MCB123 NAME KEY Student ID # QUIZ 2 NOTE - THIS IS TWO SIDED! 1) (2 pts.) Briefly list four assumptions that are made when deriving the classical unireactant Michaelis-Menten equation under rapid equilibrium conditions. (eJe= (e+ (es) (E)4C8) ae eare VEN ¢ Pj=0 EMMIS CONSTANT s) & (5) > (eke c) qa) v= ko alg 2) (2pts.) Given the uni uni reaction: k. E+S == Es E+P Ifwe assume that k; = 6 x 10° M" x seo”; k = 3x 10° sec’; and kz = 9 x 10? sec Calculate values for K, and Kn (give correct units!). ts = ew ku = k-) +Re Ri Ri 3x Io" 3x1ot + 1 XI0% Sxi08 6x10 Ke SOnmM Kn= 2OOuIA sxio FM axon A 3) (4 pt.) Adding a concentration of [S] equal to 6 Km to an enzyme reaction will give BS — 6 % of Vnex ~ 6.4) (3 pts.) Product inhibition can be depicted in a similar way to the framework describing a competitive inhibitor: he E+ S == ES —> ep % EP ‘The equilibrium dissociation constant Ks = 4 uM. The equilibrium dissociation constant Kp = 2 uM. How much more [S] is needed to attain 0.5 Vex when [P] = 4 uM compared to [P] = 0 uM? ye eke fee Or es¥ev) Vmax ies ag, oe 2 a O vos — Tne ao. “Tmar 6 (42 HO KiB) = get(ie ES) = = (vee = 3K —— 5) (2 pts.) Using the reaction scheme below, calculate the dissociation constant aKs and the cooperativity factor pe ity a: 30 E+A SS ca + +B +B (is) (40) =(g0)(&ks) 's «| | | [- * —B+A = EAB ak, Go When: Ka = 80 nM Ke = 15M aK, = 40 nM akes 150M ge O'S