SOP of taking venous blood specimens

A. Preparation tools
1. Condition of the prepared tools l is working well
2. The type of tools prepared should be suitable with action performed
The syringe tub include :
a. Disposable, single use, 21 gauge needle or 22 gauge needle
b. Disposable, single use, needle holder
c. Evacuated Blood Tubes
d. Tourniquet
e. Alcohol swab
f. Disposable gloves (non-latex)
g. Sharps Container
h. Pengalas
i. Sterile band aid /surgical tape/ paper adhesive tape
j. Handrub
k. Labolatory examination form
l. A label
3. Quantity of tools must be suitable with action
B. Orientation phase
1. Greeting the patient
2. Introduce yourself to your patient
3. Explain the reasons for venepuncture (how to take a venous blood sample)
4. Explain the procedure you are about to perform.
5. Asking patient's readiness
C. Working phase
1. Position Patient
The patient should either sit in a chair, lie down or sit up in bed. Hyperextend the
patient's arm.
2. Palpate patient’s arm for the best venipuncture site. Ask the patient to make a fist;
this helps to distend the veins. Choose the vein that feels the fullest. A good vein is
always palpable. It springs back like a small rubber band. If you have any doubt
about using the veins in one arm, check the other arm before starting the
venipuncture.
3. Put the pengalas under the arm to be drawn blood.
4. Wash your hands or disinfect with hand sanitizer.
5. Place the nierbekken on the pengalas
6. Put on a pair of disposable non-latex gloves
7. Apply the tourniquet 3-4 inches above the selected puncture site. Do not place too
tightly or leave it on more than 2 minutes. Patient should then make a fist without
pumping the hand.
8. Select a large well anchored vein, the medial cubital is the first choice. Trace the
patient's veins with your index finger. Tap the vein with your index finger to
encourage dilation then release tourniquet, and ask patient to open fist
9. Open the alcohol swab and clean potential venipuncture site, wiping with a circular
motion from the center to the periphery.