Scribd Logo
Upload

Welcome to Scribd!

  • Patent 2

    Uploaded by

    arip
    2 views21 pages
    US Patents

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    US Patents

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF or read online from Scribd
    Flag for inappropriate content
    2 views21 pages

    Patent 2

    Uploaded by

    arip
    US Patents

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF or read online from Scribd
    Flag for inappropriate content
    of 21
    ‘Method for producing triacetin from tobacco Us s010399 82 ABSTRACT ‘A marosor posing tiazein fom one emer prts of gems Nectarais ‘ced. The nan can bere a ern Mean pris bese (ror som cenan erase aceenproauea oy encores tgyeenn geantaa ny hyn ot aryireabcortanng sang men ete om obaeo Boma or eee an tes alo deed om ‘era oonass or eee Te rerspn ako provses anaes ad omeastons ‘elu ecco wile ance composts lice iacebn oats nomen o mare ee gerue Norra. DESCRIPTION “Thepresrtimenton ees to crosucs rade crcenves fate noe gery ede for any Hera dee fm ary oe or nce ses of ase Nozres or ma eerwes reoporst oe, ens [prhleredorhumen consimpion Opec tes merged or empanerts ened ned Yom eto patos of sn ane Newbara sees eeptarsraing cles schon conte, fave a ustatay Seca raped vite naineuoe shee, a er colon osmosis suche shredsedtebexzo eg cut rom) sounded bye paper ‘wae: ey ominga scaled baer Nmay a gee ha Cyrene ter rer alga an nd ra elnorano wan 6839 iumatbad oy permet ian oe oe ao” Caran ret Ireapartoaeclsmertnovng mute ogres, erd ons of 28 segretiscanconprse acid chacen prices Typical he le era laeesehostoone ara err oezeo rod ang a eetumeeEng wepzNg rien irom astern facets hos terome ceed paras he "poate are aug wp oe oie on of araun maieveam tafe wih ember‘ or ogre w rps smoker by Ig ors sts ees mec baring hese The act he cen aren ‘ako ihr mah by dango he ope ee 60 ro mecpeete ‘The ceo aed or cae mantra ended am Fr cxanos, conan copuarcescze bane, cormony fSNea 3 8 “Ameren bids compsce ries cf frre bao, te tba, Ott tao, on ary ase, cen poreses s,s ‘erent nares one cess anerso sens The ese ara ach ipe fitout nce He ned the arf 2 pare carcas tse ares Wom bar trend HoAcye oman totes blenes, here onco males tp aetna fe ‘eg he Ont osceo mes ws ees poprtn othe en See, exempt, Tserc Encepea Opes (ESP 4245("S64) ‘Brone, Te Desf Coneres, ES» 3 (180) on Tabacco Proucion Chemis nd Tetncogy, Dav et a (Es) p. 34853, ‘Tough be jer, ars teste! mts rd ssbves tov been recess er atemgire oor racer a netocicstse meer Ica Intec rcs Fat example mide ot eater o:enies hav een tees nora ates enmier ar corer popes af ne tae!2 tena, or the cas of stale foaecoratenl Yo erne serio ‘esewon trber Fiagase ayaa Protyarie@ ar 27 20H) COnpminccgees —.Rayraae Tce Carseny Goocistos seek Entice even rr Sin Nena nes), 2). [stra Lites USPTO, USPTO angen Cssceet CLAS =) + Ametned tr roauing teen fom oe or mre nsf anus Notas ee rats eee om cs or mere plans ogous Mecens wth sconpceton Ccarprangsn al rial een nine anh cade ore pr oti oa eter spt ne eueieta est some of ‘ne aeygyezrlcortaned nthe statrg meters be hotles techy rotcing a terete comping ct (srg ein fam ie Iremeausscovereng ger te fom af a enn are) sjesng a sane cortaning seca teang rte densa one re plats of gre Neataraio stuns atom ety pong = Iremeaareconerng artes str cee ec TM RE inemedate compan acta afar anes ae react ©) ‘coreang asm mo eases oeom scat a Susy ors ison aoe eat uneroncbars avon eeesen, Perey ipods asain Theo okt 1, rein te ssa centring send ‘surg macal strirg mcrae cone te tte:0 2 Tremetnad oto 2 when te aca contring sero ‘rng melded Ham ose tomes. 4-trametad otto 1, wrote conaons avomgcatteston comonseconacting each ecan weh ansbon etl de {5 Theretod ol 4 herein heen ele ‘seid Bom We group cvs 203 WOs, TOs Ms 6. Tre metadata 1, wr Re conatons avon catseston ower se ocr wn coe srs ue 9: Themetod tom sarin te werton mat 20ers {9 Therein otto 6 ahr te tanston met oe YD, 10. Theatr of aim tere the anion ret oes TO 1 Therebodoci ten anton mel ote MDs, 12 Amatostor roaueng Hata Non 76 a mots dat ot gene Iwaxtarn teretodcomersng 2) certain an abyoyezckcrtnng Fs ‘statng marl ded Yom one or mate ans af ers ote Wh = seror popes naraten sree gerne song we ‘ewan Detonaeo ata. The sens aut cfcgaete sate canbe certeresby icerporsingtovng mater ino vais conprerso¢ tee seni Nevatg ses ude ert aa act aterareacors seh as preanes artes eto compas. ‘Sea Lefinget el, Tears Pag hr Song Pest, Rl ‘Roy ote2e Caneeny (572. nce weerzesns ern FACS Insc cases eaten proceses mln Hes of eat cama the processed abate a esed abt ovulate deed cesar opates,oracesredphysca nee ort Yrows pests er eptng avo and sors cargo fr use ntbececorpoetons to Ranets tat US. Pa ie SUTEsI9.0 Wwe US. Pa. ‘505850 fo thte was US Pat No 5725552 > Seraabugh J US Pak 5201486 9Raymons ea US Pal No. 625625 9 Coeran iets. US Pat No 622508 Caeman ets Ate, S828 2439 Colerar,ilsiat US. Pat No. 41022310 Dubeetal- US. PEL ND. (a5 409 cena Hara US Pak ho GSO. Se UME tA LSP ‘sl Puno 52417926 fCaeman i aU epee ‘No.1. 751 Coleen ital. Nos Aug. 14.2008 sen amen a ‘enpertadersnby ference Aina eres epemeraive covets hat es be redo 5 alec na a ucts in cbceo Drotiris oe stin POT YO 7OHMEDD Loos uhm eos etn eres. ‘ant:a ao ay to enoyeam 2cocate ‘anatlze om. Pane) pepsi isopods ae ngoyed yen are an rxcaed abet o oa oman eraton phe mau 6 wee otous pes of oteesstacrzo edicts a seteth NUS Fat No ssesssoSerwats US PH ho 32982:7U La US. PaL ND 651375 toprvenstal Us. Pet No 4S780030 SaneateUR ee: US. Pat No. 624209 Sty etal: US Pat No. #957 £07 Tonnsere US Pat Ns 50523521 Sper, Met a and US Pat Na. 558741 hte ta US Pa Acpl Pub. oe. 205024452 Suid tt 208019673009 Engevom a: ard 209902925501 urate: PCT NO CATEEID ‘Aram et POT WO OSOS00E0 fo they ea PCT WO OSOHEOSS 0 Dhl ard PCT WO CSCLIOSS to itr a a, ech of within meororsts evan rtenee. Se, rea, yp of oroiiess ‘obec fomutons ingress, ons processing netatlopes seth a US Pat Ko 65304D%o ey otal ant US Pet No 7.03200! a hiey ta ezznctuncn ncoparata ren yer, ‘crete of emotes tebaco protec 0 anu Repesectre ‘ype oat rut reas commen rear» ase "rar oer rare Sucpe prc n Ses, rough compares ch | Sesh Mah 8, Fad Lunde A Gusts AS Seana Teoslekomoagn AS, ard Roce Produzten AB Sue pod Sate ‘SU SA hoo tes mariesesunder ne Paenanes Camel Svs Fos hctoste,orireracdl ora eserese fra pero ee scent ior et leasteome oft sevaheetl orsiesin tbe tn meal De rotons, oe sung en moma corereng aye) eoeeng ein fom therlemedate carting gan nfm ale seo Feet) oubeeag n Ste orton eens aT Maar mse fram one rare pa oigens Nectaeis sachin deson Se osug sn wsiredalconprsing see a (eng ete ad 1 tne miemetine comers sce eds farm ar isberes acer acest (conse a usa of ne od cen eater 2 ety ofthe looses ae 2 ear ud ere emg ear eo?, wsrom ME nto fing exerieaencomarae crating ach ica he enanon renee, ers meso. 14 Themsina fam 2 when ne onson met ass Os 0 17 hat race Hct fa ecco Haas de ret corprang:()caratrg a cynsortconanng mat earng mora ryotodse sn ase sem a roads = meals oan ease fora peod tome arent ‘or sles seme ote atl cetanesin ne sara mr beytoyaee, tee lod nema cerprang smn) easeg earn fon is esmaaIs coTETENg yenin tof nce et renin) ection awe ‘entra s:cra strana masa conve Yom:nber > Hemas0 "0 SEES Citeon, rere procuring en ifemesietecomotsing arte 2c) Inked ete see ee) onan qunty fe ole yee reasarwin 2 ue of 6 ease cee an rect under corns favong exterizaton, wren te corse cag exeestoncomere vkaing ech ear ith nari tal ni, ny prc eat mettre caine ch oe or ede, ‘2 Themetod ol 17, heen he compaston corpses (Camel Sn Crt ac Care! Se Spice by Rey Tebce Campane ab for earl, raga, {NIG0D Li Oye Assesment Compara Le Cc Assessor of Gaeta Lace an Pater Sus 225). n ‘ssaon, sora quai sncarezeoatsa wth aus marwecure Pao Been ssorekd eb @ 2 Elo GtnaTok ‘Sencar, Represent svat oc: procs exo rave beer meres unr he redenames Cher Tay House of Ober Tat 5, Copeaaoen, Sioa, StoaDhy Roose Red Seat Huby end Reel US. Sarees Teos:to Ca: Eb oy Fp Nore USA: Lo Gane. Pen, Tars Pace Keak, House Yangoen, z= Dera Kenly Kg, en Marat Cave by Corwcos Corea LLC; ard Carel ibs, Care! es, ns Coma Supety Rd Repos Teas Company Tre sensory eves fsck boc can be enhanedby eeu of oan ave maenls See, or ramp, US Pat Spe Pls Now 20020122562 hes 2ECQOHE2EE3 Wins, 208072627 ee fal: 20070186941 to Hotton, Jr etal: 2007/0186942 to Sricane eal: 2008/008110 to Dube tal: 2008100281160 FRobirson al; 2008/0028117 to Mus el; 2006/0173317 to RooInson el; and Z00B/0Z02SE6 0 Nelsen et al, each of hich is incorporated herein by reference. Found as an ingredient in any of@rumber f tobacco prec! fermuatons i sen (CAS 192-751) Teta is also ‘known, for example 8s 1.2 topanetl tiscette alycery acetate: Pecetlolyenne or alycercum inacstes While it may serve any ofa number ef furctonsin tobacco podustforrulatons, tice is known inthe ato serveas 2 humectart, a plascizer, ora schent. In adciton tesein may tuncion 2s futive ran antfungal A qu at ypica oomtempereute, aren is coloress and viscous. “Tiacetin i usetul 28a component of svat oftebscco products of asm ingresent inte prozessng oftobaee9. See, for example, U.S. Pet No. 3,165,978 ioParmele et a; US. Pat No. 4.715.200 to Nichols tl; US. Pst. No, 5,038,202 to White tal: US. Pat No, 7.237.585 to Clark et al: and US. Pat No, 7.793.865 toDute etal. each of whe is Incorporsiea hereby refeence, {Ast chou be clear from the feegoing that triacetin ie sefulinthe formulation of vaioustobaezo produsts, it can alo be ‘en tatit woul accercnaly te desirable t provide @ metnod for producing tiacelin fom obarco, thats. in articular fom Nicotine species, for use, inter ai, in tebecco compestins ulizedin a vtiety of tobacco products or inthe prosessing of nbseco. SUMMARY OF THE INVENTION “The present invention provides materials ffom Nctiana species (eg, tebacco-derived materials) comprsing isolated comporont fom plana ofths Nctianeepecice weet forinzorporation rte tobscz0 compoctins utitzsdin avait of tobacco products, such as smoking articles and smokeless tobecco products. The invetion aso povides methocs for 'solatng components fom Moctena species (29, tebeeco material), and meineds for processing these components nd tobacze mettials incorporating those componarta. For example, basco derived metariele canbe prepare by eubjecting ai leesta pation of tobacco plant (ea, eves. sisks, rots or sters)to a separation process, which tysicall can Incluge malspiesequertil recon steps, in ode io iolate desired components othe tovstco mst ‘hon usedin connection wth te invention, the term “acyleycerc denctes ary one or mors ofthe folowing ‘monoacylglycera; iyiglyerot,triaryllycerol \Wen used in connection with he invention. the term “biomass” derotes one cr more potions cf @ plant, endin particular denotes substantially the entity ofthe superterranean portion c's plant, optional including some orl ofthe subterranean pation oi plant. Accorainaly the term ‘biomass” may refer‘ leaf orto seed orto any ther superteranean potion of plat, oro any combination thereot, optionally insiucing some or all ofthe subterarean perten of plant \Wen used in connection with the invention, the term ‘one or mere plants cf genus Neotans" dencies any one or more plenis of he gerus Ncotena of fami Solanaceee, inziucing, for example, any one or more of fhe following: N. sate, N. rents, N. excesir, N forgetana, N. gauce, NV. gutinosa, N. gosse, I. Kawekamu, N. mighuena, N.langscor, topnora, NV. setchell, W syvestns, W. fomentosa,N.fomentostorms, N. undulas, and N. x sanderse, N. arrears, W. anplexicauls, N Benavides, Donsniensis, N. debneyi,N. longiiors, N. martina, A. megsiosahon, N.cocidertalis, N ‘paniculsta, N. pumbaginflia, N.rsimordl,N.rosulats, N. rustica, N. simulans, N. stockoni, N. suaveolens, N.‘abscum, 1N.umbratics,N.veutina, and N.wigancioices, N. aceuls, N.scumnats, N atenuata, N benthamisns, N cavicola, N cleveland, N. cordfoli, N. corymbosa, N. ragrans, N. goodspeedii N.finesrs, N.iersi, N. nudicauts, N cbtusiota, N. ‘ocuentsls subsp. Herspers, N. paver, N_petuniides, N. uadrivalvs, N.reparda, N.rotundfoli, N Solano, N. ‘spegezzinit \Wmnen used in connection wih the inventon,“akall metal nycroxde" denotes any one or more cf he compounds having forma MOH where MIS L, Na, K, FD, C5, oF Fr \When uesd in connscton with he invenon,“aksine sath oxide or hydroxide’ denotes any ons or mers of he compounds having formula MO or MIOH)z where Mis Be, Mg, Ca, Sr, Ba, oF Ra. Accordingly, for example, when used in ‘connection withthe invention, “contacting an atylaycerol-zortaining fst starting material derved tom one or mote plerts| ‘of gerus Nicotiana andor ftom tobacco biomass with 8 compostion comprising an alkaline earth ride or hycroxide” denotes coniacing an scyiglyceroL-containing fst staring material derived from one or more plants of gerus Nictisns andor fram iobecce biomass with any one or more ofthe compounds Faving formula MO or M(OH} where Mis Be, Mg, Ce, 8: Ba, ore \When used in connection with he invention, “ineral sci’ denotes an norgaric acid and accordingly can refer, for ‘example, to any one or mere ofthe following: sufi acid phosphoric acid nit acd: chlor aid: hydrofuotc ec: hnydrechiore act; hydrobromé atid: hycroiodie acd; chromic acid; sulfrous acid phosphorous acid nitrous aci¢; a hhalogensulfonc acid HSO3X wherein Xis halogen: perchloric atic: erbromic acid: peice act: hydrogen sulfce: Inyposhosphorous acc; tezefuorobare acd; hexaflucraphosoharic acid. Accordingly, for example, when used in Connection withthe inveriton, "cic conditions" denctes, intr ala, conditons obtained by contacting compostion, such 88 eactart, with @ mineral acie, such as concentrated suture acid, and denotes more generally conditions ungersiood by 8 person sklegin the at be eae conalions:conations In whieh pH is aca, at, ess than 7, rather tran ‘akaline, that is, greater than 7. Vinen usedin conection wth the invention, “ransiion metal vide" Genotes, inter aa, 202, Ws, TIOp, oF MoOs, end denotes more generally 8 compostion understood by a gersonskiledin ne arto be @ transtion metaloxde, \When used in conrecton ith the invention, “esterase” denctes any one or more enzymes catalyzing hycrlysis of an esterlinkage. For exarmple, “esterase can refer to any one or mare enzymes belonging to EC 3. (enzymes scting on ester bonds, including, n paicular any one or more enzymes belonging o EC 3.1.1 (carboxyle ester hydrolases). “Esterase” can sccorcingly reer, forexempe, to any one or more enzymes belonging toany ofthe following: EC3.1.1.1 (carboxylesterases), EC 31.13 (riaryllycerollipases), EC 3.1.1.6 (actylesterases} EC 3 1.123 (ecylgleero pases} “The use of Miccians-cenved(e 9, tobs:co-ceives) materials othe present invention enables the preparation o tovac:0 composiions for smoking atles or smokeless tobac:o compositions that are deed subsiantally oreven entirely trom [Neotiana mateals. Fer example, a iobecco compostion can incorgoretetobecze or tobacco-derived material ofsome form, including solsted components from Nicotiana species, such that atleast about 80 weight pee, more typically at Jesstsbout 90 weight percent, or even at lest about 95 weight percent (ona dry weight basis), of hat tobacco composition consisis cf tobaczo-derived mater. nas been recagnzec that theres @ need to make fuller use of metenl o sunstance fam tobacco, andin partcule rom plans or potions cf plats from the Nicotians species. Readly avaiable stating materials or inputs from plants or partons Cofplens from the Nicotians species, such staring materials or inpu's being useful in paricular for clusicn as starting ‘materials orinputs in 3 process uhe‘eby material or substance fom tobacro can be mor fly ulzed induce inter lis, tobacco biomass and toberco seed. Tobacco biomass can include for exemple the entirety ofthe substance of atotacco plantthat hes been harvested whole Tobacco biomass can include for exami essentelly all fhe supertertaneen parts (of tobecco plant and optonaly can include some oral ofthe subterranean parts ofa tobaco plant. Tobacco biomass cn include for example the sold potion of tobacco pant that has been harvested whole, o the so poston of cessentaly ll of superterrancan pars of e tobacco plat, anc from which so-saled “green juice" hes been expelled for ‘example through the action ofa screw press. Tobacco biomass cen include for exemple such 8 sold potion from which st least 8 potion of he water has been removed by drying. A tobacco seed may o:eupy only very smal vole, suchas fraction fe cubie ilimeior. For hie reason, ito typiclly pacteel to collect glurlty of tobacco sesde wnon itis chosen tonanvesttpecco seed, Among ways in which fuller use can be made of material er substance fom tobacco, and in parcular rom plants or portions of plants from the Nicotiana species. ae vaious chemical transformations to which rlants orprtons of plan's ‘fom the Mcotiane saevies con be subjected Such chemizaltranaforetions may result in outputs or products having one cr more cested or fvorable properties. Such outputs cr products may themselves be usetl as stertng material or inputs for further usetl processes. Among chemical transformations to which slents or pation of pans fom the Neotsna epotios canbe eubjctod ara hycrolyce, including or example, ecié-catelyzod hydralyses and bese-cctalyzod rnyaroyses. cic-stayzea nycroyses of esters and base-ceialyzed nycroyses of esters ste known inthe ait An ester which may be subjest io hycrolss includes an ester found a the sn-1, s.2 or sn poston of tacyldycero, of 8 acylyeerol, oof = monoacylpyeera, such 5, in paculr, « tiscylyeera,diseylalycerol or manoscylglyceral present ‘n 0: derives from a tobacco plant or ary potion thereat, nludng, for exam, acco biomass or @ tbercD Seed. \arious embociments of hycrolyses of esters, nclucing acidzatalzed hycrolyses of esters and bese-cetalyzed nyardyses: fester, are Inonninthe at An sid sutable for an avc-cstalyzed hydrolysis of anester, such as en ester'n = tiaeyllyceral a daeylalycerl, ora moncacylelyeorl, may bo mineral avi. bese eutcble for « bese-catlycod ryrotjts ofan ester, such as an esierina macyHcero|, @.aleeyQH/Cero, cr @ moncatylelyeerol, may d€ @ hyorodde of ‘2 G0up f skal metal monovalent cation, such as Ithium hydroxie, sodium hydroxide, or potassium hydroxide. tis known in the at thatthe intermesty metabotsm cf cent. icluira, inter ala, lants of genus Nicotins, produces ‘eretate. Sea, for sxarple, Hockn stal (1083), American JourralofSctary, Va. 40, Ne. 7, pp. 502 f Tho gest moiatyia presere within ne ubquttous ntermeciateacety coenzyme, which patlpstes ina ney of metatob restions. -Accorcinaly, acetate may ready be prepsred ffom aplantscurce, suchas rom. plant source fom any ofthe Nicotiana species. ‘Conattons for concersation fesctons of polynyroxy alcohols such 2s glycerin with acts suchas scele acid ave known in the att See, fer example, Geloss etal 2003), Inustial and Engineering Chemical Research, Vol 42, No 25,9p 6526 1 Ferrata eta. (2008), Catalysis Communications, Vo. 10, Wo. §, pp. 481; Lu sta. (2011), Green Chemisty, Vol. 13, No.3, pp. 697. In an aspect, tne invention provides a tobe: co compostion fr sein a smoking arte ora smokeless tobacco composition cempreing a ‘ota:eo material and a triceetn-cortaining compenent derived fom bicmase ore eed af the [Nicofara species, wherein the triaetn-contining component comprises triacetin, In cotain ambocimerta, a glierin-contsning component arcordng fo the invenin ie formed using techniquos adaoted | ‘orexpressinglicids flo biomass or seed, such ss ich pressure squeezina or cod pressing. Allemaively, ¢ dycern- contsining component a:cordig fo the invention is formed by extractng conponerts fom biomass or seed using ‘opropiste erection techniaues and selven's. Exemplary solvents include hydocarbons such as heptane and hexsne. tte: separation processes can be used, such as chromslagiopy,csillation,firtion, recrystalizaio,solvet-solent patitoning, and combinations thereof A glycefn-sonaiing component formed using an extraction process can be either {he soWert-soltle pation othe insoluble resiue of biomess Sees meter remahing ar savers exiacton. A lyeerr-containing component formed using a pressing process may be ine ali pc.conining pertion of biomass oF ‘sea expressed fom presses biomass or seed mater. In cetsin ambacimarta, an acetate containing component aecorcing tothe nvertin ie formad using sisillation techiquss ‘adapted for obsaning acete 20 om bosass or ore or more anetomisal pars of plant. Atematnely, an azetate- ‘ontzinng eampanent a2carding tthe invention is formed by extractng componente frm biomass or one or ore ‘antomical parts of pnt using eppropite ecrecton techniques and solvents. Other separation prozesses canbe used, suchas chromatography, flraton, erystalizaon, slventeovent paritoning, ané combinations thereof. An ezetaie~ contsining component formed using an extraction rovess can be sithe the scvent-saluble potion othe insoluble rescue of ticmass or other plant material remsining ater scent extraction. | gyserin-coniining component according tothe invention comprises glycerin, usefl forthe rofucton of tacetin. LUkewise, an aoetate- treatment and combinations of such steps In apretered embodiment, bicelir-cortaiing component according te the ivertion is made from ghceri-cortaning ccomporent derives from tiomass or one or mere anatomical parts oftobareo and an acetate-cortaining component cemvea tom biomess or one or more anstomical pats ef bacco, “The invertion alsa proves amoking rela and amckelesetebesce compositions tht include srscetin sontsining comporent as described herein. For example, a tobacco composition can incorporetea rsceti-contaring component within a cesing fermulaion cra top dressing formulation copied fotobarco stip oras acompanent of arecenstiuted tobeccomaterial “The invetion, nan espec, relates to @ method for prepaing ¢ blavetn-contning cemponent fom biomess o one or mere arstomicsl parts of the Nicotiana species. In paticular preferred embodiment, amethod for preparing = tetetin- contaning component eccorcing tothe invention comprises 1 islatng an acylgyterol-contsinrg freon flom harvestec iomass or Sood of the Nisotians apesice by eubjocting the harvested biomase or sped ora portion theeo! to cold ‘pressing, solvent edracion, chromatocranhy disilation, fitaton, recystallzation, sovent-solvent aerttoning oF 3 combination theree to form an isolated acyylycerd-cortaning faction; (2 chemically transforming the 2c /alyseo containing faction by subjecting the aylgyceraLcentaining fraction to rycryss thereby forming @ glyeern-containirg ‘omporent (3) isoistng an acstete-contaning component om harvested bomass or one oF ore anatomical pars ct ne ‘Micotara apotos by aubjecing he harvested biomase or ore or mors anctorical pate or aporton thereof io sald pressing, solvent edracton, chromatocraphy disilation, fitaton, recrystallzaton, svent-solvent gerttoning, of 3 ‘combination therec fo farm an islated acetat-cortsning component 4) reacting the ghyeerr-cortaining campenert with the acetate

    You might also like