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  • 1/R0 Vs 1/ (Pyruvate) For Different (Oxamate)

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    John Lane
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    The document contains data from experiments measuring the initial rate (1/R0) of an enzymatic reaction with varying concentrations of substrate (1/[pyruvate]) and inhibitor (Oxamate). Linear regression lines are fitted to the data sets for different Oxamate concentrations. Higher Oxamate concentrations result in lower reaction rates and shallower slopes, indicating Oxamate is a competitive inhibitor that binds the enzyme's active site.

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    The document contains data from experiments measuring the initial rate (1/R0) of an enzymatic reaction with varying concentrations of substrate (1/[pyruvate]) and inhibitor (Oxamate). Linear regression lines are fitted to the data sets for different Oxamate concentrations. Higher Oxamate concentrations result in lower reaction rates and shallower slopes, indicating Oxamate is a competitive inhibitor that binds the enzyme's active site.

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    © All Rights Reserved
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    9 views4 pages

    1/R0 Vs 1/ (Pyruvate) For Different (Oxamate)

    Uploaded by

    John Lane
    The document contains data from experiments measuring the initial rate (1/R0) of an enzymatic reaction with varying concentrations of substrate (1/[pyruvate]) and inhibitor (Oxamate). Linear regression lines are fitted to the data sets for different Oxamate concentrations. Higher Oxamate concentrations result in lower reaction rates and shallower slopes, indicating Oxamate is a competitive inhibitor that binds the enzyme's active site.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 4

    1/pyru 1/r 1/R0 vs 1/[pyruvate] for

    250 34.60208
    300
    4 -0.0289 1041.667 37.59398
    250 f(x) = 0.0507282063x + 16.39027
    0.96 -0.0266 2631.579 68.02721
    0.38 -0.0147 4166.667 111.9821 200 f(x) = 0.0400019009x + 13.80369
    0.24 -0.00893 5000 119.7605 150

    1/R0
    0.2 -0.00835
    100 f(x) = 0.0196987097x + 22.82229

    50

    0
    0 1000 2000 3000 4000 5000 6000
    1/[pyruvate]

    1/R0 vs 1/[pyru
    250

    1/pyru 1/r 200 f(x) = 0.0400019009x + 13


    250 33.22259
    150
    4 -0.0301 1041.667 53.8503

    1/R0
    0.96 -0.01857 2631.579 108.6957 100
    0.38 -0.0092 4166.667 163.9882
    0.24 -0.006098 5000 232.8831 50

    0.2 -0.004294 0
    0 1000 2000 3000 400
    1/[pyruvate]

    1/R0 vs 1/[pyr
    300

    250 f(x) = 0.05072820

    1/pyru 1/r 200


    250 35.08772 150
    1/R0

    4 -0.0285 1041.667 66.22517


    100
    0.96 -0.0151 2631.579 140
    0.38 -0.0198 4166.667 235.8491 50

    0.24 -0.00424 5000 268.8172 0


    0 1000 2000 30
    0.2 -0.00372
    1/[pyru
    0
    0 1000 2000 30
    1/[pyru
    vs 1/[pyruvate] for different [Oxamate]

    = 0.0507282063x + 16.3902749037
    0mM [Oxamate]
    = 0.0400019009x + 13.8036903761 Li near (0mM [Oxamate])
    0.2mM [Oxa mate]
    Li near (0.2mM [Oxamate])
    = 0.0196987097x + 22.8222904896 0.4 mM [Oxamate]
    Li near (0.4 mM [Oxa mate])

    2000 3000 4000 5000 6000


    1/[pyruvate]

    1/R0 vs 1/[pyruvate] for 0.2mM [Oxamate]

    f(x) = 0.0400019009x + 13.8036903761

    Col umn E
    Li near (Col umn E)

    1000 2000 3000 4000 5000 6000


    1/[pyruvate]

    1/R0 vs 1/[pyruvate] for 0.4mM [Oxamate]


    300

    250 f(x) = 0.0507282063x + 16.3902749037

    200
    Col umn E
    150
    Linea r (Col umn E)
    100

    50

    0
    0 1000 2000 3000 4000 5000 6000
    1/[pyruvate]
    0
    0 1000 2000 3000 4000 5000 6000
    1/[pyruvate]

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