400 mm Lpophrys pholis OT 300,000 fi Chelon brous OT 450 TS Parocemots ius OF (mm Octopus vulgaris RT 250,000 — 2 | Sarpa salpa (< 10.em) DT z 2 300 ‘mmm Sarpa salpa (> 20cm) DT = = = ~EFSA opinion 20000 E 0 2 Ostreopsis of. ovata 000 3 2 2 g 200 150,000 & 5 i 2 a0 3 2 100,000, & E 10 2 50,000 & Figure 2. Palytoxin concentration determined by the haemolytic test in the different tissue ‘components of the marine organisms harvested in Rochambeau from week 27 to week 36. The dashed line represents the EFSA threshold of 30 wg PLTX + OST-D-kg'!. The blue curve represents the abundance of Ostreopsis epiphytic cells over the same period.Table 3. Moan palytoxin (PLTX)-group toxin concentrations (hg kg) etemind by th haemolytic test inthe whole flesh (WE) of Fishery sodbts harvested in Rochambea from week 27 to week 36 Specs Common Name ‘ssw Analysed VLC 1 Faw Tops pals WF 938303 We ipods agar Wok 22s m Simpade melo WF 0% 12 oma om Simp nce wr 6% 12 om12 om Simpladas rosa Wo 6 am 12 om om Gotise Wool te Keouana uw Srid undesquamis Wo oe 12 0212 0212 om ‘aren helene WF 4 100% 12 0m12 0012 om Cons jus We 7 Be Ne oo12 2428 om Phalssoma pave WF 2 lot 12 om12 oo12 om Smphoda clan Wr 0% 12 om12 oo12 om ‘eran seriba Wr 5 100% 12 0012 0012 om Sophodas oat We 2 tom 12 om12 0012 om Malas mats Wey am 13 om12 091s om bada meanara WF s 10% 12 0012 0012 om Sorposaipajwvenies= 10cm) WF 633% 198 0.1 O12 TLATES 39% ‘Supa sap ads > 20) WF 3% oe 16 OZ 0919 oom Seurpoena pores WF 7 7% 06 1S oM12 2323 om Chelan tors WR 7 58 72 oma ava) om Dipodus sagas WE 7 a 08 Le oon? 3737 om otal group mea we 18 ome 20 40 oong nares raceaas—Palvgrapis marmortas WF 7 4% 4380 0012 162162 0% Maj puna WF 3, 6 Bo OMI? S13513 Expl rere WPS Wm 39 44 0012 Rone om anheporessa WF 1% a6 36 3636 3636 Om Toa group mean We W637 0 004d ShasEs ‘Table 3.Con Baie mae Tis oae we Taos ase “Gasropede Tepes cae WF sas 7397 ena daaod 1380 Pals. WF 8% 28 28 GOT? oHHe Or Tota group mean WE 6 ss e933 awd suman Tenors Peron vi WE 0% 305 3070873107108 AE ‘Cephalnpats cpus varie WES os 61 63 OSL 183183 0% Daal group mew WE TBs 43 Said 10761080 on 7 “paps pl ar 1 1or ‘ats oats a Sips toew Rr 2 Som oai2 2020 om Segihestenpt Rr 1 tom oai2 ota om Muraena blot Rr 4 100% oai2 oon w% Wada meanera Rr 2 oai2 oot o Sorpasapo (vei = 10m) RT $i omi2 oon om ‘Sip sia aul > 20) Rr 3 10% oad oat ro ‘Sopipaons orcas Rr car aig tana o% ‘Chelnlarcee Rr 2 om a2 223 om Diplo sas Rr 2 100% oai2 oon % Feta group ian Rr se ai 2223 on Tzatnoderms ——Poracenonr id Ros 00% ‘ay oor te ‘Ceplalpods tops wars ET 20%, ‘ag Tors Oe Geral group mean BURoe 386 7.2 199799 Osova ‘Time min Figure 3. Chromatogram of a sample of juvenile Sarpa salpa DT harvested in week 31 and analysed by LC-MS/MS, showing traces of p-PLTX ( 327.3, 1331.3 > 327.3, OsT-D 1318.3 327.3, 1309.3 + 327.3, OVTX-a 1324.3 + 327.3, 1315.3 + 327.3, OVIX-b 1346.3 > 371.2 1337.3 371.2 OVTX-c 1354.3 + 371.2 1345.3 + 371.2 OVTX-d 1332.34 3273 1323.3 327.3,