PROBLEM

SET # 1: The cell

NAME: __________________________________________ YR & SECTION: _______DATE SUBMITTED:_____

1. List down 5 major differences between prokaryotic and eukaryotic cells.
2. Complete the table below.

Cell organelle Function/s Biochemical Prokaryotic Animal cell Plant cell
composition cell
1. Nucleus
2. Glyoxysome
3. Mitochondria
4. Cell wall
5. Cell membrance
6. Cytoplasm
7. Lysosome
8. Endoplasmic
reticulum
9. Chloroplast
10. Peroxisome
11. Flagella
12. Centrioles
13. Vacuoles
14. Ribosomes
15. Cilia
16. Golgi apparatus
17. Nucleolus
18. Cytoplasm

3. What are the three major steps in separating components of tissues and cells? Discuss
each briefly.
4. What are the different methods of cell lysis? Cite examples of each.
5. What is centrifugation? What factors determine the rate at which cell fractions can be
separated in the presence of a centrifugal field?
(a) If you centrifuge a cellular homogenate for a short time at low G-forces, what will the
pellet contain?
(b) If you centrifuge a cellular homogenate for a very long time at low G-forces, what will
the pellet contain?
6. After cell lysis and fractionation, what will be found in the crude fraction? What should be
found in the nuclear fraction? What should be found in the mitochondrial fraction? What
should be found in the soluble fraction?