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Konsentrasi NH4 Transmitansi Absorbansi Chart Title

0.202 0.0228
0.1200
0.404 0.0280
0.606 0.0284
0.1000 f(x) = 0.0493063731x + 0.0032307582
0.808 0.0330 R² = 0.9161501822
1.01 0.0350 0.0800
1.212 0.0660
1.414 0.0830 0.0600
1.616 0.0912
0.0400
1.819 0.0924
2.021 0.1004
0.0200

0.0000
0 0.5 1 1.5 2
t Title

307582 C (ppm)

Xi Yi Xi2 Xi3

0.202 0.0228 0.040804 0.008242408

0.404 0.0280 0.163216 0.065939264
0.606 0.0284 0.367236 0.222545016
1.212 0.0792 0.571256 0.296726688

1.5 2 2.5
b2 bi
0.571256 1.212
0.296726688 0.571256
0.163166709 0.296726688

B2 -0.058817763
Bi 0.061386139
b0 0.0128

Y1 =
ai bi ci =
-0.05881776 0.0613861386 0.0128
A B c
-0.05881776 0.0120861386 0.0096

B^2 4*A*C B^2 - 4*A*C (B^2- 4*A*C)^0,5

0.000146075 -0.002258602 0.002404677 0.0490375045
Xi4 Xi*Yi Xi2 *yi

0.0016649664 0.0046056 0.00093

0.0266394627 0.011312 0.00457
0.1348622797 0.0172104 0.01043
0.1631667088 0.033128 0.01593

bo
3= 0.0792
1.212 = 0.033128
0.571256 = 0.01593

Y2
a2 b2 c2
0 0.0493 0.0032

-B2 +/- (B^2- 4*A*C)^0,5 2*A XI X2

0.0369513659 -0.1176355259 -0.31412
-0.0611236431 0.519602

C1 = ppm 0.519602
c2 = ppm -0.31412
 Kurva standart protein C (µg/ml)

Konsentrasi Protein (µg/ml) Transmitansi Absorbansi Xi

5 0.149
10 0.238 5
15 0.285 10
20 0.389 15
25 0.488 30
30 0.598
35 0.665 b2
40 0.782 350
45 0.904 4500
50 1.005 61250

Kurva Standart Protein a1

1.2 -0.00084
1.005
1 0.904
f(x) = 0.0191381818x + 0.024 A
ABSORBANSI

R² = 0.9942141937 0.782
0.8
0.598
0.665 -0.00084
0.6 0.488
0.389
0.4 0.285
B2
0.238 0.00012769
0.2 0.149

0
0 10 20 30 40 50 60

KONSENTRASI (ppm)
µg/ml)

Yi Xi2 Xi3 Xi4

0.149 25 125 625

0.238 100 1000 10000
0.285 225 3375 50625
0.672 350 4500 61250

bi b0
30 3 = 0.672
350 30 = 7.4
4500 350 = 91.65

y1 = y2
b1 c1 = a2
0.0304 0.018 0

B C
0.0113 -0.006

4*A*C B2 -4*A*C B2 -4*A*C^0,5 - B + / - (B2 -4*A*C^0,5)

0.00002016 0.00010753 0.0103696673 -0.0009303327
-0.0216696673
 Xi * Yi Xi2 *Yi

0.745 3.725
2.38 23.8
4.275 64.125
7.4 91.65

b2 -0.00084
bi 0.0304
b0 0.018

b2 c2
0.0191 0.024

2*A X1 X2
-0.00168 0.553769
12.898611

C1 (ppm) 12.89861
c2 (ppm) 0.553769
 Kurva Absorbansi
Konsentrasi Protein (µg/ml) Absorbansi
4 0.2682
6 0.2920
8 0.3005
10 0.4650
12 0.5561

Chart Title
0.6

0.5 f(x) = 0.03744x + 0.07684

R² = 0.8661695347

0.4

0.3

0.2

0.1

0
3 4 5 6 7 8 9 10 11 12 13

C actual Sloope Intercept Absorbansi t

4 0.03744 0.07684 0.2682
6 0.2920
8 0.3005
10 0.4650
12 0.5561

SD
SE of Intercrcept
n^0,5
SD of Intercept
SD of Intercept/slope
LOD
LOQ
 C (µg/ml)

Xi Yi Xi2
4 0.2682 16
6 0.292 36
8 0.3005 64
18 0.8607 116

b2 bi b0
116 18 3
792 116 18
5648 792 116

b2 -0.0019125
bi 0.031025
b0 0.1747

y1
a1 b1 c1
12 13
-0.0019125 0.031025 0.1747

A B C
-0.0019125 -0.006375 0.0979

B2 4*A*C B2 -4*A*C
4.0640625E-05 -0.000748935 0.0007895756

Sloope Intercept
0.03744 0.07684

C(Konsentrasi) terukur % Recovery n^0,5

5.11111111 127.777777778 2.2360679775
5.74679487 95.7799145299 2.2360679775
5.97382479 74.6728098291 2.2360679775
10.36752137 103.675213675 2.2360679775
12.80074786 106.67289886 2.2360679775

SD 19.1976357296
SE of Intercrcept
n^0,5
SD of Intercept
SD of Intercept/slope
LOD
LOQ
 Xi3 Xi4 Xi * Yi Xi2 *Yi
64 256 1.0728 4.2912
216 1296 1.752 10.512
512 4096 2.404 19.232
792 5648 5.2288 34.0352

= 0.8607
= 5.2288
= 34.0352

= y2
= a2 b2 c2
0 0.0374 0.0768

B2 -4*A*C^0,5 - B + / - (B2 -4*A*C^0,5) 2*A X1 X2

0.0024342473 -0.0024748879 -0.003825 0.64703
-0.0088092473 2.3030711806
C1 ppm 2.303071
C2 ppm 0.64703
Konsentrasi Protein (µg/ml) Absorbansi Slope Intercept Xi
4 0.2682 0.03744 0.07684 3.49037
6 0.2920 0.03744 0.07684 3.800104
8 0.3005 0.03744 0.07684 3.910724
10 0.4650 0.03744 0.07684 6.051536
12 0.5561 0.03744 0.07684 7.237116
(Xi-X)^2
-12.5096
-32.1999
-60.0893
-93.9485
-136.763
-335.51