1 Magsombol, Frances Anne D.

| CHE 142-1

[E] = 0.06 mg/mL

Glucose

s s/v
80 529.8013
100 515.4639
300 779.2208
500 1408.451
750 1928.021
1000 2309.469
1400 3146.067
2 Magsombol, Frances Anne D. | CHE 142-1

Line Weaver
7
6
5
4
1/V

3 y = 351.68x + 1.9227
R² = 0.9718
2 1/s 1/v
1 0.0125 6.622517
0 0.01 5.154639
0 0.002 0.004 0.006 0.008 0.01 0.012 0.014 0.003333 2.597403
1/ [S] 0.002 2.816901
0.001333 2.570694
0.001 2.309469
Eadie Hofstee 0.000714 2.247191
0.5
0.45
0.4
0.35 y = -155.68x + 0.4937
0.3 R² = 0.8511
v/s v
0.25
V

0.001888 0.151
0.2
0.15 0.00194 0.194
0.1 0.001283 0.385
0.05 0.00071 0.355
0 0.000519 0.389
0 0.0005 0.001 0.0015 0.002 0.0025
0.000433 0.433
V / [S]
0.000318 0.445

Hanes Wolf Plot (Langmuir)

3500
3000
2500
s s/v
2000
y = 2.0303x + 318.74 80 529.8013
[S]/V

1500
R² = 0.9934 100 515.4639
1000
300 779.2208
500
500 1408.451
0
-500 750 1928.021
-500 0 500 1000 1500
[S] 1000 2309.469
1400 3146.067
3 Magsombol, Frances Anne D. | CHE 142-1

Since Langmuir Plot has highest r2, it is the best fit model. Hence,

For Langmuir:
[𝑆] 1 𝐾𝑚
= [𝑆] +
𝑉 𝑉𝑚𝑎𝑥 𝑉𝑚𝑎𝑥

1
Slope = 𝑉𝑚𝑎𝑥 = 2.0303

Vmax = 0.4925
𝐾𝑚
Y-intercept = 𝑉𝑚𝑎𝑥 = 318.74

Km = 156.9795

𝑉𝑚𝑎𝑥 0.4925
𝑘𝑐𝑎𝑡 = 𝐸𝑜
= 0.06
= 8.2083
𝐾𝐶𝑎𝑡 8.2083
𝐶𝑎𝑡𝑎𝑙𝑦𝑡𝑖𝑐 𝐸𝑓𝑓𝑖𝑐𝑖𝑒𝑛𝑐𝑦 = = 156.9795 = 0.05229
𝐾𝑀

Xylose

s s/v
20 62.5
50 95.96929
100 143.0615
300 270.2703
500 420.1681
700 526.3158
900 796.4602
4 Magsombol, Frances Anne D. | CHE 142-1

Line Weaver
3.5
3
2.5 1/s 1/v
2 0.05 3.125
1/V

1.5 0.02 1.919386

1 0.01 1.430615
y = 48.033x + 0.8046
0.5 0.003333 0.900901
R² = 0.9834
0 0.002 0.840336
0 0.01 0.02 0.03 0.04 0.05 0.06
0.001429 0.75188
1/ [S]
0.001111 0.884956

Eadie Hofstee
1.4
1.2
1 v/s v
0.8 0.016 0.32
V

0.6 0.01042 0.521

0.4 0.00699 0.699
y = -67.694x + 1.3124
0.2 R² = 0.9211 0.0037 1.11
0 0.00238 1.19
0 0.005 0.01 0.015 0.02 0.0019 1.33
V/ [S] 0.001256 1.13

Hanes Wolf Plot (Langmuir)

1000

800 y = 0.771x + 47.606

R² = 0.9827 s s/v
600
20 62.5
[S]/V

400 50 95.96929
200 100 143.0615
300 270.2703
0
-200 0 200 400 600 800 1000 500 420.1681
-200 700 526.3158
[S]
900 796.4602
5 Magsombol, Frances Anne D. | CHE 142-1

Since Lineweaver Plot has highest r2, it is the best fit model. Hence,

For Lineweaver:
1 𝐾𝑚 1 1
= +
𝑉 𝑉𝑚𝑎𝑥 [𝑆] 𝑉𝑚𝑎𝑥

1
y-intercept = = 0.8046
𝑉𝑚𝑎𝑥

Vmax = 1.2429

𝐾𝑚
Slope = 𝑉𝑚𝑎𝑥 = 48.033

Km = 59.7002

𝑉𝑚𝑎𝑥 1.2429
𝑘𝑐𝑎𝑡 = 𝐸𝑜
= 0.06
= 20.7142

𝐾𝐶𝑎𝑡 20.7142
𝐶𝑎𝑡𝑎𝑙𝑦𝑡𝑖𝑐 𝐸𝑓𝑓𝑖𝑐𝑖𝑒𝑛𝑐𝑦 = = 59.7002 = 0.3470
𝐾𝑀

a. Catalytic Efficiency
Glucose - 5.229%
Xylose - 34.70%

b. Specificity
Since Xylose has larger catalytic efficiency, the enzyme is therefore more efficient and more
specific to Xylose even though it could react with both as Glucose also has same characteristics as
the latter, it can also react with the enzyme.