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sasoyyared uy ua any UH, BUEN pu ‘saudi “sheayy asacope suet. aypovadans aren agoyadeS ABU ‘suo sau NUE URURDAD He W080 ‘gute uote EU ay PLE Puen "ie paLodEy | jajonu jeujuasaigns ue yosoIw :aoeds peat Yous 4o seunjeay fay ‘ajeajonue ‘paqiod ‘ayeajnue “sapuays “Guay ‘parade, Oss OOL-se (022) 862-0ve (862) zie-cu2 (ze) ose-22t (092) 98-rre (rea) vee-e0a (182) 006-022 (eat) e02-91 (982) ose-12 2 _ juasqy _ juasay qwasay wasaig qwasald qwasald qwasald ; — _ _ ‘upouady oiponady jeusnig, feusMOON {eUINYOON [PUINOON =| bis ws pooig poo Pooja poo pooig : ssanssi, enrjourtuos = sada9p pue sanssiy ‘senssi} Ss snoaueynoqgng swag snoaueynogns, snoauenaqng §—weisks oyeydwe] §=— weiss oneyduky weiss aypeudusky weyaey yinpy 3 unyrays sopay uosueyy 2 ‘Sal OIG ‘saaydouy ‘sajaydouy wingnuts SApIOIIN) S@pIOOyND. SAIC sdoshiyg ‘Buosuey ‘sapay ‘xeng 2 saypypeg seb pw Gua :sobpiu Sung saBpau Sung say pruege, :S0yNbsOW ‘snDsO} si0}0A 2/8 Pavey ynog wauyuoogns cls uy rene, Poneuy Yinog ue jenuan, eouly ueipyy ns S| wwa'euy ue 1sam ue eo “veaqqueg ——_erivag pue sam uejseuopuy sy see-4NNOS pe sudo, wali = eC sajnayor eosan0}da.s suegsiad spsezz0 oun, sheyeus yossueg 3 easa9049u0 ‘eyjavosuey eyjavosuew eyeuosuey 20] 807 eying ‘erong euratagaay, saisads 3 “ i @ saysound poUDpY UoUINY WoUIUIOD Jo $>145}40)90104) *| 2]qD] Bench Aids for the diagnosis of filarial infections {© Worl Heath Organization 1987 Introduction Several species of filarial worms infect humans in the tropical and subtropical regions of the world (Table 1, overteat), The adult worms inhabit various tissues and organs of the body and are inaccessible for identification. Consequently, diagnosis of filarial infections depends primarily on the identification of the larval stage of the parasite (microfilaria). Most species of microfilaria circulate in peripheral blood; however, someare foundin the skin, {-microscopic level and with the aid of a variety of stains, a microflaria appears itive organism, serpentine in shape and fed withthe nuclei of many celts. Figure 1 ig @ diagram of a typical microfilaria, In many, but not all, species, the body may be enveloped in a memibrane called a sheath (sh). Where a sheath is present it may extend a short or long distance beyond either extremity of the microflaria. In some species, depending on the stain used, the sheath displays a characteristic staining quality which aids in species identification, The nuclei ofthe cells that fil the body are usually darkly stained and may be crowded together or dispersed. The anterior extremity is typically ‘devoid of nuclei and is called the cephalic or head space (hs); it may be short or fang, ‘Along the body of the microfilaria there are additional spaces and cells that serve as anatomical landmarks. These include the nerve ring (nr), excretory pore (ep). excretory ‘call (ee), and anal pore (ap). In some species, an amorphous mass called the innerbody (ib) and four small cells called the rectal cells (Ret, R-2, R-3, R-4) can be seen, usually with the aid of special stains. These structures and their positions are sometmes useful for species identification, The shape of the tall and the presence or absence and

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