736 APPENDIXES G MPN TABLES AND THEIR USE ‘When three serial sample volumes (e.g., dilutions) are used in the bacteriological testing of water, the resulting MPN values per 100 mL can be determined using Table G.1. The MPN values given there are based. on serial sample volumes of 10, 1, and 0.1 mL. If lower or higher ‘serial sample volumes are used, the MPN values given in Table G.1 must be adjusted accordingly. For example, if the sample volumes used_are 100, 16, and 1 mL, the MPN values from the table are multiplied by 0.1. Similarly, if the sample volumes are 1, 0.1, and 0.01 mL the MPN values from the table are multiplied by 10. In situations where more than three test dilutions have been run, the following rule is applied to select the three dilutions to be used in determining the MPN value [G.1]: Choosé-the highest dilution that gives positive results in all five portions tested-(no lower dilution giving any negative results) and the two next higher dilutions, Use the results at these three volumes in computing the MPN value. In the examples given in the accompanying table, the significant dilution results are shown in boldface. The number in the numérator represents positive tubes, that in the denominator, the total tubes planted. 01 001 0.001 Combination“ MPN/ Example ml = mk mL mL of positives 100 ml @ 5/5 8/5 2/5 O/S 520 4900 ) 5/3 4/5 2/5 OS 542 2200 © o/s 1/5 0/5 0/5 010 18 2 @ GS /atcra/Saecyseant/s: © 5/5 3/5 2/5 OS 532 1400 In example (c), the first three dilutions are used so as to throw the “positive result in the middle dilution. Where positive results occur in dilutions higher than the three chosen according to the above rule, they are incorporated into the result of the highest chosen dilution up_to a total of five, The result of applying this procedure to the data of example (4) is shown in example (¢). REFERENCE G.1 Standard Methods for the Examination of Water and Wastewater, (1980); 15th ed., American Public Health Association, New York.Ate tmttennnsnl TABLE G1 ‘Most Probable Number of Coliforms per 100 ml. of Sample NUMBER OF NUMBER OF NUMBER OF NUMBER OF NUMBER OF NUMBER OF POSITIVE TUBES _POSITIVETUBES _POSITIVETURES _POSITIVETUBES —POSITIVETUBES —_pOSITWVE RES i0 1 oa 1 1 on 10 1 1 1 1 Or 10 Oa io 102 me mp mL MPN’ mb ml mL MPN mL ml ml MPN ml mL ml MPN ml. mL ml MPN mt mt ot MPN 0 0 0 Paes dates reese Geter tee Eee see Cees eas reac eee © Pee Bee” ler eEnn © One? atEey EEaP nee oop RL Ot 40 2 0 1 68 3 0 1 on 4 0 1 tS OU Oe eee Oe OO 2 Oot go ogy gga ce ny 3 ee pote geese ascateaeOteeg are O0-t aes -ae Sue 1ostee Suaae as Seedy ghar teegaagiaiepe dee gree geeiguaaeiay Pepe aurea a ee ee ae ae ae ee ee Wee er re ee ee Die Diese rae ace ties Op Sue aM ete OHH SIHE LG Hea" ENG IEISHiog Ha aHHigitiaitiespriedet nie 95 Reece Oe tet O Oeste gee gg 33 ee gees arses Ceaeeeet est esu OL ae @eae Tere 2 es Saced ee seeg aseg asd sce ed eee aa ag ace eee eee ee erie eee HHI eeu g Henge eg egy eee aes aid 64 ee ee saree are Seg 1O ae eae sees. 14 a gece eee tee OD eaegtasa ace ecg ea 3 84 aeons ese ae ee ceed ae a ee ee Mca eg ag egg gga ee Oo. Suorisw 2 1 5 9 3 1 5 7 4 1 5-8 § 1 S$ 130 ; ee eee Oe ot eee Osa rote eg ee ge 49. fieceg Sea cueess ge tage eet ee 0.2 et cD eeu eee Seca eae cae ceca eae ae ge rede eee 70 | eet ere MO ee eee oe nA SI GCOIEE OH og eee Hera eget 95 sa sagem Aco ee aE ec De Seed eae giaeCDICee gue gta g age Heeger gniagit 120 Pye ee eee eee ate eee eet ter Meer eee ee er eer er Teter Geer eerie 150 Oe SB 12s 7 22 5m 3 25m 4625 0 5 2 180eee TABLEG.1. 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