
P -carotene
y-carotene
2OH
-cryptoxanthin
209
APPENDIX 3 (continued)
HO /3-cryptoxanthin
HO lutein
lycopene
violaxanthin
OH
HO zeaxanithin
phytoene
phytofluene