CC PHNG PHP PHN TCH VI SINH

1. K tn cc phng php nh gi mc khng khun ca vi khun i vi vi

khun ch th
- Phng php Agar Spot
- Phng php khuych tn trn a thch (c l thch)
nh gi kh nng i khng ca sinh khi vi khun kho st

- Phng php o c
2. Nu cc dng tan huyt ca vi khun? Trong cc dng , dng no mnh nht, v
sao?

C 3 dng tan huyt ca vi khun:

- Tan huyt hon ton (): vng tan huyt trong, r

- Tan huyt khng hon ton (): xung quanh v di khun lc chuyn c v c mu
khc
- Khng tan huyt (): hon ton khng tan huyt

Trong cc dng tan huyt, dng tan huyt hon ton () l mnh nht v tan huyt dng ny,
cc thnh phn trong mu: hng cu, bch cu, tiu cu v huyt tng u b ph v lm cho
mu khng th ng, cn dng tan huyt khng hon ton () ch ph v hng cu.

3. C my loi c t vi sinh vt? Phn bit cc loi c t ?

C 2 loi c t:

- Ni c t (endotoxin): l nhng c t nm bn trong t bo vi khun, ch c gii

phng ra bn ngoi khi t bo vi khun b ph hy, chng ch c sn sinh t cc vi
khun gram m
- Ngoi c t (enterotoxin): c t do vi khun sng tit ra mi trng, c vi khun gram
m v gram dng u c th sinh ngoi c t
4. Nu nguyn tc gy c ca c t theo c ch A-B?

c t theo c ch A-B gm 2 tiu n v: tiu n v A (to c lc) v tiu n v B

(gn ln receptor trn mng t bo vt ch). u tin, gn tiu n v B ln receptor trn mng t
bo vt ch, sau a tiu n v A vo bn trong t bo vt ch to c lc.

5. K tn cc phng php c kh nng nh lng c vi sinh vt trong mu?

- Phng php m trc tip:
m trc tip bng bung m hng cu
m trc tip bng bung m Breed
m trc tip bng knh hin vi quang hc
 - Phng php m khun lc:
m khun lc bng phng php pha long, a
m khun lc bng phng php cy trang
m khun lc bng phng php ng xon c
- Phng php mng lc
- Phng php MPN
- Phng php lai khun lc
6. phn ng PCR xy ra cn c thnh phn no? Thnh phn no quan trng
nht?

Cc thnh phn phn ng PCR xy ra:

- Mu nucleic acid (DNA)

- Buffer PCR
- MgCl2
- Nucleotide t do (dNTP)
- Primer
- Taq DNA polymerase

Thnh phn quan trng nht l MgCl2 v:

- Thiu MgCl2: hot ng ca Taq DNA polymerase km dn n hiu sut PCR thp
- Tha MgCl2: thu nhn mt s sn phm khng c hiu, tnh chnh xc thp.
7. Nu cc yu cu khi tin hnh thu mu thc phm phn tch vi sinh
- Mu ly phi i din c cho l hng thc phm
- Nhn dng c r rang
- Hm lng cc cht hay cc vi sinh vt cn xc nh khng bin i k t lc ly mu
n khi phn tch
- Khi thu mu theo dy chuyn sn xut cn thu 3 cng on: cng on x l nguyn
liu, cng on bn thnh phm ( cng on ny nn ly mu ca 2 cng on lin tip
nhau) v cng on thnh phm.
- Thu mu v sinh cng nghip: dng c, bo h lao ng ca cng nhn nh gng tay,
ym, ng,, cc my mc trc tip sn xut.
8. Trong mi trng TSI cha cc ngun ng no? Mc ch ca th nghim trn
TSI l g?
- Mi trng TSI gm: 0.1% glucose, 1% lactose v 1% sucrose
- Mc ch ca th nghim trn mi trng TSI l xc nh kh nng s dng cc ngun
carbohydrate c mt trong mi trng, kh nng sinh hi v kh nng sinh H2S trong mi
trng ca vi sinh vt
9. phn ng pht quang sinh hc xy ra cn c nhng yu t no?

Cc yu t tham gia: c cht luciferin, enzyme luciferase, O2 v ATP

10. xc nh c s hin din ca E.coli trong mu cn th nghim sinh ha no,
Kt qu ra sao?

Nu kt qu ca th nghim IMiC nh sau th kt lun c s hin din ca E.coli trong mu

cn th nghim:

- Th nghim Indole: (+)

- Th nghim Methyl Red: (+)
- Th nghim Voges Proskauer: (-)
- Th nghim Citrate: (-)