Example 6.1, A strain of mold was grown in a batch culture on glucose and the following data were ob- tained. call Giucore ‘Time concentration concentration oO (e) em ° 125 100 9 2s 97 6 51 904 2B wos 69 30 2 481 a4 3 26 36 375 9.38 40 an 063 a, Calculate the maximum net specific growth rate, », Calculate the apparent growth yield. What maximum cell concentration could one expect if 150 g of glucose were used with the same size inoculum? Example 6.2. Logistic Equation Ethanol formation from glucose is accomplished in a batch culture of Saccharomyces cere- visiae, and the following data were obtained. Time (h) Glucose (S), g/L Biomass (X), gL Ethanol (), g/l. 0 100 os 00 2 9s 10 2s 5 85 2 15 10 58 48 200 15 30 1 340 20 2 96 430 23 3 104 415 0 2 107 490 a By fitting the biomass data to the logistic equation, determine the carrying-capacity coefficient 1b, Determine yield coefficients Ypys and Yys.Example 6.4. A now strain of yeast is being considered for biomass production. The following data were obtained using a chemostat. An influent substrate concentration of $00 mg/l and an excess of oxygen were used at a pH of 5.5 and T= 35°C. Using the following data, calculate fly, Ky, Vil, ky, and m,, assuming jt, = lS, +S) — ky. (Carbon substrate Celt Dilution rte ¢h') concentration (mg/!) concentration (mg/t) ou 167 366 02 335 407 03 504 408 os 101 404 os 169 37 06 298 299 07 m2 59 Example 6.5. ‘The specific growth rate for inhibited growth in a chemostat is given by the following equation: —— nS _ K,+S+IK JK, @) where cells ) 0.05 ob S=10eh R= Tell 1 l imbs Xo=0 Kj=001 gl y= 05h a, Determine X and $ as a function of D when T= 0. b, With inhibitor added to a chemostat, determine the effluent substrate concentration and X as a function of D. . Determine the cell productivity, DX, as a function of dilution rate. Example 9.2 Derive the relationship between the overall mass-transfer coefficient for liquid phase K; and the individual mass-transfer coefficients, f; and kg. How can this relationship be simplitied for sparingly soluble gases? Example 9.4 Estimate the mass-transfer coefficient for the oxygen dissolution in water 25°C in a mixing vessel equipped with flat-blade disk turbine and sparger by using Calderbank and Moo-Young's correlations.Example 9.5 A cylindrical tank (1.22 m diameter) is filled with water to an operating level equal to the tank diameter. The tank is equipped with four equally spaced batiles whose width is one tenth of the tank diameter. The tank is agitated with a 0.36 m diameter flat six-blade disk tubine. The impeller rotational speed is 2.8 rps. The air enters through an open-ended tube situated below the impeller and its volumetric flow rate is 0.00416 m3/s at 1.08 atm and 25°C. Caleulate the following properties and compare the calculated values with those experimental data reported by Chandrasekharan and Calderbank (1981): Py, = 697 W: H= 0.02; kya = 0.0217 s* a. Power requirement b. Gas hold-up ¢. Sauter-mean diameter d. Interfacial area e. Volumetric mass-transfer coefficient