Concentration: 50 mg/ml change to 5 mg/ml (900 µl water + 100µl antibiotics)

20 µg/ml = 996 µl distilled water + 4µl antibiotics

40 µg/ml =992 µl distilled water + 8 µl antibiotics

60 µg/ml =988 µl distilled water + 12 µl antibiotics

80 µg/ml =984 µl distilled water + 16 µl antibiotics

100 µg/ml = 980 µl distilled water + 20 µl antibiotics

120 µg/ml = 976 µl distilled water + 24 µl antibiotics

140 µg/ml = 972 µl distilled water + 28 µl antibiotics

Kanamycin

M.W = 600.6 g/mol

Desired concentration = 5 mg/ml

5 𝑚𝑔/𝑚𝑙
Molar =
600.6 g/mol

= 0.0083 M / 0.0083 mol/L

Mass of Kanamycin required

Mass (g) = Concentration (mol/L) x Volume (L) x Formula Weight (g/mol)

= 0.0083 mol/L x 0.01L x 600.6 g/mol

= 0.0498g mix with 10 ml

20 µg/ml = 996 µl distilled water + 4µl antibiotics

40 µg/ml =992 µl distilled water + 8 µl antibiotics

60 µg/ml =988 µl distilled water + 12 µl antibiotics

80 µg/ml =984 µl distilled water + 16 µl antibiotics

100 µg/ml = 980 µl distilled water + 20 µl antibiotics

120 µg/ml = 976 µl distilled water + 24 µl antibiotics

140 µg/ml = 972 µl distilled water + 28 µl antibiotics

Streptomycin

M.W = 1457.4 g/mol

Desired concentration = 5 mg/ml

5 𝑚𝑔/𝑚𝑙
Molar = 1457.4 g/mol

= 0.00343 M / 0.00343 mol/L

Mass of Streptomycin required

Mass (g) = Concentration (mol/L) x Volume (L) x Formula Weight (g/mol)

= 0.00343 mol/L x 0.01L x 1457.4 g/mol

= 0.0499g mix with 10 ml

20 µg/ml = 996 µl distilled water + 4µl antibiotics

40 µg/ml =992 µl distilled water + 8 µl antibiotics

60 µg/ml =988 µl distilled water + 12 µl antibiotics

80 µg/ml =984 µl distilled water + 16 µl antibiotics

100 µg/ml = 980 µl distilled water + 20 µl antibiotics

120 µg/ml = 976 µl distilled water + 24 µl antibiotics

140 µg/ml = 972 µl distilled water + 28 µl antibiotics