Materials and Methods

Cimex lectularius, Amblyomma americanum, and Dermacentor variabilis

Materials
Small coolers (about 11.81 x 8.27 x 10.63 inches each)
Dry ice
1m2 pieces of white cloth
Forceps
Medium sized test tubes
80% ethanol

Methods
To make a dry ice trap:
Install a dry ice trap by taking approximately 3.5 lbs of dry ice and transporting it to the cooler.
Leave the lid open. Then, place underneath the cooler a 1m2 piece of white cloth. The trap
should resemble Figure 1. Leave trap to set overnight. Next morning at 8:00 a.m., collect the
species with a pair of forceps, and place it into a vial of 80% ethanol. Replace the used cloth
with a clean cloth and replace the dry ice, and let the trap set over night again. Repeat this
process for every dry ice trap set up.

Figure 1. Diagram of a dry ice trap

Place an ice trap in these locations across the United States, as shown in Figure 2:
1200 16th St. NW, Washington D.C. 20036 (Jefferson Hotel)
Place one dry ice trap in every room in the hotel, private and public.
130 W 46th St, New York, NY 10036 (Muse Hotel)
Place one dry ice trap in every room in the hotel, private and public.
2002 Cobblestone Ln, Bryan, TX 77807 (Ariannas House)
Place one in each bedroom in the house, the living room, and the laundry room
1130 N Westfield St, Oshkosh, WI 54902 (Evergreen Retirement Community)
Place one in each room, private and public
Figure 2. A map of the traps that should be placed