Take home assignment, Micro 443 Name

Keep answers short; complete sentences not required. Due the Monday that follows the first day.

1. Have you read the CDC information regarding the handling BSL-2 organisms? (hint: give an
honest answer, recognizing that "no" is not acceptable)

2. In the lab:

a. Where is the closest fire extinguisher?

b. Where is the closest eyewash?

c. Where is the closest shower?

d. Where is the laboratory safety information kept?

e. What is the purpose of the sand/alcohol bottles?

f. What is the danger of an aerosol?

g. What is the approximate ID50 of Shigella?

h. Why are there separate discard bins for glass and disposable plastic?

3. With respect to the Bunsen burner:

a. When should you use the pilot light rather than full flame?

b. Why should you disconnect the hose at the end of the lab period?

4. With respect to the microscopes:

a. Why do you wipe the oil off the 100X lens at the end of each lab period?

b. Why shouldn't the 40X lens be passed through the oil?

c. How should the microscope be stored? (describe the placement of the lenses and
stage)