Fantastic Properties

of Nano-bubbles

Kaneo Chiba
Masayoshi Takahashi
 Burstatthesurface

Disappear
Goesup
rapidly Continue
Shrinking

Ordinarybubble Microbubble Nanobubble

(Macrobubble)

 Nano-bubble production by collapsing microbubble

Waste water treatment

OH
Microbubble

Decomposition
Collapse of organic chemicals

OH Radical generation
Physical stimulus

Nanobubble production with salt (electrolytes)

Microbubble

Collapse
Nano-bubble
Physical stimulus

 Nono-bubble generator

Microbubble
Nano-bubble

Physical stimulus

Analysis of nano-bubble by Dynamic light scattering (DLS)

20
18
16
14
12
10
8
6
4
2
0
76 93 113 138 168 204 249 304 370

Particle size (nm)

The deference of size between MB and NB

Microbubble 10 30m

Nano-bubble
< 200nm

 The deference of life time between MB and NB

Disappear
Continue
Shrinking

Microbubble Nanobubble

Several minutes Several months

Why ?
 Electrode Light Electrode
(+) ( +)

Water + microbubble
Electrophoresis cell

Microscope

Movement of microbubbles in electrophoresis cell

Change in potential of microbubble over time

time Ions

Ions Microbubble
Accumulation of surrounding ions
-80
with salt (electrolytes)
-70
potential mV

-60
time
-50

-40

-30

-20
0 5 10 15 20 25 30

Diameter m
 Stabilization mechanism of nanobubble (assumption)

Nano-bubble
Negatively charged

Surrounded by accumulated ions

Aqueous solution (counter ions)
Salting out

Prevention of gas dispersion

Nano-bubble generator

 Normal ozone water vs. Ozone nano-bubble

Ozone water ; disinfection & sterilizing Food industry, medical

Normal method

Ozone 1.5mg/L 0.1mg/L

Bubbling 1 hour

Nano-bubble

Collapse
More than
1.5mg/L
1.0mg/L

Micro-bubble Nano-bubble 100 days 13

 How to treat the problem of norovirus

Norovirus During
cultivation

Norovirus symptoms:
vomiting, diarrhea, Oyster
a low-grade fever, chills,
headache, muscle aches
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 Conventional method

Oyster
Chlorine

Chlorine ( high concentration )

Norovirus ----- not good for foods

Ozone water
Oyster
Ozone = strong oxidation ability

Virus inactivation
Norovirus 15
Oysters in ozone nanobuble water for 1 day

16
Before ozone nanobubble treatment

17
 After ozone nanobubble treatment

More than 99% of feline calicivirus in the body --- inactivated

18
Practical method for inactivation of norovirus by ozone nano-bubble

19
 Active effect for fishes

Carps : recovering from Carp herpes ( serious disease )

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Oxidation ability of ozone nano-bubble as a form of mist
21
more than 80% of original oxidation ability

reduction of smell and bacteria from atmospheres

22
 Oxygen nanobubble

Collapse of oxygen microbubble About 1% salt

Recovery of weakened fishes

Active effect for creatures

23
Nano-Bubble Aquarium 24
Astonishing effects on plant

Orchid in oxygen NB water

for more than 1 month

Still alive

25
 Initial growth of plants

White radish White radish

Broccoli Broccoli

Tap water Oxygen nano-bubble water

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 Active effect for oysters

Room temperature

-20 for 1 day in oxygen NB

Still alive

After 1 hour

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 Application of nanobubble in medicine

Prevention of arteriosclerosis (hardening of blood vessel )

Oxygen nanobubble inhibits mRNA expression induced by
TNF in RAOECs

ICAM1

VCAM1
Heart attack

GAPDH Stroke

control TNF control TNF

alpha alpha

With oxygen
Culture aorta endothelium of rat Department of Cardiology,
nanobubble
Jichi Medical University
Cytokine stimulationTNF alpha
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 Application of nanobubble in medicine

Preservation of living tissue / organ

Vagus nerve of rat : after 1 week

Isotonic NaCl solution Oxygen nanobubble

Tokyo Medical and Dental University

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Thank you very much
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