METHODOLOGY
In the beginning the researchers were first asked to prepare a BCA solution this is prepared by mixing
BCA reagent A with BCA reagent B (50:1).
Determination of 𝜆max for
each protein assay
500𝜇𝑔/mL was used for
standard BSA solution. BCA
method was performed.
The absorbances of treated
standard BSA solution from
300nm to 900nm was read.
For BCA the readings are for
every 25nm increments.
The absorbance against the
wavelength was plotted. After
that, the 𝜆max for each
protein were determined.
Determination of dynamic
standard curve and limit of
detectability for each
Protein Assays
protein assay.
The reagent blank, standards
A standard BSA solutions by
and unknowns should be
two folding dilution of
measured in the same
500𝜇𝑔/mL BSA was prepared.
experiment.
The 𝜆max obtained from the
procedure A was used to
perform BCA method.
The absorbance against the
concentration was plotted.
After that, the dynamic
standard curve and limit
detectability for each protein
assay were determined.