Retensi AUC

Blangko pelarut
sampel 2ml 3.425 122.51
sampel + 48ppm 3.433 149.123
sampel + 48ppm 3.422 148.798
sampel + 48ppm 3.391 148.508
sampel + 48ppm 3.391 149.107
sampel + 48ppm 3.491 149.071
sampel + 48ppm 3.394 149.399
sampel + 60ppm 3.381 155.768
sampel + 60ppm 3.422 155.759
sampel + 60ppm 3.413 155.709
sampel + 60ppm 3.411 155.761
sampel + 60ppm 3.424 155.481
sampel + 60ppm 3.412 155.18
sampel + 72ppm 3.422 162.508
sampel + 72ppm 3.413 161.071
sampel + 72ppm 3.411 161.721
sampel + 72ppm 3.427 162.287
sampel + 72ppm 3.399 161.401
sampel + 72ppm 3.425 161.481
20ppm parasetamol 3.423 8.741
20ppm parasetamol 3.433 8.744
20ppm parasetamol 3.415 8.739
40ppm parasetamol 3.422 18.129
40ppm parasetamol 3.416 18.133
40ppm parasetamol 3.424 18.131
60ppm parasetamol 3.412 25.509
60ppm parasetamol 3.422 25.511
60ppm parasetamol 3.413 25.523
80ppm parasetamol 3.411 32.101
80ppm parasetamol 3.427 31.999
80ppm parasetamol 3.422 32.319
100ppm parasetamol 3.411 42.074
100ppm parasetamol 3.424 42.077
100ppm parasetamol 3.412 42.052
120ppm parasetamol 3.413 50.567
120ppm parasetamol 3.411 50.671
120ppm parasetamol 3.398 50.701

metanol: air (6:4)

deteksi= 243nm
laku alir= 1ml/menit
kolom = C18
Konsentrasi AUC
20 ppm PCM 8.741
20 ppm PCM 8.744
20 ppm PCM 8.739
40 ppm PCM 18.129
AUC
40 ppm PCM 18.133 60
40 ppm PCM 18.131
50
60 ppm PCM 25.509 f(x) = 0.4113166667x + 0.7414444444
R² = 0.9969154674
60 ppm PCM 25.511 40
60 ppm PCM 25.523
80 ppm PCM 32.101 30
80 ppm PCM 32.101
20
80 ppm PCM 32.101
100 ppm PCM 42.074 10
100 ppm PCM 42.077
100 ppm PCM 42.052 0
120 ppm PCM 50.567 0 20 40 60 80 100 120 1

120 ppm PCM 50.671

120 ppm PCM 50.701

Konsentrasi AUC
20 8.741333333
40 18.131
60 25.51433333
80 32.101
100 42.06766667
120 50.64633333
100 120 140
PPM AUC (Y) RATA - RATA AN (Y – Yi)2
20 8.741 8.9674 0.05125696
40 18.129 17.1934 0.87534736
60 25.509 25.4194 0.00802816
80 32.101 33.6454 2.38517136
100 42.074 41.8714 0.04104676
120 50.567 50.0974 0.22052416
3.58137476
Y = 0.4113X + 0.7414

SD = 1.8924520496
0.3784904099

LOD = 1.1354712298
2.760688621

LOQ = 3.7849040992
9.202295403
Kons. Teoritas AUC Kons. Didapati (ppm) RATA - RATA
Sampel + 48 ppm 149.123 361.1453261928
Sampel + 48 ppm 148.798 360.3541869523
Sampel + 48 ppm 148.508 359.6482473223
Sampel + 48 ppm 149.107 361.1063777994
Sampel + 48 ppm 149.071 361.0187439143
Sampel + 48 ppm 149.399 361.8171859786 2.0617932616
Sampel + 60 ppm 155.768 377.3210808179
Sampel + 60 ppm 155.759 377.2991723466
Sampel + 60 ppm 155.709 377.1774586173
Sampel + 60 ppm 155.761 377.3040408958
Sampel + 60 ppm 155.481 376.6224440117
Sampel + 60 ppm 155.81 377.4233203505 2.4761086711
Sampel + 72 ppm 162.508 393.7280915287
Sampel + 72 ppm 161.071 390.2300389484
Sampel + 72 ppm 161.721 391.8123174294
Sampel + 72 ppm 162.287 393.1901168452
Sampel + 72 ppm 161.401 391.0333495618
Sampel + 72 ppm 161.481 391.2280915287 2.4648203186

Y = 0.4108X + 0.7645