BRINE shrimp

OUTLINE
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toxicodynamics
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Two major factors in exposure situation
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Characteristics of exposure
Route of
exposure

Duration and
frequency of
exposure
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Two types of dose response relationship

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Two types of dose response relationship

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Brine shrimp lethality assay
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Advantages
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Brine shrimp lethality assay

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Probit analysis
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Probit analysis in BSLA
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Probit analysis in BSLA
Procedure
Procedure: sample prep’n
Procedure: assay
Procedure: assay
Procedure: assay
Procedure: assay
Procedure: assay
Procedure: Probit Analysis
Finney’s Table
Procedure: Probit Analysis
results
 Alstonia scholaris
log concentration Trial 1 Trial 2 Trial 3 Average SD % mortality Probits
10 mcg/mL 1 0 3 3 3.00 0.00 30 4.48
100 mcg/mL 2 4 3 3 3.33 0.58 33 4.56
1000 mcg/mL 3 6 5 3 5.50 0.71 55 5.13

Chrysophyllum cainito
10 mcg/mL 1 2 1 1 1.33 0.58 13 3.87
100 mcg/mL 2 1 1 1 1.00 0.00 10 3.72
1000 mcg/mL 3 1 5 4 4.50 0.71 45 4.87

Mangifera indica
10 mcg/mL 1 4 4 1 4.00 0.00 40 4.75
100 mcg/mL 2 4 4 4 4.00 0.00 40 4.75
1000 mcg/mL 3 3 5 4 4.00 1.00 40 4.75
discussion
 discussion

Changes in procedure

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 discussion
 dilution of the samples instead of As in the
original
 well plate setup more difficult to manipulate
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discussion
Theoretical Actual
discussion
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 Conclusion
recommendation
 Conclusion
recommendation

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THANK YOU!
REFERENCES