Media: Proteus mirabilis (Ttal Inhibition)

1. Cefoxitin Cycloserine Fructose Agar

(CCFA) 2. Clostridium difficile (Fair to heavy
growth)
2. Cefoxitin Cycloserine Egg-Yolk Agar Escherichia Coli (Total Inhibition)
(CCEY) Clostridium perfringens (Total Inhibition)
Escherichia coli (Total Inhibition)
Proteus mirabilis (Ttal Inhibition)
Component:
1. Proteose peptone
Disodium hydrogen phosphate Characteristics:
Potassium dihydrogen phosphate 1. The colony and surrounding medium
Magnesium sulphate change
Sodium chloride color from rose to yellow as the pH rises.
Fructose Mannitol is utilized by fewer Clostridium
Agar species than fructose and improves the
D-cycloserine recovery of C. difficile. Cefoxitin and
Cefoxitin cycloserine are incorporated to inhibit
Defibrinated horse blood normal fecal flora.

2. Peptone Mix 2. Characteristics of C. difficile: Gray/white,

Sodium chloride opaque, flat colonies, 1.5-3mm diameter,
Soluble Starch circular but may be elongated in the
Agar direction of spread, ground glass appearance
Sodium bicarbonate and a rough, fimbriate edge. Lecithinase
Glucose negative. Incubation longer than 48hrs may
Sodium pyruvate result in a lighter gray or white centre to the
Cysteine HCl colony. Phenolic odour due to the
Haemin production of p-cresol. Colonies fluoresce
Vitamin K yellow/green under UV light.
L-arginine
Soluble pyrophosphate
Sodium succinate Specimen:
Cholic acid 1. Stool
p-Hydroxyphenylacetic acid
D-Cycloserine 2. Stool
Cefoxitin
Egg yolk emulsion
Lysed horse blood Gram Reaction:
1. Have a broad range of antimicrobic
activity against aerobic, anaerobic and
Organisms: facultatively anaerobic gram-postive and
1. Clostridium difficile (Fair to heavy gram-negative bacteria while permitting
growth) recovery of C. difficile. C. difficile
Clostridium perfringens (Total Inhibition) colonies produce yellow fluorescence
Escherichia coli (Total Inhibition) when viewed with long-wave UV light.
2. Selective media for isolation of
Clostridium difficile form suspected cases of
pseudomembranous colitis/antibiotic-
associated diarrhea.