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ABSTRACT: Purple passion fruit were processed by flash vacuum-expansion in comparison with a single-strength
juice. A puree was obtained with about 50% / fruit weight yield, which is 2-fold that obtained for the reference juice.
Color and cell-wall polysaccharides of the products were analyzed, and their rheological properties were investi-
gated. The red-purple puree was enriched in anthocyanins and alcohol-insoluble residue. The puree had higher
consistency and viscosity, which was related to its alcohol-insoluble residue and starch contents.
Key Words: purple passion fruit, vacuum-expansion, puree, cell walls, viscosity
Introduction Materials and Methods (1) fruit (1 kg) were placed in the steam-
542 JOURNAL OF FOOD SCIENCE—Vol. 66, No. 4, 2001 © 2001 Institute of Food Technologists
Preparation of Passion-Fruit Puree . . .
Table 3—Yield and composition of water-soluble polysaccharides sion step was evidenced by the higher
Sample content of the red-purple vacuum-ex-
panded puree than that obtained from
Reference Steam-heated Vacuum-expanded
juice puree puree steam-heated fruit. In agreement with
previous reports (Cogat 1994, 1995), the
Non-starchy water-soluble 0.1 0.6 0.9 vacuum-expansion must loosen the tis-
polysaccharides (%/fresh weight)
sue cohesion by creating microchan-
Composition of non-starchy
nels, thus separating cells at their cell-
water-soluble polysaccharides
(%/dry weight) wall level and facilitating extraction of
Uronic acids a 28.0 63.2 68.3 color.
Neutral non-cellulosic 25.8 8.5 6.6 The single-strength juice had high L
and b values (Table 1), indicating a clear
polysaccharides b
and yellow appearance as usually ob-
Proteins (N x 6.25) — 12.4 10.7
served (Casimir and others 1981). The
Methanol c 2.0 (40.1) 9.9 (86.5) 9.9 (79.7)
orange-yellow color of passion-fruit
Uronic acids d 48.7 86.4 89.9
juice is due to carotenoids, mostly b-
Rhamnose d 1.5 1.0 1.3
carotene (Nakasone and Paull 1998) lo-
Fucose d 0.5 0.1 0.2 cated in the aril portion of the fruit.
Arabinose d 9.8 2.8 2.0 Pulping by itself did not degrade the
Xylose d 3.2 1.7 0.8 purple mesocarp. The purees from
Mannose d 5.9 1.0 0.9 steam-heating or flash vacuum-expan-
Galactose d 25.8 5.6 3.7 sion exhibited higher a values and lower
Glucose d 4.5 1.2 1.2 L and b values than the reference juice,
h (mL/g) — 111 127 with respectively orange-red and red-
a Expressed as ‹‹anhydrogalacturonic acid›› purple appearance.
b Neutral polysaccharides obtained by hydrolysis with diluted acid (TFA or sulfuric acid) and GC of the
The pulp contents (360 3 g; 10 min)
alditol acetates and expressed as sum of ‹‹anhydrosugars››
Table 4—Rheological characteristics of passion-fruit processed products were present in similar proportions.
Sample Thus the specific effect of the flash
vacuum-expansion step against the
Vacuum-
Vacuum- expanded steam-heating step is again evident. It is
Reference Steam-heated expanded puree + possible that the vacuum-expansion
juice puree puree aromatic liquors provokes disintegration of additional
Bostwick consistency — 6.0 1.7 3.7 tissues, richer in cellulose and xylose-
(cm in 30 s) containing polysaccharides, with re-
Apparent viscosity (Pa.s) 2.4 6 0.3 33.0 6 1.4 54.8 6 4.1 045.2 6 1.5 gards to the steam-heating only. A sepa-
Yield stress (Pa) 2.1 6 0.3 72.8 6 2.1 188.7 6 11.7 103.6 6 5.2 rate analysis of cell walls from en-
Reconstituted puree docarp and mesocarp (exocarp was not
damaged) would be needed to ascertain
Alcohol insoluble 3.2 4.8 6.3 —
residue (%/fresh weight) this hypothesis.
Apparent viscosity (Pa.s) 2.4 6 0.3 19.1 6 0.8 51.0 6 3.7 — Water-soluble polysaccharides (WSPs)
Destarched alcohol 0.6 2.3 3.9 — were extracted from the destarched alco-
insoluble residue (%/fresh weight) hol-insoluble residues with water at am-
Apparent viscosity (Pa.s) — 6.5 6 0.4 38.2 6 2.4 — bient temperature. Again, their levels in-
a Significance of differences was defined at p < 0.05.
creased from reference juice to vacuum-
expanded puree (Table 3). They repre-
sented, respectively, 26% and 23% of the
portions of rhamnose and fucose. The and galactose. The residue from the vac- destarched alcohol-insoluble residues
residue from the reference juice had a uum-expanded puree was richer in cellu- from steam-heated and vacuum-expand-
composition very different from the 2 lose and xylose-containing polysaccha- ed purees. According to their polysaccha-
others, having relatively lower propor- rides and poorer in uronic-acid-contain- ride composition, WSPs from steam-
tions of uronic acids and cellulose and ing polysaccharides than that from the heated and vacuum-expanded purees are
much higher proportions of arabinose steam-heated puree. Other neutral sugars mainly pectic substances with dominant
Food Engineering and Physical Properties
Rheological Characteristics
Consistencies of the purees were
measured with a Bostwick consistome-
ter (Table 4). The consistency of the pu-
ree from the flash-expanded fruit was
more than 3-fold higher than that of
the puree from steam-heated fruit. This
increase cannot be explained by the
concentration due to the vacuum-ex-
pansion (loss of about 10% of water)
because, even after reincorporation of
aromatic liquors, the consistency was
still far higher than after simple steam-
heating.
Apparent viscosity (Table 4) in-
creased similarly to the consistency:
Figure 3—HPSEC profile of water-soluble polysaccharides (a: reference juice,
b: steam-heated puree, c: vacuum-expanded puree) on a Shodex OHpak SB- Vacuum-expanded puree exhibited
804 HQ column in 0.1M LiNO3. Elution times of pullulan standards (P5 ➜ P800) higher viscosity than steam-heated pu-
are also shown. ree and also after reincorporation of
aromatic liquors. Purees from vacuum- the viscosity of vacuum-expanded pas- method for the analysis of sugars in plant cell-wall
polysaccharides by gas-liquid chromatography.
expanded fruit, even with reincorpora- sion-fruit puree is mainly due to (about Carbohydr Res 16:127-150.
tion of aromatic liquors, had far higher 75%) its destarched alcohol-insoluble Askar A, Treptow H. 1993. Measurement of colour. In:
Askar A, Treptow H, editors. Quality assurance in
yield stresses than the puree from residue content. tropical fruit processing. Berlin, Germany:
steam-heated fruit (Figure 4). Yield Springer-Verlag. p 57-60.
stress of vacuum-expanded puree was Conclusions Bietz JA. 1974. Micro-Kjeldahl analysis by an im-
proved automated ammonia determination fol-
similar to peach puree (Lozano and
Ibarz 1994). The different fluids had a
shear-thinning non-Newtonian behav-
T HE FLASH VACUUM -EXPANSION PRO -
cess, when applied to purple pas-
sion fruit, extends its possible uses by
lowing manual digestion. Anal Chem 46:1617-
1618.
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simple and rapid preparation of alditol acetates
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studied by reconstituting model disper- aroma compounds of this product in Casimir DJ, Kefford JF, Whitfield FB. 1981. Technolo-
sions of the residues in water-sucrose comparison with the juice itself. gy and flavor chemistry of passion fruit juices and
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mixture. Apart from the puree from the Chan HTJR. 1993. Passion fruit, papaya and guava
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