[S](M) v (u mol/min) 1/S 1/v

0.0005 125 2000 0.008

0.0002 125 5000 0.008
0.00006 121 16666.66667 0.0082644628
0.00004 111 25000 0.009009009
0.00003 96.5 33333.33333 0.0103626943 0.14
0.00002 62.5 50000 0.016
1.60E-05 4.27E+01 62500 0.0234192037 0.12
0.00001 13.9 100000 0.071942446
0.1
8.00E-06 7.5 125000 0.1333333333
0.08

1/V
Lineweaver-Burk Plot 0.06
1/v = (Km/vm)(1/S) + (1/vm) 0.04
sb y = (slope) (sb x) + intercept
0.02
slope = 9.00E-07
0
intercept = -0.0111
-10000 1000

(1/vm) = intercept
vm = -90.0900900901

Km/vm = slope
km = -8.11E-05
 Plotting Lineweaver-Burk
0.14

0.12

0.1 f(x) = 9.26229014192706E-07x - 0.0111357691

R² = 0.8357517679
0.08
1/V

0.06

0.04

0.02

0
-10000 10000 30000 50000 70000 90000 110000 130000
1/S
[S] (M) v (u mol/min) 1/S 1/v ln [S] ln(v/vm-v)
0.0005 125 2000 0.008 -7.6 4.83
0.0002 125 5000 0.008 -8.51 4.83 G
0.00006 121 16666.67 0.008264 -9.72 3.19
0.00004 111 25000 0.009009 -10.12 2 f(x) = 1.94935644
R² = 0.914987747
0.00003 96.5 33333.33 0.010363 -10.41 1.18
0.00002 62.5 50000 0.016 -10.82 -0.016
1.60E-05 4.27E+01 62500 0.023419 -11.04 -0.67

ln (v/vm-v)
0.00001 13.9 100000 0.071942 -11.51 -2.09
8.00E-06 7.5 125000 0.133333 -11.73 -2.76 -12 -11.5 -11
 Grafik Enzim Alosterik
6
f(x) = 1.9493564478x + 20.9757934128
R² = 0.9149877477 5
4
3
ln (v/vm-v)

2
1
0
-12 -11.5 -11 -10.5 -10 -9.5 -9 -8.5 -8 -7.5 -7
-1
-2
-3
-4
ln [S]
S (M) v (u mol/min) v/S
0.0005 125 250000
0.0002 125 625000
0.00006 121 2016667
0.00004 111 2775000
0.00003 96.5 3216667
0.00002 62.5 3125000 3500000
1.60E-05 4.27E+01 2668750
0.00001 13.9 1390000 3000000
8.00E-06 7.5 937500
2500000

2000000
Eadie-Hofstee Plot f(x) = - 1356.2846128693x + 1995655.5
v/S = (vm/Km) - (1/Km) v 1500000
Sb y = (intercept) - (slope) sb x
1000000
slope = -1356.3
intercept = 2.00E+06 500000

0
(-1/Km) = slope 0 20 40 60 80 100 120 14
Km = 0.0007373

vm/Km = intercept
vm = 1.47E+03
6.2846128693x + 1995655.51265194 Column C
Linear (Column C)

60 80 100 120 140

S (M) v (u mol/min) S/v
0.0005 125 0.000004
0.0002 125 1.6E-06
0.00006 121 4.96E-07
0.00004 111 3.6E-07
0.00003 96.5 3.11E-07
0.00002 62.5 3.2E-07 0.0000045
1.60E-05 4.27E+01 3.75E-07
0.000004
0.00001 13.9 7.19E-07
f(x) = 0.0071076
8.00E-06 7.5 1.07E-06 0.0000035

0.000003
Hanes-Woolf Plot
S/v = (Km/vm) + (1/vm) (S) 0.0000025
Sb y = intercept + (slope) (sb x) 0.000002

0.0000015
slope = 0.0071
intercept = 3.00E-07 0.000001

0.0000005
1/vm = slope
0
vm = 140.8450704225
0 0.00010.00020.

km/vm = intercept
km = 4.23E-05
000045

000004
f(x) = 0.0071076907x + 3.29412088131039E-07
000035

000003

000025
Column C
000002 Linear (Column C)
000015

000001

000005

0
0 0.00010.00020.00030.00040.00050.0006
S (M) v (u mol/min) log S V/Vm-V
0.0005 125 -3.3010299957 0.092936803
0.0002 125 -3.6989700043 0.092936803
0.00006 121 -4.2218487496 0.0896960712
0.00004 111 -4.3979400087 0.0816777042
0.00003 96.5 -4.5228787453 0.0702584638
0.00002 62.5 -4.6989700043 0.0444049734
1.60E-05 4.27E+01 -4.7958800173 2.99E-02
0.00001 13.9 -5 0.0095460477
8.00E-06 7.5 -5.096910013 0.0051282051

Allosteric Enzyme
ln v/vm-v = n (ln S) - ln Km -5.2 -5 -4.8 -4.6 -4.4 -4.2 -4 -3

Vm = 1.47E+03
Basis = 10
f(x) = 0.6079941068x + 1.2910424488

-12 -11.5 -11 -10.5 -10 -9.5 -9

f(x) = 0.6079941068x + 2.9727350969

 Log (v/vm-v) Ln S Ln (v/vm-v)
-1.0318122713 Slope = 0.608 -7.6009024595 -2.37584
-1.0318122713 Intercept = 1.291 -8.5171931914 -2.37584
-1.0472265794 -9.7211659957 -2.41133
-1.0878964779 -10.1266311039 -2.50497
-1.1533013504 -10.4143131763 -2.65557
-1.3525683862 -10.8197782844 -3.1144
-1.5240873908 -11.0429218357 -3.50934
-2.0201764016 -11.512925465 -4.65163
-2.2900346114 -11.7360690163 -5.273

0
.6 -4.4 -4.2 -4 -3.8 -3.6 -3.4 -3.2

-0.5

068x + 1.2910424488
-1
Column E
Linear (Column E)
-1.5

-2

-2.5

0
10.5 -10 -9.5 -9 -8.5 -8 -7.5 -7

-1

1068x + 2.9727350969
-2

Column J
-3
Linear (Column J)

-4

-5

-6
-5

-6