Bahan-Bahan Penelitian:

1. Semen Kerbau

2. Semen sapi Simental, Brahman, Limousin, Bali

3. Albumin Telur

4. Alkohol 70%

5. Pewarna eosin 10%

6. Kafein

7. Pengencer Andromed

8. Antibiotik penisilin streptomisin

9. Spritus

10. Kuning itik telur

11. Air kelapa hijau muda

12. NaCL 0,3%

13. Lubricant

14. Aquadest

Alat-Alat Penelitian

1. Vagina buatan

2. Tabung reaksi

3. Gelas ukur

4. Pipet volume

5. Pipet tetes

6. Sentrifuge

7. Termometer

8. Objek glass

9. Deck glass

10. Mikroskop
11. Spoit

12. Kontainer lengkap

13. Water bath

14. Filling shilling

15. Tabung erlenmeyer

16. Striver

17. Straw printing

18. Saringan

19. Kertas lakmus

20. Lemari es

21. Haemocytometer Improved Neubaueri