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    33 views28 pages

    Miller 6 PDF

    Uploaded by

    Jonathon Hart

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    of 28
    RNA Processing, Modification and Splicing {RNA 5' and 3° processing Base Modification Intron Removal rRNA Separation Processing Base modification Intron Removal mRNA 5'- Capping 3' - poly A addition Intron Removal Organellar RNAs Mitochondri tRNA mRNA Chloroplasts rRNA mRNA tRNA tRNA Introns Group I Introns Spliceosomal Introns Group I Intron Group I Introns Group II Introns Group I Introns Group II Introns Group I Introns Group II Introns Group II Introns 30 . aL Differences Between Bacterial and Animal mRNAs Bacterlal mRNA Animal mRNA Transcription unit Polycistronic Monocistranic Initiation (6" end) RNA polymerase binds DNA RNA polymerase binds DNA Elongation “40 nucs/sec “40 nues/sec S' end Triphosphate Methylated cap 3’ end generation Termination releases mRNA Cleavage releases mRNA S'end Last base of mRNA Poly(A) Transport Not necessary (Necessary Translation “45 nues/sec “20 nucs/sec Half life 4 hours Ribosomes/mRNA >20 >10 Bacterial a) 7 AUG uua + -35-10% sup p. AVG UuA i 7 PPP’ S5 A mal AG wa TATA 22 Aug ou 46 ou jus Goad [etme eee aa ey [5 esromg | RNA Splicing [preyed AUS dua A G ppp Pe ay, 83. Start of transcription Intron 1 Intron 2 Gene: Exon 1 Exon 2 Exon 3 Transcription eee — 1 Intron 2 Primary transcript: |: i} ee— Exon 1 Exon 2 Exon 3 Splicing Exon1 Exon 2 Exon3 | Mature transcript: &3 Splicing: Splice junction mutant: 3 cryptic splice sites: Cryptic site 1: AAG|IGTGAAC Cryptic site 2: GTG|GTGAGG Cryptic site 3: AAGIGTTACA Wild-type: CAG|GTTGGT S-QAGIGTQAGT +: AG|GTTGG Wild-type: [Exon Exon | —_____ TEx 54 Exon 1 Exon 2 gu—lntron 42 OH Step 1 one CS 3 MM | Aa NO” Step 2 Zz | . cm | | Spliced exons Intron | | GUG —U,Apuc——au'® OH t UCGp %6 Spliceo some Small ribonucleoproteins (a) Human small RNPs U6 pre-mRNA splicing 4x105 ut mpppG none Abundance — RNA Common | .NP Function (opies/cell) polymerase Cap antigen(s) * | {ucleoplasm . | Ul pre-mRNA splicing 1x 106 fie m GpppA Sm | u2 pre-mRNA splicing 5x105 1 m3GpppA = Sm | U4 pre-mRNA splicing 2x105 tt m,GpppA Sm | US pre-mRNA splicing 2x105 1 m,GpppA = Sm | | | & ' eeeee, 2°°e Ssu008 o oovabeoocus” 4 siptipsisecs S 88 o yorACUAUounnccn caps Py WENT. Une 4 ata cn : Prema a D> oa ena : a At (62) Pps I © Ca wre ~ : a CG. A22(C1) ‘Pm22 * — a ->— Ligstea product mane A2-3 (C2) 89 go (a) Male: no.Sxt UAG I tra pre-mRNA Oo “® | UAG i ona | rem ME complete tra protein b) Female: + Sx! (b) Female: + Sx! © 7 [om mRNA tra pre-mRNA ® [tase Complete tra protein awww yaaguwww ova dmUW I{(t

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