Internship POL

(Onsite)
Luci Guthey
Introduction

- Learn Sterile Technique in the Lab

- Understand Literature Reviews

- Keep up a Lab Notebook

- Keep my cells alive

Week #1

- Cleaned the fridge and -20 of Ion

Torrent boxes

- Organized and made lists of stuff in

the -80

- Made Media for cell culture

- Read grant proposal

Week #2 (Started cell culture)

Worked on my questions

- What are adherent cells?

- What are non adherent cells?
- Why are the conditions of the incubator set how they are?
- 37°C is equivalent to 98.6°F equal to your bodies
temperature.
- 5% Co2 to mimic what your body releases
 Week #3/ Week #4

- Continued Cell culture - Froze down DAP-3 Cells

DAP-3 Cells after being DAP-3 Cells after DAP-3 Cells after
put in a flask becoming 70% becoming 100%
confluent confluent
Literature Review

- Underlined what I thought was

important

- Boxes around what I should look

up

- List of questions
Lab Notebook

- What we did in the lab

- How each flask of cells was doing

- New protocols
Reflection

Success as an intern?

- My cells

Meaningful contribution?

- List of the things someone new in the lab should know

Meaningful to your education?

- Helped me realize I would love to work in a lab in the

future
Reflection

New appreciation?

- Understanding Literature

Important qualities or characteristics?

- Always knowing what is going on and what to do

Thank you
Any Questions?