Professional Documents
Culture Documents
Research Design
The experimental group was composed of seven treatments and one replicate was
done for each treatment. The control group was composed of positive control
GENERAL PROCEDURE
from Tuap, Puguis, La Trinidad, Benguet. These were removed from the stems
and washed carefully. Then the leaf were cut into tiny pieces then underwent air
drying for an hour. These were then immersed separately in 95% ethanol for 48
hours as done by Augustine al (2005). After 48 hours, these were filtered using a
filter paper.
The filtered liquids were transferred into an evaporating dish for the
process of evaporation. The evaporation of the solvent was done through a water
bath until the liquid became syrupy to obtain a concentrated extract. The volumes
of the extracts were obtained using a graduated cylinder. Aliquots were taken to
12
Preparation of DPPH Solution
One and two tenths (1.2) milligrams of DPPH were dissolved in one
milliliter of 70% ethanol and subsequently diluted again with ten milliliters of 95
% ethanol.
spectrophotometer.
𝐴𝑏𝑠𝑏𝑙𝑎𝑛𝑘 −𝐴𝑏𝑠𝑠𝑎𝑚𝑝𝑙𝑒
Free radical inhibition = × 100%
𝐴𝑏𝑠𝑏𝑙𝑎𝑛𝑘
Where Absblank is the absorbance of the blank sample and Abssample is the
absorbance of the test samples. Inhibition of the free radical means how much free
13
Statistical Analysis of data
tested at 0.05 level of significance. When the computed value of F was greater
than the tabulated value, Duncan’s test was used to determine which treatments
are significantly different between the different concentrations and the positive
Disposal of Materials
Aseptic technique was used in the disposal of test organisms. The used
Celsius with a pressure of 15 psi. The autoclaved materials were cooled before
Figure 2 shows the flowchart of how the methods and procedures of this study
14
Gathering of Materials
Preparation of Materials
Mixing of Elaeagnus philippinensis and Selliguea taeniata leaf extracts and the
controls to the DPPH solution
Statistical Analysis
Conclusion
15