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    Carte de bacteriologie medicala

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    28 views239 pages

    Bacteriologie Medicala Olga Mihaela Dorobat

    Uploaded by

    Elena Ionescu
    Carte de bacteriologie medicala

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    of 239
    OLGA ~ MIHAELA DOROBAT BACTERIOLOGIE MEDICALA EDITURA UNIVERSITATH “TITU MAIORESCU” BUCURESTI 2006 OLGA~ MIHAELA DOROBAT BACTERIOLOGIE MEDICALA EDITURA UNIVERSITATI “TITU MAIORESCU” ISBN 973-7674-00-6 ean: aura PRINTECH (S.C. ANDOR TIPO S.R.L.) ‘St TUNARI nr.11, sector 2, BUCURESTI ‘TelRax : 211.37.12 Lumea bacterillor Bacteriologia medial ste ea oie studial baseior cae pot ein ol nese lao. Bactrologia ee 0 parte» Mirobisloiet slit de Virusoogi, Miclogte 3 Parztogi. Balle congas se propaga mod epider, Muth vem canes san pus inter ssopra at acs contol In pron renaptr Jette Freastor fn hears sn De conalane cntagioss mobi et suraice” in 1546 emite een umor parce Vk ae pot contami orl care pt eines uror bol ranamie Evista baer a fst eeaoscls per prima da de Aavoie ‘von Letuvenoek (632-196), rin loses tm microseop sini eu imiie x 270 Leeavenoek observé mist "animalele vile eu oil Ihe, ope pe cre rele neo sro travis a Bish Rayal Soe in 7Tsepembre IGT. Desenle sesso miei animale” sven fs deste, bustonay auspices ce corespnde morfolog debt baer, Tots! stuilbcterior ncepe de abi a seal XIX prin coma ‘4 dout mar personlit: Loue Par Rober Koch, Lowis Pasar (1922-1895), profesor de chimie Ia Universita din LileFanj a demonstar et frmanaile aloo, atic lal st ecemiate de misroormisme speifie, ote ce permis elbirare tor Imoiode de culivare a baci § a anor prosrdee de stlizare Ap fn riders natura lnfecioas a aor balls vierilor de malse pcan gi 9 ‘tor boll ale emul gale aninalelor. Louie Pastor a descopeit apt era pina cad vaceiaren ew microorganism atenuate su inactivate Pasteur demonsreass fal ed Paoanee cu meal strate last a SC [20°C rani totenona stile, des avea dopa de va. Atel prin tulle Iul Pasar fu inbslat convene pivnd fora general ‘spontane, conform ela Sa creat mulls rene ok onele vue se mse spontan da materi pate Robert Koch (183:1910, medic german, pose fh considera, Intemelctoralbaeterologiet modern dt error sal rin sae pone 4a punt, tehncie bacterologlee (ulizea ula pent examines imiroseopie, invodueeres medilor de curs solide in pracien eli escoperit bail tereals demu gba ctrl). Robert Koch Heya esr roa pation. Destomenea deste wblons! used deaiceracle Ti Henle asups speci infer mirobiene, Roch fvnulecl cele trehpostlate caret oars mumele, refetare l ‘inn btn uel inet Toul Patew si Robe Koch ao asigurat_ progress rapid al cobisog ot pio propiledescopert et in formare de elev care ‘Prema andl lr pon perfomance Dsweromdn Vitor Babey sf loan Caxtacoino a contibit Ia erolins mobile "Vicor Babes (1850-1926, medic astome-paolg, suns a eres «de nicubilogiepesiné dela sil hstapuloic a oceans. De umele stu sin legteo sre de coe asopasrctri basen sulle oetecomatce ale babii dire (abeyErs), gralele FRobectilr, desires earscllr cin creel animalelor moar de fare (Bebe Negr, descoperea a dau nol speci de protozoa dia smut obesela ea si supa unr boi inejons, ‘Vicor Babes sxe contor al prlmufet trata de bactrilgie smelt din lune’ "Les tater ter al dans Tetlog, anatomic et Thiasiogicpuolosiguey des malades infects" par A. V. Col et V. Bibs, el eli Alean, 1885, Pais. A Tint prim nti de cxoai Pil dn Romina iat eae pont mumele fim npn prinele Tartare de gle bacteriologi de la noidin ar ean Cantacuzin (1963-195, mee Comat a TsialPastear din Pars ta labor Ii Tia Mecinko, unde a aera timp de nous ath Continuat eel ssypra imum Ta ncvererate, a aprofidst nee ‘tyes ale raortll miroorganim: gud In ptogens ler eatin, ‘Mheeloe! qf exanemate.Toan Canaetzno inrodas in rs ‘Sroses vccnareaanslerih BCG A reaps ree o devia foals fominewcd mode de miccbiologi ial ia Labororal de Mees Experimental pe eae Fe conde In cara Fcufi de Mediind dn [ay po in nit ere poaré mune ont in 1921). Cnooyerea_ tacrlor ene necesurd fined bolle infetose conn tn aecoll XXL fo cata poate morbiiatss! martaliste fn imeaga fone, © setie deface precum medietle climate, {snoptie,ttnlogce, deaokare small ome conta It mergna fi reemerene ur bl neji In pus baci ‘el lene pgs, epi de deen Wa pl recs {sl lel pepe sbcteenvode oenpesth ps ee vise c ata sa dpt modifi pete ‘actiolgsete eontin acinar pn eicaces anor ‘et ies. i dtc msi cave cl ee, pit Sui de Nlope’ molecular uate rie ‘chines uorpecitatenpe TNE MME A Teenie Flint i ea classe out tear: vege sina pa in 136 cn Ht pepe Pip nt ne ce seca tee hm da in 1937 Chto propane a eon vnc ogra Pri in Euewia (le, pocnre sng) Pct el ge aban beads pence mn acta ae) ‘arecsDits Pocate st somite eft Ease acca Cet Arata cade tpl ie de ogee one maapn, lee emer rotten el pl ser abe ede a lev rocariote. Restul de procariate, in afar de Archacbacteris cia pei Rs ev Arent Cjebcea Bacteria oinime vi de dines ree ase dis sings cell dep prea are pia dvichne des Segre ep ocr cae individ men ele seu EERE de 9’ Nom Rear ese deste de teil prin dnensianea malic prin parsimal acl. ge “obs 1 Casters le celular procarit i eueaine 1 Morfologia si structura colulei bacteriene Dimeoslunilebactior se siueai sub linia vz eu och lier sf de aseen obserarea lor se poste realize mumai x sherl eroseopa (© sic de elements ale celle tacterine (limeasinil, fom lupus, mobili, inclaile, spr, capa) pot fi puse Ia evident ricrscopal optic. Pct siren souctlor mal fn este neces enkes mctosopuli elecroni. ‘Stal morologel si a seucurlor normale ale tacteritoe ae ‘fecweazt pe cla tnere nv, devote pe mei fives end ‘ptme de i temperatr i osolriat, [enassire [Forma bacteviee | Tame | canes | _ Cre ene | ie ine ie rreninna | ase ro sie sackol —| ae roa ‘roncnaae ie ‘5 ml (singles | (mies Awe | pein, | spartmoe| aise tere ico = Sis Tiopisma ote | Sa pee | ‘ioe [708 os | erste ular | pea ba IS (onoe ‘simale Peolngcy tee iS a cone septa) Misimes ETON S my a ce ee cella Fa sso eres aor pret rotund (ap Tococaa fami oval (Srepoeoensprogenes) CCoci__janceol (Sepocosas pon) ifm! (Nese ganoraeae) acter ub forms de astoane cep sur cre Coe po eos de abc! dp oma eepetlor Basi |" route (Bac sil) -scufe Fuobaterim ftforme) “acct (Cnpwbactran phere) late dep (Baccus) Coeobosiy facet ur alunge etemediare ee cod Waal Pasteurla, Bordellaperturs, Brucella) | ‘Viboni tater carte sab frm de vrgl sau sin br letra) Spirits ae aa Tome une apy esp coral Basa spirochetes en sie igi (Sr), at ln speochete copa et lesb Prone, Borel, eps) tino pacer sab frm de lente ng, annie Ca nee, care we agentes foe base fn cult veh 1.4, Forma bactorilor ops forms bcterileptfcasiea at cx coon Crocs tt —_ —— we 9) Spit MCS Spesdite VV satanic mah igea L1Forma basrilor Vora le famei beterilor s© consath Ho seseai conti x 5) petra imeriaes oe 42, Dimensiunes hacterilor imensiaes bacteria e miso amiconn=10-m). nme obo caries dimen! ie 0-1Oh gros / 3 sym ngime cu vv in fue de pei ema bce, mafia de eur iia catar a Scar mal vec bate av dimensini ai mari dee cele anette specie, Varin ale denial tacterior = cons 5 in {Tite pert clnce ca amare a nerve! mecaismeloe €eaptare a ‘egnisol ged eventuaeloruatamentecu abit. ‘ 1.3. Gruparea bacteriior ‘Moddl de grupae a bts, uncon erate pecs, gen, fui bactcrene est in fuetie de orcntaea tn pia a iferitls plu sceesivedediviziune se tendaja cell fice dea rimine unite ‘Cand planurile succesive de diviziune sunt paralele se pot forma perechi numnite diplocoei(Siepacoecis petmoniae, Neder ‘meningitis i plobci (Aldea sp.) a ln de angi varie streptooet (Sireprocaces sp.) sa septal (Baill amthral) Sa ‘me de V;M, paisa forme care se produc printea mia de alunestes ‘culo ice ina fi do cea (Corynebacterium dlpterie) ‘Dacddivicunes are oc in doud planer! perendiclare reals 6 ‘grupare constitu din 4 celle tae (Airococcu etragens) ar Gack tiviionen se face in rei planus perpendicular real un ub Je este (Sarcinay: plant diferte au ea reruat ages) neregulate cloching (Suapococeussp), Coc: Diplo ow Preumeroe Diplo ap Meningoeoe La Seeese sip Gut 8B Terade ows —— Corte & sattoe Bact Dy ND MVK ~ —— = Figura 12 Gruparea bacerilor 14, Structura coluleibacterione rl ingle det xl scat, acti 0 egare completa oun xs incr ica cont cle ‘sitcom mone destucnoSgaoni-oue lopisna mebana Choplsoatet pete eka Ce uct cla Rak cap, sll pi elopr 4.44 Nucleut en on moi in ie emt “in oneel nucle ene stan partes central aclu, ind gst ceo er mngmen ems ees son Si ea socom ican Soe uct, fh cold ADNl est impact sb fora nor base supe Inigrate cae sun nie Ia bask fr menperen slo fonmh capace scl se pared au nol ARN spo wh -Autoreplcaacromazomulbaciesan este dip semconservt, a fecare moleels ADN nou fora a polnuclestc prove din la AADN parental un atest nou sia, Funel Nekt out ira gests neces anor rgmiznil siete nina rl baton, 142 Cltoplasma opis, sin tre nuclea sf membrana ciples, exe un sisem cladal iu din apreimatv 80% apn ee ego: ean mare de molecule ergnice mich loni ancrganil core, ARNG CARN "osama, ARN (ARN mesage, ARNI (ARN de fe) insta dese, ‘acol i unele ace plrmide(ADN extaromoreria ‘Lipsit de organi celular ctopasma putea beni are wn asec. fn omogen granular soi mtu mare de boson, "Ribosomil— in numir de 20 000-4000, reper 40% din rows elle! ust, ind principle clement ale copasmel. Au emt de sediment de 70S: In anurite condi ribose por deste do sobuniti de om nerepun cu consane de sedeniare de 308 305 La siveal ebosonior se relizszt basiners proteinel, una dese ra! ‘impor eape pena vi cell. lformaia penta slgres 9 ranjars tmincaciaor to adel jul plete et conde comercmul bectran. De ls ADN informaia se eanmite lenis ribocoor nde ‘inosi neces frm aul poietic sta de ARN Teturile sant struct ie de dimen’ former ofc 1 conde de- med, unear iconjurte de 9° membandproted shai ‘Dap compari himis inl po fl lasifat ate ‘+ inclu polgacsiice (gicoge)~ spar sab forma une depoie dense Sau pean a cloplaena ma alsin nl exces dectbon te mea 6u Himtares azo, fsfoull gt sul sa ciod pital ese re stat + Incr de pottetahidroxibtiat (PHB) -compuse da PHB, poco, le de ie esr, pot st ere mai mult de 80% dn gree ° uta colle dct batrile uncuivte a medi x carbon neces ned ener |, eat de polisfitsnorgaicl, nome grrue de volng, descise pina da la Spon volar sau eompsclt Babe nest ln see apar sv Toma wer Soporte sfcies eae os colored i ce colori alba bas dete, abst de tid, de Se demi de grant metacromatce ae atractrt paracrine se pot obser ineosh specie + ete din gral Bails # Closridum, legit de proc spor ‘rata de sal - pearls boc slficase eescte medi ‘eset pci th gener materile de ez san srse de send ese J sctvistes metabolic cell cmt ile ok aven in ctoplaanl vcolegaruse imate de 0 exit nian, err fet es ne bine cunosct 143 lnvoligullecolulare ‘ose stile cre scoped esa procarotsunt dnumite fpr cig celle. Soc ranzarea ivelfprior cellar de re eetomrparve ¥ Oreaegatve in cor Gram! ene bacterile care se colored viet sunt seotie 1 Gromposive fr cele care au eulosren rosie suo denunite nse. Th baerile Gram-portie suc et rel snp, onside ei: menbran copasmalc, un sa rs depeptoglicn un Seat rl dum cpl Te bcerle Gram-negative sroctra ete mai complet: mebeana cyst oar demir de membrane wen an sigur sat Sitpscelcan, membram exter gi exter cps. Tne membeena cece a il pease ‘Gansta Gram: violet de geniand -Imin,solje lugel - 2mia, aleoot - 0 ee Figura 1.3 Inelzritebacterior Gram postive Grom negaive 143.1 Mombranacltoplasmatica margineste citoplasma separind-o de percicle celular. Este vizi Sie microscopul electronic. : oe ‘oul se ndeprteact de pete detricractcveneni 7 sues zonal penta bate Gra pontine roe Jizozimutui si EDTA pentru Gi Pro a siopln cap \EDTA pet Grm-neeatve. Poli epi es form sleet in met hipeine (nde debaccisdi ca ‘arin medii hipotone se lizeaza usor, a iegie ghia Clonaoniet mers igi sunt mess Functii ‘ + Memtana.itplasnict c r ste semipemeabill,avind ol de baie emotes. Treseea pin membrana coplasmatil nae we 4"

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