3.
What
are
the
three
major
steps
in
separating
components
of
tissues
and
cells?
Discuss
each
briefly.
4.
What
are
the
different
methods
of
cell
lysis?
Cite
examples
of
each.
5.
What
is
centrifugation?
What
factors
determine
the
rate
at
which
cell
fractions
can
be
separated
in
the
presence
of
a
centrifugal
field?
(a)
If
you
centrifuge
a
cellular
homogenate
for
a
short
time
at
low
G-forces,
what
will
the
pellet
contain?
(b)
If
you
centrifuge
a
cellular
homogenate
for
a
very
long
time
at
low
G-forces,
what
will
the
pellet
contain?
6.
After
cell
lysis
and
fractionation,
what
will
be
found
in
the
crude
fraction?
What
should
be
found
in
the
nuclear
fraction?
What
should
be
found
in
the
mitochondrial
fraction?
What
should
be
found
in
the
soluble
fraction?