Isolation and Structure Determination of Two

Microcystins and Sequence Comparison of the McyABC

Adenylation Domains in Planktothrix species

Supporting Information

Guntram Christiansen,† Wesley Y. Yoshida, ‡ Judith F. Blom, § Cyril Portmann,⊥ Karl

Gademann,*, ⊥ Thomas Hemscheidt, ‡ Rainer Kurmayer*,†

Austrian Academy of Sciences, Institute for Limnology, Mondseestraße 9, A-5310 Mondsee, Austria

University of Hawaii at Manoa, Department of Chemistry, 2545 McCarthy Hall, Honolulu, HI 96822

Limnological Station, Institute of Plant Biology, University of Zürich, CH-8802 Kilchberg, Switzerland

and

Chemical Synthesis Laboratory, SB-ISIC-LSYNC, Swiss Federal Institute of Technology (EPFL), CH-

1015 Lausanne, Switzerland

*To whom correspondence should be addressed. Tel: ++41 21 6939315. Fax ++41 21 693 97 00.

E-mail: karl.gademann@epfl.ch (K.G.). Tel: ++43 6232 3125 32. Fax: ++43 6232 3578. E-mail:

rainer.kurmayer@oeaw.ac.at (R.K.).

†
Austrian Academy of Sciences.
‡
University of Hawaii at Manoa.
§
University of Zürich.
⊥
Swiss Federal Institute of Technology.

S1
 1
Figure S-01: H NMR Spectrum of 1 in CD3OH (600MHz)

CO2H O
H
N
HN NH
CH3 H3C O CH3
O O CH3 O
NH HN
H H H
CH3 CH3 N N
O
O CO2H O

1 OH
HO

S2
 1
Figure S-02: H NMR Spectrum of 2 in CD3OH (600MHz)

CO2H O
H
N
HN NH
CH3 H3C O CH3
O O CH3 O
NH HN
H H H
CH3 CH3 N N
O
O CO2H O

OH
2

OH

S3