Medio SOC

Compound 1000mL 500 mL 100 mL 50 mL

Bacto-yeast extract 5g 2,5 g 0,5 g 0,25

Bacto-tryptone 20 g 10 g 2g 1g
KCl 0,186 g 0,093 g 0,0186 g 0,0093 g
MgCl2 ó MgSO4 0,952 ó 2,408 g 0,476 ó 1,204 g 0,0952 ó 0,2408 g 0,0476 ó 0,1204 g

NaCl 0,584 g 0,292 g 0,0584 g 0,0292 g

Glucose 3,603 g 1,8015 g 0,3603 g 0,18015 g
H2O 1000 mL 500 mL 100 mL 50 mL

For maximum effectiveness, SOC media should have its pH adjusted to 7.0 by
adding concentrated sodium hydroxide.

Protocolo Realizado y estandarizado por Fabiola Rojas S