Kurva Baku CTM

Hasil Absorbansi Sampel

Waktu Absorbansi Konsentrasi
(menit) 1 2 3 Rata-Rata
5 2,8055 2,6985 2,7061 2,7367
10 2,7877 7,000 3,0971 4,2949
20 1,7936 1,7081 1,7531 1,7516
30 2,9214 4,4899 4,4897 3,967
40
60
90

Perhitungan Konsentrasi Obat

Persamaan garis: y = 0,044x + 0,050
Faktor pengenceran = 10x
 Waktu 5 menit
y = 0,044x + 0,050
2,7367 = 0,044x + 0,050
0,044x = 2,7367– 0,050
2,6867
x=
0,044
x = 61, 061 ppm
Co = 61,061 mg/L x 0,25 L
= 15,265 mg x fp
= 15,265 mg x 10
 = 152,65 mg
152,65
Persen kadar = 𝑥 100% = 30, 53 %
500
5
FK = 250 x 152, 65 mg = 3,053

 Waktu 10 menit
y = 0,044x + 0,050
4,2949 = 0,044x + 0,050
0,044x = 4,2949– 0,050
4,244
x=
0,044
x = 96, 475 ppm
Co = 96, 475 mg/L x 0,25 L
= 24, 118 mg x fp
= 24, 118 mg x 10
= 241, 18 mg
Ct = Co + FK
= 241, 18 + 3,053
= 244, 240 mg
244,240
Persen kadar = 𝑥 100% = 48, 848 %
500
5
FK = x 244, 240 mg = 4, 884
250

 Waktu 20 menit
y = 0,044x + 0,050
1,7516 = 0,044x + 0,050
0,044x = 1,7516– 0,050
1,7016
x=
0,044
x = 38, 672 ppm
Co = 38,672 mg/L x 0,25 L
= 9, 668 mg x fp
= 9, 668 mg x 10
= 96,68 mg
Ct = Co + FK
= 96,68 + 4, 884
= 101, 565
101,565
Persen kadar = 𝑥 100% = 20, 313 %
500
5
FK = 250 x 101,565 mg = 2, 031

 Waktu 30 menit
 y = 0,044x + 0,050
3,967 = 0,044x + 0,050
0,044x = 3,967– 0,050
3,917
x=
0,044
x = 89, 022 ppm
Co = 89, 022 mg/L x 0,25 L
= 22, 255 mg x fp
= 22, 255 mg x 10
= 222,55 mg
Ct = Co + FK
= 222,55 + 2, 031
= 224, 58
224,58
Persen kadar = 𝑥 100% = 44,917 %
500
5
FK = 250 x 224,58 mg = 4, 491

 Waktu 40 menit
y = 0,044x + 0,050

 Waktu 60 menit
y = 0,044x + 0,050

 Waktu 90 menit
y = 0,044x + 0,050
PARAMETER FARMAKOKINETIK

𝑦2 −𝑦1
Slope =
𝑥2 −𝑥1

ln 61−ln 68
=
2−1,5

4,11−4,22
=
2−1,5

= -0,22 jam

Persamaan linier :
y = -0,22x + 4,55
Intersep = 4,55
Slope = 0,22

a. Konsentrasi awal
Dari persamaan linear , diperoleh intersep = ln 4,55
C0 = anti ln 4,55
= 94,63 ppm
= 94,63 μg/ml
b. Kecepatan eliminasi (K)
K = Slope
Slope = 0,22 jam

c. Volume Distribusi (Vd)

𝐷𝑜𝑠𝑖𝑠
Vd =
𝐶0

10000 𝜇𝑔
=
94,63 𝜇𝑔/𝑚𝑙

= 105,67 ml

d. Klirens (Cl)
Cl = Vd x k
= 105,67 ml x 0,22 jam
= 23,25 ml/jam

e. Waktu Paruh (t ½ )
0,693
t½ =
𝑘

0,693
=
0,22

= 3,15 jam