BI TP MN SC K

Nhm hc vin: Phm Vn Quang

Nguyn Th Hng

Nguyn Th Mai

Bi tp 1: nh lng nguyn liu Cefuroxim Natri

- Dung dch chun ni gc: Dung dch Orcinol c nng 1,5 mg/ml trong nc

- Dung dch th: Cn chnh xc khong 25 mg Cefuroxim Natri vo bnh nh mc 25 ml, ha

tan v pha long va 25,0 ml bng nc. Ht chnh xc 5 ml dung dch ny v 20 ml dung
dch chun ni gc vo bnh nh mc 100,0 ml, pha long va bng nc

- Dung dch chun: Cn chnh xc khong 25 mg Cefuroxim Natri vo bnh nh mc 25 ml,

ha tan v pha long va 25,0 ml bng nc. Ht chnh xc 5 ml dung dch ny v 20 ml
dung dch chun ni gc vo bnh nh mc 100,0 ml, pha long va bng nc

Tin hnh chy sc k

Kt qu: Mu chun:

Mu chun: Chun cefuroxim Natri c cha 923 g/mg Cefuroxim nguyn trng(Mcefuroxim
Natri = 446; MCefuroxim= 424)

Khi lng cn: 25,21 mg

Din tch pic
TT Din tch Pic Cefuroxin Natri Din tch pc Orcinol
1 412,59 200,89
2 410,43 200,79
3 410,33 200,54
4 410,92 201,06
5 410,17 201,12
6 412,59 200,89
Mu th:

TT Khi lng th (mg) Din tch Pic Cefuroxin Natri Din tch pc Orcinol
1 25,49 412,23 201,55
2 24,33 390,36 200,25
3 24,79 400,12 201,72
4 26,13 422,73 201,17
5 25,35 411,33 201,8
6 25,44 409,39 201,53
Xc nh hm lng cefuroxim c trong cefuroxim Natri, tnh theo nguyn trng

Bi tp 2: nh lng nguyn liu Loratadin

- Dung dch th: Cn chnh xc khong 20mg nguyn liu Loratadin vo bnh nh mc 50 ml,
ha tan v pha long va 50,0 ml bng dung mi pha mu thch hp.
- Dung dch chun: Cn chnh xc khong 20mg Loratadin chun vo bnh nh mc 50 ml, ha
tan v pha long va 50,0 ml bng dung mi pha mu thch hp

Tin hnh chy sc k

Kt qu: Mu chun:

Mu chun: Chun Loratadin c hm lng 99,90 %, tnh theo nguyn trng

Khi lng cn: 18,96 mg

TT Din tch Pic Loratadin
1 15953,5
2 15961,2
3 15967,1
4 15973,2
5 16009,1
6 16005,3
Mu th:

TT Khi lng th (mg) Din tch Pic Loratadin

1 21,60 17915,4
2 20,29 16907,3
3 21,20 17760,5
4 19,97 16664,6
5 21,49 17838,3
6 19,61 16308,5
Xc nh hm lng Loratadin c trong nguyn liu Loratadin

Bi 3:

Xc nh tp cht lin quan ca Cefpodoxim proxetil theo phng php chun ha din tch. Kt
qu nh sau:

Tn cht Thi gian lu (pht) Din tch pic

Tp cht 1 31,92 169,10
Cefpodoxim proxetil S-epimer 33,39 9139,98
Cefpodoxim proxetil R-Epimer 37,23 9286,44
Tp cht 2 52,93 39,82
Tp cht 3 72,73 72,64
Tp cht 4 74,79 98,83
Tp cht 5 76,14 39,30

Xc nh hm lng phn trm tng tp cht Cefpodoxim proxetil

p n:

Cu 1:

- Nng Cefuroxim trong dung dch chun:

= =

- Nng cefuroxim trong dung dch th

= =

- T s din tch pic ca Cefuroxim Natri v Orcinol

+ Trong dung dch chun:

Rc l gi tr trung bnh ca 6 ln tim dung dch chun

+ Trong dung dch th:

- Ta c:

Nn :

= = =

Suy ra:

=

Kt qu: Tnh Rc

TT Din tch Pic Din tch pc Orcinol T s din tch

Cefuroxin Natri
1 412,59 200,89 2,05381
2 410,43 200,79 2,04408
3 410,33 200,54 2,04613
4 410,92 201,06 2,04377
5 410,17 201,12 2,03943
6 412,59 200,89 2,05381
Trung bnh 2,04684

Hm lng Cefuroxim

Khi lng cn chun: 25,21 mg; hm lng chun: 923 g/mg Cefuroxim ( nguyn trng)
TT Khi lng th Din tch Pic Din tch pc T s din Hm lng
(mg) Cefuroxin Natri Orcinol tch
1 25,49 412,23 201,55 2,0453 912,18
2 24,33 390,36 200,25 1,9494 910,84
3 24,79 400,12 201,72 1,9835 909,61
4 26,13 422,73 201,17 2,1014 914,22
5 25,35 411,33 201,8 2,0383 914,08
6 25,44 409,39 201,53 2,0314 907,76
Trung bnh 911,45

Bi 2:

- Nng Loratadin trong dung dch chun:

= =

- Nng Loratadin trong dung dch th

= =

- Ta c:

Trong : Sc l din tch pic trung bnh ca 6 ln tim dung dch chun

Nn :

= = =

Suy ra:

=

Kt qu: Tnh Sc

TT Din tch Pic Loratadin

1 15953,5
2 15961,2
3 15967,1
4 15973,2
5 16009,1
6 16005,3
Trung bnh 15978,23

Hm lng Cefuroxim

Khi lng chun: 18,96 mg; hm lng 99,90 %, tnh theo nguyn trng
TT Khi lng th (mg) Din tch Pic Loratadin Hm lng Loratadin (NT)
1 21,60 17915,4 98,32
2 20,29 16907,3 98,78
3 21,20 17760,5 99,31
4 19,97 16664,6 98,92
5 21,49 17838,3 98,40
6 19,61 16308,5 98,59
Trung bnh

Bi 3:

Cng thc tnh hm lng phn trm tp cht

% =

Kt qu:

Tn cht Thi gian lu (pht) Din tch pic % tp

Tp cht 1 31,92 169,10 0,90
Cefpodoxim proxetil S- 33,39 9139,98
epimer
Cefpodoxim proxetil R- 37,23 9286,44
Epimer
Tp cht 2 52,93 39,82 0,21
Tp cht 3 72,73 72,64 0,39
Tp cht 4 74,79 98,83 0,52
Tp cht 5 76,14 39,30 0,21
Tng 18846,11 2,23