Basic laboratory methods in medical parasitology @) X yy World Health Organization GenevaReprinted 1997 WHO Library Cataloguing in Publication Data Basic laboratory methods in medical parasitology. 1. Parasitology — laboratory manuals ISBN 92 4 154810 4 (NLM Classification: WO 25) © Worls Health Organization 1991 Publications ofthe Warld Health Organization enjoy copyright protection in accordance withthe provisions of Protocol 2 of the Universal Copyright Convention. For rights of reproduction oF {translation of WHO publications. in part or in fol, application should be made to the Ofice of Publications, World Health Organization, Geneva, Switzerland, The World Health Organization welcomes such applications The designations employed and the presentation ofthe material i his publication do nt imoly the expression of any opinion whatsoever on the part of the Secretariat of the World Health Grganvzation concerning the legal status of any country. tertitoy. city or area or of authorities, Sr concerning the delimitation of is rontiers or boundaries. The mention of specific companies ar of eetain manufacturers’ products does not imply that they are endorsed or recommended by the World Health Organization in reterence to others ofa Similar nature ina are not mentioned, Errars ane omissions exceptes, he names of proprietary products are distinguished by inital capital leters TYPESET IN INDIA PRINTED IN ENGLAND (888914-Macmilan/Clays-7000 9711 1444-Clays-1000Contents Preface List of contributors Introduction Laboratory safety Section 1. Techniques of collection, preparation and examination of samples Care of the microscope Callbrating the microscope for measurement Faecal specimens Collection of faecal specimens Examination Macroscopic examination of stool Microscopic examination of wet mounts Identification of parasites ‘Supplementary techniques Concentration technique Permanent staining techniques Identification of parasites in stained smears Anal swabs for Cellophane faecal thick-smear for diagnosis of intestinal schistosomiasis (Kato-Katz technique) worm ‘Sending specimens to a reference laboratory Disposal of specimens Quality control for faecal examination Collection of specimens Preparation of reagents Performance of techniques Urine specimens Collection of urine for diagnosis of Schistosoma fection Examination of urine Sedimentation method for 24-hour terminal urine collection Syringe filtration method Identification vii viti 10 10 10 13 15 16 7 24 24 25 28 29 30 30 31 33 33 33 33 34 36