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Hormonal Content of Pollen Grains Forselected Strains of Date Palm (Phoenix Dactylifera L.) and TheirInfluence On Characteristics of Productivity Forbarhi and Sayer Cultivars
Hormonal Content of Pollen Grains Forselected Strains of Date Palm (Phoenix Dactylifera L.) and TheirInfluence On Characteristics of Productivity Forbarhi and Sayer Cultivars
( ) Phoenix dactylifera L.
.
- -
- . =
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5
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9 6
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( ) 74 69 64
5
( Phoenixdactylifera
) L.
( 5)4 ) Arecaceae (Palmaceae
Palmae 644
4000
Phoenix
5
)6:( Nixon 5)5(dactylifera
)31(Swingle
Metaxenia
Fard No.4
Mosque Al-
)59( GhnaimMuhtaseb
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Al- Ghnaim
5
)5=( Muhtaseb
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( )6< 68 67 6459
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5HPLC
)IAA(Indol -3- acetic acid
)6< 6; 5: 59(
)GA3( Gibbrellic acid
6- Furfuryl amino purine
)ABA ()Kinetine
)Abscisic acid
(5)2
( 5 )12
-5 1.0 5
-6 50 %80
48 59
-7
( )
-8
Rotary flash evaporator
30 %20 (5)7
-9 60
5
Deionized distelled
water
-: Lead >
acetate %40
5%22 5
86
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9 ; -
5 Centerfuge 12
3000/
< - 60
( )HPLC
5
Liquid Chromatography
Shimadzu Shimadzu
( )pH 2.5
( )HCl
( 5(6 Diethyl
HPLC ether
Retention time Area
5 30
( )Column2504.6 I.D (C-18 15
)mm Absolute methyl
( V/V )mobilephase ( IAA alcohol
) Kinetin ABA GA3 45 5
B( (Acetonitrile >A )
1mM >methanol1%acetic acid + 5
tetrabutylamonum phosphate+400
)ltryethylamine ( )pH <5: %5
/ 1 NaOH
( )6<4 nm Ethyl
74 54 acetate
Injector 5Rheodyn-712
> 15
=
87
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-
5 6458 6457
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(
6458/7/6: 6457/8/:
)
( 5 =)
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(<)
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= )54
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54
6458 6457
)6=( Furr Ream
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= 544
+
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)6=( Furr Ream
5
= 544
+
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>-
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= 544
()
(< /)5
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/
9 8 ( )
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Randomized complete
( )GA3 6 9 block design
545:== 545<;9 /
: 65=59
5 /
;5=;5 ) ( Genstat 12thedition
/ (
7 L.S.D. ) Least Significant
: Differences 5)7( %9
5
( )5
(
)
=
( )IAA = 958<: 9579 /
8 < 456:
/ 5
957;:
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)55(
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9
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5
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)65( Iqbal
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/
Al- Dhakki Shakri
)59( GhnaimMuhtaseb
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. 6 7 8 9;
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=
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4
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Mustafa )69(
-2
Amhat
Aswan ( )2
.
3
()%31.15
. 1 (( )%31.93
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.
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.
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7
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ABA
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=8
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.
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8 .
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=
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-9 ()
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( 99.67 97.18
96.53)
95
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6457
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%9L.S.D
6458
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5 644<5 -5
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5 .
-6 . 1998.
99
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= - .2111. - .
-7
( )Phoenix dactylifera L. .2111.
( ) .
. 488 .
- 5 -8 55=<95
. (
-54 ) 55
.2117 . -9 564555
) (Phoenix dactylifera L.
. 63-54: )1(6 . ( 5 )Phoenix dactylifera L.
-55
.2118. 5 5
-: .1988.
. . (Phoenix
. 71 -59 : )21(2 dactylifera )L.
5 644< 5 -56 . -
.
5 ; - .2119 .
5 .
5
- . 255 .
.
13- Abbas, M. F. ; A. M. Jassim
< - 564495
and Ibrahim, A. O .1995. Effect
of pollen endogenous hormones
on the fruit of the date palm
()Phoenix dactylifera L.
9:
2461 ) 16 04 ( : ) 2 ( 8
18- Bacha, M. A. A.; M.A. Aly; , (Phoenix dactyliferaL.) cv.
R.S. Al-Obeed and Abdul- Hillawi. Basrah J. Agric. Sci.,
Rahman, A. O .2000. 8:33-41.
Compatibility relationships in
14- Al-Khalifah, N.S.2006.
some date palm cultivars
Metaxenia : influence of pollen
(Phoenix dactylifera L.).J. King
on the maternal tissue of fruits of
Saud. Univ. Agric. Sci., 12 (2):
two cultivars of date palm
81-95.
(Phoenix dactyliferaL.) .
19- Ghnaim, H.D. and J. A. Al- Bangladesh J. Bot., 35(2): 151-
Muhtaseb .2006. Effect of pollen 161.
source on yield ,quality and
15 - Al-Muhtaseb, J.A. and H.D.
maturity of Mejhool date palm.
Ghnaim .2006a. Effect of pollen
Jordan J. Agric. Sci., 2(1): 8-15 .
source on yield, quality and
20- Hafez ,O.M.; M. A. Saleh; E. A. maturity of Barhi date palm .
M. Mostafa ; M. M. Naguib and Jordan J. Agric. Sci., 2(2) : 9- 14.
Ashour, N. E .2014. Effect of
16- Al-Obeed, R.S. and A.O. Abdul-
pollen grain sources on yield and
Rahman. 2002. Compatibility
fruit quality of Samany date
relationships within and between
palm. Int. J. Agric. Res., 9(3) :
ten date palm cultivars (Phoenix
164-168 .
dactylifera L.). I-Fruit set and
21- Iqbal, M.2005. Effect of various yield. J. Adv. Agri. Res., 7(4):
dactylifera males and pollination 809-820.
innovation in fruit setting and
17- Al-Obeed, R.S. and S.S.
yield of ( C.V .Dhakki. Ph.D
Soliman .2011. Effect of delaying
thesis , Faculty of Agriculture ,
pollination on bunch weight and
Gomal University , NWFP-
fruit quality of Barhy date palm
Pakistan) .
cultivar under Riyadh condition.
22- Iqbal, M.; A. Ghafoor; Jalal-ud- Am-Euras. J. Agric. & Environ.
Din and Munir, M .2008. Effect Sci., 10 (1): 65-69 .
of different date male pollinizer
9;
2461 ) 16 04 ( : ) 2 ( 8
phylogenetic relationships with on fruit characteristics and yield
Amhat cv. date palm (Phoenix index of date palm (Phoenix
dactylifera L.) in Egypt using dactylifera L.) (C.V. Zahidi and
RAPD markers. Int. J. Agric. Dhakki . Pakistan J. Agric. Res.,
Res., 9(7) : 331-343. 21(4) : 79-85.).
9<
2461 ) 16 04 ( : ) 2 ( 8
Rajwana ; B. A. Saleem ; M.
Amin and Ahmad, I .2011.
Influence of pollen source and
pollination frequency on fruit
drop, yield and quality of date
palm (Phoenix dactylifera L.) cv.
Dhakki . Pak. J. Bot., 43(2) :
831-839.
9=
2461 ) 16 04 ( : ) 2 ( 8
The study was carried outto study plant hormone contentof Pollen grains for nine
seedling strains of male date palm in addition to Ghannami Akhdarcultivar and
their Influence on Barhi and Sayer cultivars production in Al-Mahaweel date palm
station at province of Babil. Results was shown that the highest content of auxin
(IAA) was recorded in clone 9 and Ghannami Akhdar (5.359 and 5.486
micrograms / kg fresh weight respectively). Ghannami Akhdar also gave the
highest content of Kinetin (5.376 micrograms / kg). While, clone 2 and 5 gave the
highest content of Gibbereline (GA3) (10.875 and 10.699 micrograms/kg
respectively). The highest concentration of Abscisic acid (7.971 micrograms/kg
fresh weight) found in pollen of clone 3.The impact of pollen grains resource in
the qualities of productivity was recorded. Barhi and Sayer fruits hashigher fruit
set when pollinatedwith clone 6 pollen grain in the first season and clone 2 in the
second season.while, clone 3 and clone 1 in the first season and clone 5 in the
second season, recorded the highest fruit abscission percentage .Clone 2 and 5,
gave the highest rate in the maturity of Barhi and Sayer and fruits for both
seasons. Also, clones 7 and 8 and cultivar Ghannami Akhdarrecorded highest
total weight of the raceme and winning. Barhi cultivar was superior than Sayer in
:4
2461 ) 16 04 ( : ) 2 ( 8
traits studied (fruit set , bunch weight and total weight per palm) in the three
stages of maturity Khallal, Rutab and Tamer for both seasons.
:5