Genome Annotation- It includes assigning the function to the unknown/genome sequence, for

coding and non-coding part. Genome Annotatiob requires Structure Annotation and Functional
annotation.

Software for Gene Annotation

for prokaryotes-- Glimmer, ChemGenome, Genemark.

for eukaryoyes-- Genscan, Ab initio, AUGUSTUS

Hidden Markov Module

Hidden Markov Model (HMM) is a Markov model in which the system being modeled is assumed
to be a process with unobserved hidden states. The possibility of the future process is
calculated. Accuracy rate is 2/100.

Physicochemical Properties on which these softwares are based upon---

Solvation, Hydrogen bonding, and Stacking

Easygene- Rvalue cutoff---> <2 (predicted gene) otherwise not.

Points to keep in mind.

If you have a large sequence then you must have a large cutoff value and same in the case of
small gene.

Venny Statistical Tool can be used to create Venn Diagram out of exclusive gene queries from
different softwares, and then non similar can be BLASTn'ed or BLASTp'ed afterwards to calcultae
the dissimilar portions. The portion has the possibility of being a novel gene.