ABSTRACT

Irawati, Santia. 2018. Profiling The Activity of Anti-Cancer Against Breast

Cancer (T47D) and Toxicity Towards Normal Cells (Vero) in White
Chrysanthemum (Chrysamthemum cinerariifolium (Trev.)). Thesis. Study
Program Pharmacy Faculty of Medicine and Health Sciences State Islamic
University Maulana Malik Ibrahim Malang.

Advisor: Drg. Anik Listiyana, M. Biomed

White chrysanthemum (Chrysanthemum cinerariifolium (Trev.)) is one of

the medicinal plants that has empirically been used by the community as a cure to
various types of diseases; one of them known as anticancer. The aim of this study
was to investigate the differences in anticancer activity and toxicity of flowers,
leaves, stems and roots of chrysanthemum (C. cinerariifolium (Trev.)) against
breast cancer cells (T47D cell line) and normal cells (Vero cell line). The
extraction method used is called Ultrasonic-Assisted Extraction (UAE). Each
extract was tested for anticancer activity against T47D cells and toxicity test
against Vero cells using MTT method. The results showed that the extract of
flowers, leaves, stems, and roots of C. cinerariifolium had different anticancer
activity against T47D cells with IC50 (P <0.000) values, 772,94; 362,58; 168.46;
and 293,81 μg / mL. Besides, C. cinerariifolium has different toxicity to Vero cell
with the value of CC50 (P <0.009), consecutively; 701,46; 676,18; 569.18; and
728.19 μg / mL, and has SI value of 0.91; 1.86; 3.38; and 2.48. The extract that
was declared and known to be selective in killing breast cancer cells (T47D) and
safe against normal cells, were stem extracts with SI> 3 and IC50 values of 168.46
μg / mL.

Keywords: C. cinerariifolium, activity, toxicity, T47D cells, and Vero cells