1.

Column loaded with silica/column medium

2. Eluting solvent added to compact silica layer and to remove air bubbles
3. Purple mixture as a thin layer is added to top of silica layer
4. Eluting solvent added and eluted (purple layer separates into a red and blue
layer)
5. Eluting solvent added and eluted (red and blue layers separate further)
6. Red layer collected (the faster moving layer)
7. Blue layer collected (the slower moving layer)
8. No more compounds are eluted, process ended