● Introduction.

Immunoglobulin structure, generation by recombination of gene segments; Antibody

engineering: Genetic engineering vs. Chemical engineering

● Rekombinant antibody formats and their uses I. Generation of various antibody fragments,
oligomerization strategies.

● Rekombinant antibody formats and their uses II. Humanization; design of effector functions – Fc
engineering, whole antibodies and their uses.

● Alternatives to antibodies and fields of application Rekombinant antibodies in proteomics, protein

chips (protein microarrays).

● Bioinformatics for rekombinant antibody design sequence analyss, database searches (imgt,
PubMed), analysis of V region usage.

● Phage display - invited speaker: Gábor Pál Bakteriophage technologies in immunology, basics of
phage display and its potential uses in immunology.

● Human immunoglobulin production in transgenic and transchromosomal mice and cattle. – invited
speaker: Imre Kacskovics Production and relevance of polyclonal human antibodies, human
monoclonal antibodies from mice, the role of FcRn in immunotherapy.

● Therapeutic recombinant antibodies and their clinical useI.– autoimmune disorders : Péter Gergely j.
Possibilities of monoclonal antibody therapy for the treatment of rheumatoid arthritis.– neoplastic
disorders: András Rosta Strategies for the treatment of neoplastic disorders using monoclonal
antibodies.

● Design and generation of soluble receptors - invited speaker: István Kurucz natural soluble receptors
and their function, solubilization and characterization of membrane receptors, expression strategies

● Therapeutic recombinant antibodies and their clinical use II. Industrial scale production of
antibodies, general strategies and requirements, the long way from genes to drugs.

● Recombinant vaccines modified pathogens, recombinant proteins, DNA vaccines.

● Multiplex measurement techniques Cytometric bead arrays in immunology

● Nanobeads in immunology immunological properties of nanobeads, interactions with macrophages

and dendritic cells, nanobead targeting

● Cell therapy in immunology Hematopoetic stem cells and gene therapy for the treatment of
immunologic disorders

 Antibody Engineering : Methods and Protocols Author(s): Lo, Benny K. C. Format:

Hardcover
 Humana Press, 2004, DOI: 10.1226/1588290921
 Recombinant Antibodies Frank Breitling, Stefan Dübel, John Wiley, 1999, ISBN: 978-0-471-
17847-7 Paper