Animal-free Expression

Plant expression systems are used when animal-free recombinant protein products
are required. Compared to traditional microbial or animal cell systems, the
animal-free plant expression systems greatly reduce the risk of contamination with
animal-origin pathogens. Furthermore, plant production possesses superior
advantages in storage, distribution and scale-up.

Many plant species have been developed as protein expression systems, such as
rice, tobacco, and Arabidopsis thaliana. To obtain transgenic plants for the stable
expression of proteins, plant virus and bacterium are utilized to deliver the desired
gene into plant hosts. The most commonly used delivery systems include tobacco
mosaic virus (TMV) and Agrobacterium.

Animal-free expression system can be used to express large quantities of proteins. Many plant species
are developed as protein expression systems, such as tobacco, rice and Arabidopsis thaliana.

Profacgen provides a high capacity rice grain (Oryza sativa L.) expression system.
It is a robust and flexible expression system that allows the expression of both
short peptides (shorter than 100 amino acids) and large recombinant proteins (up
to 1000 amino acids). The rice grain system uses rice endosperm cells as the host
and rice farming for scaling up production.
 The rice endosperm specific expression system

Features
High yield (from 2g/kg to 10g/kg of rice seed)
High scale-up capability
Easy storage and distribution (rice seed containing target protein or peptide can
be stored for many years without compromising the bio-activity)
Validated highly consistent performance
Free of infectious or toxic contaminants
Cost-effective
Environment friendly

Services

1. Protein Expression

Phase Service
Phase Service

Phase 1: Generation of genetic Gene optimization and synthesis, vector construction

engineering plants

Generation of transgenic plants (T0, 20~50 plants)

Screening for high expression lines

Phase 2: Lab scale Screening the genetic stability of homozygous genetic

engineering lines

Genetic analysis of genetic engineering line

Detection and activity analysis of recombinant protein

Developing a protocol for purification of the protein

providing a microgram-scale with 90-95% purity

Phase 3: Pilot scale Porviding the minigram or gram protein with 90~95% pur

Providing the data for biosafety assessment of transge

plant

2. Developing a protocol of pilot and large scale processing and manufacturing

3. Developing analytic methodology and methodology validation

Profacgen provides you with an ultimate solution of animal-free protein

production. Our service can be tailored according to your specialized requirements
(codon optimization, tag selection, purity etc.). Our expertise guarantees high
protein quality, short turnaround time, and the best price in the market!